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Dan Nguyen

Position - Company  
Manager of DNA Sequencing - Laragen, Inc.

• Improved efficiency and maintain good work flow of all incoming sample workload on a daily basis
• Implemented GLP guidelines through out our sequencing protocol in order to ensure consistent and dependable results for our customers.
• Conducted weekly team meetings to address concerns and/or issues with customer results, discuss protocol modifications and new QC guidelines.
• Regular meetings with Laragen president and Genotyping manager on the status of Sequencing quality.
• Receive, sort, and determine DNA concentration of samples for entry into Laragen LIM’s system according to SOP guidelines.
• Perform sequencing reaction for run on ABI Prism 3730
• Purify DNA using automated liquid handling robots
• Extrapolate and analyze results for quality and re-test as needed
• Perform bi-weekly maintenance on ABI instruments to ensure optimal performance and accurate data collection.
• Assist in the genotyping of digested mouse samples by performing
DNA extractions, running gel electrophoresis, and preparing necessary reagents.
• Assist with customer service issues via phone and/or email

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May 2010 - Jan 2013
Graduate Student Researcher - California State Polytechnic University, Pomona

pursuit of Master's of Science degree under Dr. Buckley investigating the effects of Echinacea purpurea ethanol extract on the murine immune cell system using C57BL/6 and CB2R KO mice as experimental model.
* Worked with mammalian cell lines, HL-60 and J77A.1, in order to study effects of Echinacea purpurea on cyotkine production.
* Responsible for maintaining CB2R KO mice colony with duties including: mating, weaning, tail clipping, genotyping (PCR, RT2-PCR) and database upkeep.

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Feb 2007 - Sep 2010
Graduate Student Researcher - California State Polytechnic University, Pomona

* Served as graduate student under Dr. Alas investigating the role of HIF-1 protein in relation to cellular mismatch repair mechanism.
* Maintained colon carcinoma cell line, HCT116 under normal and hypoxic conditions.
* Performed Western blot analysis using HCT116 cells in order to determine protein expression of HIF-1.

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Jan 2006 - Jan 2007
Chemist - Abbott Laboratories 2006 - 2007
Laboratory/Chemistry Technician - Abbott Laboratories

* Served as main support personnel for lab investigations and special projects;
worked mainly with lead Investigative Chemist.
* Performed necessary tests to update mean ranges of test values, calculated and wrote supporting data reports, organized reports of completed investigations and projects, maintained lab's accordance with GMP standards,

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Feb 2005 - Dec 2005
Laboratory Technician - University of Southern California School of Medicine

* Maintained mouse colony for post docs in laboratory.
* Separated, mated, weaned, and sacrificed mice.
* Extracted DNA via tail clippings in order to perform PCR.
* Genotyped mice using gel electrophoresis to determine viable ones for colony.
* Maintained and updated colony database on Microsoft Excel/ File maker.

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May 2004 - Oct 2004
Laboratory Technician - Sygen International Inc

*Daily processing of porcine samples. (muscle, blood, semen, hair)
*Determined genotype of piglets using PCR and gel electrophoresis.
*Performed High Quality DNA extractions using Qiagen DNeasy Tissue Kit.
*Maintained inventory of old and new porcine samples that are stored at -8*C.
*Edited official laboratory protocol manual detailing every step of the duties described above.

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Apr 2002 - Mar 2004
Laboratory Technician - The National Food Lab

*Daily processing of unprocessed and/or market ready food samples such as juice concentrate and bagged salads for micro testing
*Microbiological tests were for defacement strains of salmonella, E. coli, listeria, and staphylococcus
*Daily preparation of specific agar plates for each test mentioned above
*CFU counting of plates once removed from incubation
*General laboratory upkeep and inventory of materials

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Feb 2002 - Apr 2002
Degree - University  
M.S., Biological Sciences - California State Polytechnic University, Pomona 2006 - 2009
B.S., Biotechnology - University of California, Davis 1996 - 2001
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