Missing Avatar

Diana R. Tomchick

Professor of Biophysics, Director of the Structural Biology Core Laboratory - The University of Texas Southwestern Medical Center at Dallas View Biosketch →

Position - Company  
Research Scientist - University of Virginia

Research in structural biology/protein crystallography in the lab of Wladek Minor, Department of Molecular Physiology and Biological Physics.

Show details
Oct 1997 - Jul 2000
Postdoctoral Fellow - Purdue University

Postdoctoral research in structural biology/protein crystallography in the lab of Janet L. Smith, Department of Biology.

Show details
Mar 1993 - Sep 1997
Postdoctoral Fellow - University of Wisconsin-Madison

Postdoctoral research in structural biology/protein crystallography in the lab of Ivan Rayment, Department of Biochemistry and The Enzyme Institute. American Heart Association Postdoctoral Fellow, 1991 - 1993.

Show details
Jun 1990 - Feb 1993
Professor of Biophysics, Director of the Structural Biology Core Laboratory - The University of Texas Southwestern Medical Center at Dallas Aug 2000 - Present
Degree - University  
Doctor of Philosophy (Ph.D.), Inorganic Chemistry - University of Wisconsin-Madison

Graduate research in organometallic molybdenum/iron cluster synthesis and characterization in the lab of Lawrence F. Dahl, Department of Chemistry. Pauling Prize for best student poster presentation at the 1990 American Crystallographic Association meeting.

Show details
1983 - 1990
Bachelor of Science (B.S.), Chemistry - Washington State University

Graduated cum laude with an American Chemical Society-approved Bachelor's of Science degree in Chemistry. Undergraduate research in inorganic and physical chemistry of copper coordination compounds in the lab of Roger Willett. Received a Washington State University National Merit Scholarship for the years 1979 - 1983.

Show details
1979 - 1983
Publication Info
Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting. Kim S, Sun H, Tomchick DR, Yu H, Luo X Proc Natl Acad Sci U S A. 109(17):6549-54. doi: 10.1073/pnas.1118210109. April 24, 2012. View on PubMed.
Structural and Thermodynamic Characterization of the Interaction between Two Periplasmic Treponema pallidum Lipoproteins that are Components of a TPR-Protein-Associated TRAP Transporter (TPAT). Brautigam CA, Deka RK, Schuck P, Tomchick DR, Norgard MV J Mol Biol. :. doi: 10.1016/j.jmb.2012.04.001. April 11, 2012. View on PubMed.
Structural, bioinformatic, and in vivo analyses of two Treponema pallidum lipoproteins reveal a unique TRAP transporter. Deka RK, Brautigam CA, Goldberg M, Schuck P, Tomchick DR, Norgard MV J Mol Biol. 416(5):678-96. doi: 10.1016/j.jmb.2012.01.015. March 9, 2012. View on PubMed.
Autoinhibition of Mint1 adaptor protein regulates amyloid precursor protein binding and processing. Matos MF, Xu Y, Dulubova I, Otwinowski Z, Richardson JM, Tomchick DR, Rizo J, Ho A Proc Natl Acad Sci U S A. 109(10):3802-7. doi: 10.1073/pnas.1119075109. March 6, 2012. View on PubMed.
Structural and molecular characterization of iron-sensing hemerythrin-like domain within F-box and leucine-rich repeat protein 5 (FBXL5). Thompson JW, Salahudeen AA, Chollangi S, Ruiz JC, Brautigam CA, Makris TM, Lipscomb JD, Tomchick DR, Bruick RK J Biol Chem. 287(10):7357-65. doi: 10.1074/jbc.M111.308684. March 2, 2012. View on PubMed.
The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors. Li W, Ma C, Guan R, Xu Y, Tomchick DR, Rizo J Structure. 19(10):1443-55. doi: 10.1016/j.str.2011.07.012. October 12, 2011. View on PubMed.
A single active-site mutation of P450BM-3 dramatically enhances substrate binding and rate of product formation. Haines DC, Hegde A, Chen B, Zhao W, Bondlela M, Humphreys JM, Mullin DA, Tomchick DR, Machius M, Peterson JA Biochemistry. 50(39):8333-41. doi: 10.1021/bi201099j. October 4, 2011. View on PubMed.
Mechanism of actin filament nucleation by the bacterial effector VopL. Yu B, Cheng HC, Brautigam CA, Tomchick DR, Rosen MK Nat Struct Mol Biol. 18(9):1068-74. doi: 10.1038/nsmb.2110. August 28, 2011. View on PubMed.
The assembly of a GTPase-kinase signalling complex by a bacterial catalytic scaffold. Selyunin AS, Sutton SE, Weigele BA, Reddick LE, Orchard RC, Bresson SM, Tomchick DR, Alto NM Nature. 469(7328):107-11. doi: 10.1038/nature09593. January 6, 2011. View on PubMed.
Crystal structure of the Formin mDia1 in autoinhibited conformation. Otomo T, Tomchick DR, Otomo C, Machius M, Rosen MK PLoS One. 5(9):. doi: 10.1371/journal.pone.0012896. September 30, 2010. View on PubMed.
Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7. Xue M, Craig TK, Shin OH, Li L, Brautigam CA, Tomchick DR, Südhof TC, Rosenmund C, Rizo J PLoS One. 5(9):. doi: 10.1371/journal.pone.0012544. September 2, 2010. View on PubMed.
Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine. Deng X, Lee J, Michael AJ, Tomchick DR, Goldsmith EJ, Phillips MA J Biol Chem. 285(33):25708-19. doi: 10.1074/jbc.M110.121137. August 13, 2010. View on PubMed.
Kinetic and structural insights into the mechanism of AMPylation by VopS Fic domain. Luong P, Kinch LN, Brautigam CA, Grishin NV, Tomchick DR, Orth K J Biol Chem. 285(26):20155-63. doi: 10.1074/jbc.M110.114884. June 25, 2010. View on PubMed.
Structural and functional analysis of the YAP-binding domain of human TEAD2. Tian W, Yu J, Tomchick DR, Pan D, Luo X Proc Natl Acad Sci U S A. 107(16):7293-8. doi: 10.1073/pnas.1000293107. April 20, 2010. View on PubMed.
Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis. Shin OH, Lu J, Rhee JS, Tomchick DR, Pang ZP, Wojcik SM, Camacho-Perez M and 5 others Nat Struct Mol Biol. 17(3):280-8. doi: 10.1038/nsmb.1758. February 14, 2010. View on PubMed.
Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1. Yu B, Martins IR, Li P, Amarasinghe GK, Umetani J, Fernandez-Zapico ME, Billadeau DD, Machius M, Tomchick DR, Rosen MK Cell. 140(2):246-56. doi: 10.1016/j.cell.2009.12.033. January 22, 2010. View on PubMed.
Localization and structure of the ankyrin-binding site on beta2-spectrin. Davis L, Abdi K, Machius M, Brautigam C, Tomchick DR, Bennett V, Michaely P J Biol Chem. 284(11):6982-7. doi: 10.1074/jbc.M809245200. March 13, 2009. View on PubMed.
Artificial ligand binding within the HIF2alpha PAS-B domain of the HIF2 transcription factor. Scheuermann TH, Tomchick DR, Machius M, Guo Y, Bruick RK, Gardner KH Proc Natl Acad Sci U S A. 106(2):450-5. doi: 10.1073/pnas.0808092106. January 13, 2009. View on PubMed.
Structure and substrate recruitment of the human spindle checkpoint kinase Bub1. Kang J, Yang M, Li B, Qi W, Zhang C, Shokat KM, Tomchick DR, Machius M, Yu H Mol Cell. 32(3):394-405. doi: 10.1016/j.molcel.2008.09.017. November 7, 2008. View on PubMed.
Changes at the KinA PAS-A dimerization interface influence histidine kinase function. Lee J, Tomchick DR, Brautigam CA, Machius M, Kort R, Hellingwerf KJ, Gardner KH Biochemistry. 47(13):4051-64. doi: 10.1021/bi7021156. April 1, 2008. View on PubMed.
Crystal structure of inhibitor-bound P450BM-3 reveals open conformation of substrate access channel. Haines DC, Chen B, Tomchick DR, Bondlela M, Hegde A, Machius M, Peterson JA Biochemistry. 47(12):3662-70. doi: 10.1021/bi7023964. March 25, 2008. View on PubMed.
Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer. Yang M, Li B, Liu CJ, Tomchick DR, Machius M, Rizo J, Yu H, Luo X PLoS Biol. 6(3):e50. doi: 10.1371/journal.pbio.0060050. March 4, 2008. View on PubMed.
Enzyme structure and dynamics affect hydrogen tunneling: the impact of a remote side chain (I553) in soybean lipoxygenase-1. Meyer MP, Tomchick DR, Klinman JP Proc Natl Acad Sci U S A. 105(4):1146-51. doi: 10.1073/pnas.0710643105. January 29, 2008. View on PubMed.
Interactions of substrates at the surface of P450s can greatly enhance substrate potency. Hegde A, Haines DC, Bondlela M, Chen B, Schaffer N, Tomchick DR, Machius M and 5 others Biochemistry. 46(49):14010-7. doi: 10.1021/bi701667m. December 11, 2007. View on PubMed.
p31comet blocks Mad2 activation through structural mimicry. Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X Cell. 131(4):744-55. doi: 10.1016/j.cell.2007.08.048. November 16, 2007. View on PubMed.
Structural and biochemical basis for polyamine binding to the Tp0655 lipoprotein of Treponema pallidum: putative role for Tp0655 (TpPotD) as a polyamine receptor. Machius M, Brautigam CA, Tomchick DR, Ward P, Otwinowski Z, Blevins JS, Deka RK, Norgard MV J Mol Biol. 373(3):681-94. doi: 10.1016/j.jmb.2007.08.018. October 26, 2007. View on PubMed.
Crystal structure of the RIM1alpha C2B domain at 1.7 A resolution. Guan R, Dai H, Tomchick DR, Dulubova I, Machius M, Südhof TC, Rizo J Biochemistry. 46(31):8988-98. doi: 10.1021/bi700698a. August 7, 2007. View on PubMed.
Structural basis of histone demethylation by LSD1 revealed by suicide inactivation. Yang M, Culhane JC, Szewczuk LM, Gocke CB, Brautigam CA, Tomchick DR, Machius M, Cole PA, Yu H Nat Struct Mol Biol. 14(6):535-9. doi: 10.1038/nsmb1255. May 27, 2007. View on PubMed.
Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases. Chosed R, Tomchick DR, Brautigam CA, Mukherjee S, Negi VS, Machius M, Orth K J Biol Chem. 282(9):6773-82. doi: 10.1074/jbc.M608730200. March 2, 2007. View on PubMed.
Crystal structure of the Tp34 (TP0971) lipoprotein of treponema pallidum: implications of its metal-bound state and affinity for human lactoferrin. Deka RK, Brautigam CA, Tomson FL, Lumpkins SB, Tomchick DR, Machius M, Norgard MV J Biol Chem. 282(8):5944-58. doi: 10.1074/jbc.M610215200. February 23, 2007. View on PubMed.
Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase. Yang M, Gocke CB, Luo X, Borek D, Tomchick DR, Machius M, Otwinowski Z, Yu H Mol Cell. 23(3):377-87. doi: 10.1016/j.molcel.2006.07.012. August 4, 2006. View on PubMed.
Crystal structure of a type III pantothenate kinase: insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria. Yang K, Eyobo Y, Brand LA, Martynowski D, Tomchick D, Strauss E, Zhang H J Bacteriol. 188(15):5532-40. doi: 10.1128/JB.00469-06. August 2006. View on PubMed.
Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Lu J, Machius M, Dulubova I, Dai H, Südhof TC, Tomchick DR, Rizo J PLoS Biol. 4(7):e192. doi: 10.1371/journal.pbio.0040192. July 2006. View on PubMed.
A proximal arginine R206 participates in switching of the Bradyrhizobium japonicum FixL oxygen sensor. Gilles-Gonzalez MA, Caceres AI, Sousa EH, Tomchick DR, Brautigam C, Gonzalez C, Machius M J Mol Biol. 360(1):80-9. doi: 10.1016/j.jmb.2006.04.054. June 30, 2006. View on PubMed.
The PnrA (Tp0319; TmpC) lipoprotein represents a new family of bacterial purine nucleoside receptor encoded within an ATP-binding cassette (ABC)-like operon in Treponema pallidum. Deka RK, Brautigam CA, Yang XF, Blevins JS, Machius M, Tomchick DR, Norgard MV J Biol Chem. 281(12):8072-81. doi: 10.1074/jbc.M511405200. March 24, 2006. View on PubMed.
A versatile conformational switch regulates reactivity in human branched-chain alpha-ketoacid dehydrogenase. Machius M, Wynn RM, Chuang JL, Li J, Kluger R, Yu D, Tomchick DR, Brautigam CA, Chuang DT Structure. 14(2):287-98. doi: 10.1016/j.str.2005.10.009. February 2006. View on PubMed.
Dynamic evolution of the human immunodeficiency virus type 1 pathogenic factor, Nef. O'Neill E, Kuo LS, Krisko JF, Tomchick DR, Garcia JV, Foster JL J Virol. 80(3):1311-20. doi: 10.1128/JVI.80.3.1311-1320.2006. February 2006. View on PubMed.
Structural insight into interactions between dihydrolipoamide dehydrogenase (E3) and E3 binding protein of human pyruvate dehydrogenase complex. Brautigam CA, Wynn RM, Chuang JL, Machius M, Tomchick DR, Chuang DT Structure. 14(3):611-21. doi: 10.1016/j.str.2006.01.001. January 26, 2006. View on PubMed.
Crystal structure of the RIM2 C2A-domain at 1.4 A resolution. Dai H, Tomchick DR, García J, Südhof TC, Machius M, Rizo J Biochemistry. 44(41):13533-42. doi: 10.1021/bi0513608. October 18, 2005. View on PubMed.
Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations. Brautigam CA, Chuang JL, Tomchick DR, Machius M, Chuang DT J Mol Biol. 350(3):543-52. doi: 10.1016/j.jmb.2005.05.014. July 15, 2005. View on PubMed.
Structural basis of Rho GTPase-mediated activation of the formin mDia1. Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK Mol Cell. 18(3):273-81. doi: 10.1016/j.molcel.2005.04.002. April 29, 2005. View on PubMed.
Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain. Otomo T, Tomchick DR, Otomo C, Panchal SC, Machius M, Rosen MK Nature. 433(7025):488-94. doi: 10.1038/nature03251. February 3, 2005. View on PubMed.
Structural evidence that the 32-kilodalton lipoprotein (Tp32) of Treponema pallidum is an L-methionine-binding protein. Deka RK, Neil L, Hagman KE, Machius M, Tomchick DR, Brautigam CA, Norgard MV J Biol Chem. 279(53):55644-50. doi: 10.1074/jbc.M409263200. December 31, 2004. View on PubMed.
Molecular mechanism for regulation of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase complex by phosphorylation. Wynn RM, Kato M, Machius M, Chuang JL, Li J, Tomchick DR, Chuang DT Structure. 12(12):2185-96. doi: 10.1016/j.str.2004.09.013. December 2004. View on PubMed.
Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana. Brautigam CA, Smith BS, Ma Z, Palnitkar M, Tomchick DR, Machius M, Deisenhofer J Proc Natl Acad Sci U S A. 101(33):12142-7. doi: 10.1073/pnas.0404851101. August 17, 2004. View on PubMed.
Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4. Dai H, Shin OH, Machius M, Tomchick DR, Südhof TC, Rizo J Nat Struct Mol Biol. 11(9):844-9. doi: 10.1038/nsmb817. August 15, 2004. View on PubMed.
Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase. Li J, Wynn RM, Machius M, Chuang JL, Karthikeyan S, Tomchick DR, Chuang DT J Biol Chem. 279(31):32968-78. doi: 10.1074/jbc.M403611200. July 30, 2004. View on PubMed.
Roles of His291-alpha and His146-beta' in the reductive acylation reaction catalyzed by human branched-chain alpha-ketoacid dehydrogenase: refined phosphorylation loop structure in the active site. Wynn RM, Machius M, Chuang JL, Li J, Tomchick DR, Chuang DT J Biol Chem. 278(44):43402-10. doi: 10.1074/jbc.M306204200. October 31, 2003. View on PubMed.
The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation. Sinha SC, Krahn J, Shin BS, Tomchick DR, Zalkin H, Smith JL J Bacteriol. 185(14):4087-98. July 2003. View on PubMed.
Spectroscopic characterization of soybean lipoxygenase-1 mutants: the role of second coordination sphere residues in the regulation of enzyme activity. Schenk G, Neidig ML, Zhou J, Holman TR, Solomon EI Biochemistry. 42(24):7294-302. doi: 10.1021/bi027380g. June 24, 2003. View on PubMed.
Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6. Garrard SM, Capaldo CT, Gao L, Rosen MK, Macara IG, Tomchick DR EMBO J. 22(5):1125-33. doi: 10.1093/emboj/cdg110. March 3, 2003. View on PubMed.
Crystal structure of a 12 ANK repeat stack from human ankyrinR. Michaely P, Tomchick DR, Machius M, Anderson RG EMBO J. 21(23):6387-96. December 2, 2002. View on PubMed.
Crystal structure of the 47-kDa lipoprotein of Treponema pallidum reveals a novel penicillin-binding protein. Deka RK, Machius M, Norgard MV, Tomchick DR J Biol Chem. 277(44):41857-64. doi: 10.1074/jbc.M207402200. November 1, 2002. View on PubMed.
Structure of human nicotinamide/nicotinic acid mononucleotide adenylyltransferase. Basis for the dual substrate specificity and activation of the oncolytic agent tiazofurin. Zhou T, Kurnasov O, Tomchick DR, Binns DD, Grishin NV, Marquez VE, Osterman AL, Zhang H J Biol Chem. 277(15):13148-54. doi: 10.1074/jbc.M111469200. April 12, 2002. View on PubMed.
Three-dimensional structure of the complexin/SNARE complex. Chen X, Tomchick DR, Kovrigin E, Araç D, Machius M, Südhof TC, Rizo J Neuron. 33(3):397-409. January 31, 2002. View on PubMed.
Pivotal role of water in the mechanism of P450BM-3. Haines DC, Tomchick DR, Machius M, Peterson JA Biochemistry. 40(45):13456-65. November 13, 2001. View on PubMed.
Structure of rat BCKD kinase: nucleotide-induced domain communication in a mitochondrial protein kinase. Machius M, Chuang JL, Wynn RM, Tomchick DR, Chuang DT Proc Natl Acad Sci U S A. 98(20):11218-23. doi: 10.1073/pnas.201220098. September 25, 2001. View on PubMed.
Structural and functional characterization of second-coordination sphere mutants of soybean lipoxygenase-1. Tomchick DR, Phan P, Cymborowski M, Minor W, Holman TR Biochemistry. 40(25):7509-17. June 26, 2001. View on PubMed.
Strategies for macromolecular synchrotron crystallography. Minor W, Tomchick D, Otwinowski Z Structure. 8(5):R105-10. May 15, 2000. View on PubMed.
Adaptation of an enzyme to regulatory function: structure of Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase. Tomchick DR, Turner RJ, Switzer RL, Smith JL Structure. 6(3):337-50. March 15, 1998. View on PubMed.
Mechanism of the synergistic end-product regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase by nucleotides. Chen S, Tomchick DR, Wolle D, Hu P, Smith JL, Switzer RL, Zalkin H Biochemistry. 36(35):10718-26. doi: 10.1021/bi9711893. September 2, 1997. View on PubMed.
Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site. Kim JH, Krahn JM, Tomchick DR, Smith JL, Zalkin H J Biol Chem. 271(26):15549-57. June 28, 1996. View on PubMed.
Crystallization and preliminary X-ray crystallographic analysis of carbamoyl phosphate synthetase from Escherichia coli. Thoden JB, Raushel FM, Mareya S, Tomchick D, Rayment I Acta Crystallogr D Biol Crystallogr. 51(Pt 5):827-9. doi: 10.1107/S0907444994012801. September 1, 1995. View on PubMed.
Three-dimensional structure of myosin subfragment-1: a molecular motor. Rayment I, Rypniewski WR, Schmidt-Bäse K, Smith R, Tomchick DR, Benning MM, Winkelmann DA, Wesenberg G, Holden HM Science. 261(5117):50-8. July 2, 1993. View on PubMed.
  • Diana R. Tomchick hasn't received any ratings yet...