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Lora Barsky

Director - USC Flow Cytometry Core
Los Angeles California, US
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Position - Company  
Manager, Flow Cytometry Core - CHILDRENS HOSPITAL

Supervise and manage a busy sorting lab.
Coordinate flow cytometric needs of Saban Research Institute
Lead Sort Operator
Deliver educational and training seminars.
Proficient with instrumentation: 3-laser DiVa FACSVantage SE, 3-laser FACSAria-I, 2-laser FACSCalibur, and FACScan.
Developing skills: Amnis Imagestream
Maintain and troubleshoot instrumentation.
Provide user training for data acquisition and interpretation.

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Dec 1995 - Oct 2009
Laboratory technician - SPECIALITY LABORATORIES, INC

Immunophenotyping for Leukemia/Lymphoma specimens,
Cell cycle analysis of solid tumors,
Enzyme immunoassay of breast tumors.

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Jan 1994 - Dec 1995

Oversee S. purpuratus embryos, young adults and breeders.
Media preparation and mortality logs.

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Aug 1991 - Jan 1994
Director - USC Flow Cytometry Core University of Southern California Oct 2009 - Present
Member - International Society for the Advancement of Cytometry Jan 1995 - Present
Degree - University  
B.S, Biological Sciences - University of California, Irvine 1989 - 1993
Publication Info
IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow. Parrish YK, Baez I, Milford TA, Benitez A, Galloway N, Rogerio JW, Sahakian E and 10 others J Immunol. 182(7):4255-66. doi: 10.4049/jimmunol.0800489. April 1, 2009. View on PubMed.
Lung alveolar integrity is compromised by telomere shortening in telomerase-null mice. Lee J, Reddy R, Barsky L, Scholes J, Chen H, Shi W, Driscoll B Am J Physiol Lung Cell Mol Physiol. 296(1):L57-70. doi: 10.1152/ajplung.90411.2008. October 24, 2008. View on PubMed.
TGF-beta signaling promotes survival and repair in rat alveolar epithelial type 2 cells during recovery after hyperoxic injury. Buckley S, Shi W, Barsky L, Warburton D Am J Physiol Lung Cell Mol Physiol. 294(4):L739-48. doi: 10.1152/ajplung.00294.2007. April 2008. View on PubMed.
Human intrathymic lineage commitment is marked by differential CD7 expression: identification of CD7- lympho-myeloid thymic progenitors. Hao QL, George AA, Zhu J, Barsky L, Zielinska E, Wang X, Price M, Ge S, Crooks GM Blood. 111(3):1318-26. doi: 10.1182/blood-2007-08-106294. February 1, 2008. View on PubMed.
Importance of interleukin-7 in the development of experimental graft-versus-host disease. Chung B, Dudl E, Toyama A, Barsky L, Weinberg KI Biol Blood Marrow Transplant. 14(1):16-27. doi: 10.1016/j.bbmt.2007.07.015. December 3, 2007. View on PubMed.
Prevention of graft-versus-host disease by anti IL-7Ralpha antibody. Chung B, Dudl EP, Min D, Barsky L, Smiley N, Weinberg KI Blood. 110(8):2803-10. doi: 10.1182/blood-2006-11-055673. October 15, 2007. View on PubMed.
A CD133-expressing murine liver oval cell population with bilineage potential. Rountree CB, Barsky L, Ge S, Zhu J, Senadheera S, Crooks GM Stem Cells. 25(10):2419-29. doi: 10.1634/stemcells.2007-0176. June 21, 2007. View on PubMed.
Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Rountree CB, Wang X, Ge S, Barsky L, Zhu J, Gonzales I, Crooks GM Hepatology. 45(5):1250-60. doi: 10.1002/hep.21600. May 2007. View on PubMed.
Stable gene transfer to human CD34(+) hematopoietic cells using the Sleeping Beauty transposon. Hollis RP, Nightingale SJ, Wang X, Pepper KA, Yu XJ, Barsky L, Crooks GM, Kohn DB Exp Hematol. 34(10):1333-43. doi: 10.1016/j.exphem.2006.05.023. October 2006. View on PubMed.
Retinoic acid induces leukemia cell G1 arrest and transition into differentiation by inhibiting cyclin-dependent kinase-activating kinase binding and phosphorylation of PML/RARalpha. Wang JG, Barsky LW, Davicioni E, Weinberg KI, Triche TJ, Zhang XK, Wu L FASEB J. 20(12):2142-4. doi: 10.1096/fj.06-5900fje. August 25, 2006. View on PubMed.
The role of the hyaluronan receptor CD44 in mesenchymal stem cell migration in the extracellular matrix. Zhu H, Mitsuhashi N, Klein A, Barsky LW, Weinberg K, Barr ML, Demetriou A, Wu GD Stem Cells. 24(4):928-35. doi: 10.1634/stemcells.2005-0186. November 23, 2005. View on PubMed.
Contribution of proliferation and DNA damage repair to alveolar epithelial type 2 cell recovery from hyperoxia. Lee J, Reddy R, Barsky L, Weinberg K, Driscoll B Am J Physiol Lung Cell Mol Physiol. 290(4):L685-L694. doi: 10.1152/ajplung.00020.2005. November 18, 2005. View on PubMed.
Contribution of mesenchymal progenitor cells to tissue repair in rat cardiac allografts undergoing chronic rejection. Wu GD, Bowdish ME, Jin YS, Zhu H, Mitsuhashi N, Barsky LW, Barr ML J Heart Lung Transplant. 24(12):2160-9. doi: 10.1016/j.healun.2005.05.017. September 15, 2005. View on PubMed.
SCL expression at critical points in human hematopoietic lineage commitment. Zhang Y, Payne KJ, Zhu Y, Price MA, Parrish YK, Zielinska E, Barsky LW, Crooks GM Stem Cells. 23(6):852-60. doi: 10.1634/stemcells.2004-0260. July 2005. View on PubMed.
In vivo inosine protects alveolar epithelial type 2 cells against hyperoxia-induced DNA damage through MAP kinase signaling. Buckley S, Barsky L, Weinberg K, Warburton D Am J Physiol Lung Cell Mol Physiol. 288(3):L569-75. doi: 10.1152/ajplung.00278.2004. December 3, 2004. View on PubMed.
Isolation of a putative progenitor subpopulation of alveolar epithelial type 2 cells. Reddy R, Buckley S, Doerken M, Barsky L, Weinberg K, Anderson KD, Warburton D, Driscoll B Am J Physiol Lung Cell Mol Physiol. 286(4):L658-67. doi: 10.1152/ajplung.00159.2003. August 15, 2003. View on PubMed.
Ikaros isoform x is selectively expressed in myeloid differentiation. Payne KJ, Huang G, Sahakian E, Zhu JY, Barteneva NS, Barsky LW, Payne MA, Crooks GM J Immunol. 170(6):3091-8. March 15, 2003. View on PubMed.
Retinoid-induced G1 arrest and differentiation activation are associated with a switch to cyclin-dependent kinase-activating kinase hypophosphorylation of retinoic acid receptor alpha. Wang J, Barsky LW, Shum CH, Jong A, Weinberg KI, Collins SJ, Triche TJ, Wu L J Biol Chem. 277(45):43369-76. doi: 10.1074/jbc.M206792200. November 8, 2002. View on PubMed.
Vascular endothelial cell apoptosis induced by anti-donor non-MHC antibodies: a possible injury pathway contributing to chronic allograft rejection. Wu GD, Jin YS, Salazar R, Dai WD, Barteneva N, Barr ML, Barsky LW, Starnes VA, Cramer DV J Heart Lung Transplant. 21(11):1174-87. November 2002. View on PubMed.
alpha v-Integrin antagonist EMD 121974 induces apoptosis in brain tumor cells growing on vitronectin and tenascin. Taga T, Suzuki A, Gonzalez-Gomez I, Gilles FH, Stins M, Shimada H, Barsky L, Weinberg KI, Laug WE Int J Cancer. 98(5):690-7. April 10, 2002. View on PubMed.
The AFT024 stromal cell line supports long-term ex vivo maintenance of engrafting multipotent human hematopoietic progenitors. Nolta JA, Thiemann FT, Arakawa-Hoyt J, Dao MA, Barsky LW, Moore KA, Lemischka IR, Crooks GM Leukemia. 16(3):352-61. doi: 10.1038/sj.leu.2402371. March 2002. View on PubMed.
Radiosensitivity of thymic interleukin-7 production and thymopoiesis after bone marrow transplantation. Chung B, Barbara-Burnham L, Barsky L, Weinberg K Blood. 98(5):1601-6. September 1, 2001. View on PubMed.
Cutting edge: predominant expression of a novel Ikaros isoform in normal human hemopoiesis. Payne KJ, Nicolas JH, Zhu JY, Barsky LW, Crooks GM J Immunol. 167(4):1867-70. August 15, 2001. View on PubMed.
Identification of a novel, human multilymphoid progenitor in cord blood. Hao QL, Zhu J, Price MA, Payne KJ, Barsky LW, Crooks GM Blood. 97(12):3683-90. June 15, 2001. View on PubMed.
MAT1-modulated CAK activity regulates cell cycle G(1) exit. Wu L, Chen P, Shum CH, Chen C, Barsky LW, Weinberg KI, Jong A, Triche TJ Mol Cell Biol. 21(1):260-70. doi: 10.1128/MCB.21.1.260-270.2001. January 2001. View on PubMed.
IL-3 increases production of B lymphoid progenitors from human CD34+CD38- cells. Crooks GM, Hao QL, Petersen D, Barsky LW, Bockstoce D J Immunol. 165(5):2382-9. September 1, 2000. View on PubMed.
Spontaneous apoptosis in lymphocytes from patients with Wiskott-Aldrich syndrome: correlation of accelerated cell death and attenuated bcl-2 expression. Rawlings SL, Crooks GM, Bockstoce D, Barsky LW, Parkman R, Weinberg KI Blood. 94(11):3872-82. December 1, 1999. View on PubMed.
ERK activation protects against DNA damage and apoptosis in hyperoxic rat AEC2. Buckley S, Driscoll B, Barsky L, Weinberg K, Anderson K, Warburton D Am J Physiol. 277(1 Pt 1):L159-66. July 1999. View on PubMed.
Discovery of a regulatory motif that controls the exposure of specific upstream cyclin-dependent kinase sites that determine both conformation and growth suppressing activity of pRb. Driscoll B, T'Ang A, Hu YH, Yan CL, Fu Y, Luo Y, Wu KJ and 6 others J Biol Chem. 274(14):9463-71. April 2, 1999. View on PubMed.
Abrogation of cyclin D1 expression predisposes lung cancer cells to serum deprivation-induced apoptosis. Driscoll B, Buckley S, Barsky L, Weinberg K, Anderson KD, Warburton D Am J Physiol. 276(4 Pt 1):L679-87. April 1999. View on PubMed.
RNA antisense abrogation of MAT1 induces G1 phase arrest and triggers apoptosis in aortic smooth muscle cells. Wu L, Chen P, Hwang JJ, Barsky LW, Weinberg KI, Jong A, Starnes VA J Biol Chem. 274(9):5564-72. February 26, 1999. View on PubMed.
A multi-purpose system for water purification and sea-water softening. Barsky L, Rubinstein J, Barsky S, Kirzhner F, Bodul O Rev Environ Health. 13(4):205-12. December 1998. View on PubMed.
T lymphocytes with a normal ADA gene accumulate after transplantation of transduced autologous umbilical cord blood CD34+ cells in ADA-deficient SCID neonates. Kohn DB, Hershfield MS, Carbonaro D, Shigeoka A, Brooks J, Smogorzewska EM, Barsky LW and 16 others Nat Med. 4(7):775-80. July 1998. View on PubMed.
An in vitro model of human erythropoiesis for the study of hemoglobinopathies. Malik P, Barsky LL, Zeng L, Hiti AL, Fisher TC Ann N Y Acad Sci. 850:382-5. June 30, 1998. View on PubMed.
In vitro identification of single CD34+CD38- cells with both lymphoid and myeloid potential. Hao QL, Smogorzewska EM, Barsky LW, Crooks GM Blood. 91(11):4145-51. June 1, 1998. View on PubMed.
Apoptosis and DNA damage in type 2 alveolar epithelial cells cultured from hyperoxic rats. Buckley S, Barsky L, Driscoll B, Weinberg K, Anderson KD, Warburton D Am J Physiol. 274(5 Pt 1):L714-20. May 1998. View on PubMed.
An in vitro model of human red blood cell production from hematopoietic progenitor cells. Malik P, Fisher TC, Barsky LL, Zeng L, Izadi P, Hiti AL, Weinberg KI, Coates TD, Meiselman HJ, Kohn DB Blood. 91(8):2664-71. April 15, 1998. View on PubMed.
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