Cryopreservation of human immune cells
Cell cryopreservation is an essential tool for managing variability associated with human immune cell stability by locking in viability and providing researchers with reliable, on-demand human immune cells for basic research or cell therapy development.
What is cell cryopreservation?
Cryopreservation is a technique to preserve living cells, tissues, or organisms. This process involves rapidly cooling biological material to halt all metabolic activity and preserve it in a state of suspended animation. Cryoprotective agents minimize the damaging effects of ice formation and cell dehydration, allowing for long-term storage of valuable biological samples.
Key benefits of cell cryopreservation
- Preserves cell quality and viability—cell cryopreservation processes extend human immune cells' shelf life and stability, preserving cell viability and functionality until the cells are needed.
- Mitigates risk and simplifies complex logistics—cell cryopreservation protects human immune cell quality and efficacy, reduces shipping risks, and safeguards the product against unexpected shipping delays.
- Allows for proper planning & allocation of supplies—effectively plan and manage processing materials in advance. Cell cryopreservation helps reduce costs and material waste due to the shelf life of products or inadequate planning.
Safeguarding cell quality with cryopreservation
It is important to have optimized processes and expertise to ensure cell viability and functionality during cryopreservation, as some cell types are more sensitive to the cryopreservation process than others. For cryopreservation to be successful, the freezing, storage, and thawing protocol must be carried out correctly.
Improper cryopreservation can lead to a significant loss of cell viability and function, which has made scientists cautious about using cryopreserved products in the past. Charles River has spent the past decade perfecting our cryopreservation protocols to ensure maximum viability and functionality. As a result, we marketed the first commercially available cryopreserved leukopaks.
Cell cryopreservation expertise at Charles River
When done right, cryopreservation simplifies shipping and storage logistics while maintaining cell quality and stability, helps to ensure product consistency, and allows scheduling flexibility when planning and coordinating downstream processing activities. Cell cryopreservation expertise at Charles River includes:
- On-site immune cell collections with multiple collection sites
- Same-day cell processing, isolation, and cryopreservation
- Optimized procedures using rate-controlled cryopreservation techniques to ensure optimal cell viability and recovery
- Cryopreserved cells have >90% viability for the majority of cell types measured by flow cytometry, with guaranteed cell counts
- Well-integrated workflows designed to minimize room temperature exposure and freeze-thaw cycles
30% discount on human immune and stem cells from Charles River through March 31, 2024.
Available products include:
- Cryopreserved leukopaks
- PBMCs
- CD34+ stem cells
- CD56+ NK cells
- CD4+ and CD8+ T cells
- B cells
- Neutrophils
To learn more, visit the Charles River Cell Solutions storefront on Science Exchange.