Service Details:
• Available for human, plants and animals
• Illumina HiSeq X Ten (PE150)
• 350 bp insert DNA library
• Q30≥80%
• Recommended coverage: 30X-50X for rare diseases; 50X for tumor tissues and 30X for adjacent normal tissues and blood

Comprehensive analysis available at a low package price.

Bioinformatics Analysis Content includes:
• Data quality control: filtering reads containing adapters or with low quality
• Alignment with reference genome
* Statistics of sequencing depth and coverage
• SNP/InDel/SV/CNV calling, annotation and statistics
• Somatic SNP/InDel/SV/CNV calling, annotation and statistics (for paired tumor samples)

Novogene is one of the first few companies in the world employing the powerful Illumina HiSeq X Ten for whole genome sequencing. We possess one of the largest NGS capacities in the world with two sets of HiSeq X Ten systems at our California and China labs. Our extensive experience and bioinformatics capabilities enable us to expertly meet customer needs with timely turn-around at highly competitive prices.

30X coverage: $2500 with data analysis
Turnaround time: 8 weeks

We provide Whole Genome Sequencing (WGS) with 30x coverage in an application ready data that can be used with popular third party genomic analysis services and genomic applications currently available on the web.

We provide genomic data, we do not provide medical advice. The intended use of our Whole Genome Sequencing services is for informational and educational use only.

Whole genome de novo sequencing

Whole genome de novo sequencing requires special library preparation and highly parallel sequencing technology. There are two types of de novo genome sequencing which are draft map and fine map. To construct successful de novo genome, various libraries with different sizes (200bp, 500bp, 2kb, 5kb, 10kb, 20kb) are usually prepared.

Whole genome resequencing

Whole genome re-sequencing is to compare genome sample to available genome reference (Initial sequence of particular genome) for detecting variants (SNPs, Indels). This requires highly parallel system such as Illumina HiSeq to provide sufficient coverage depths for accurate variant detection.

Human whole genome sequencing allows for detection of variations to discover potential correlations to certain disease risks, and it can also play a role as molecular biomarkers for disease diagnosis and prediction.

• De novo sequencing is typically performed without prior knowledge of the sequencing data, De novo sequencing has proven successful for confirming and expanding upon results from database searches, providing excellent resources for understanding a species.
  
• Some of the most crucial information, obtained by resequencing of organism’s genome DNA, are the individual variations in the genome, such as single nucleotide polymorphism(SNP), copy number variation(CNV), and structural variation
  

Sequencing platform
- HiSeq X10
- GS-FLX Titanium / Plus (shotgun, Mate pair)
- HiSeq2000 / MiSeq system (Paired-end, Mate pair)

Affiliated Genetics is a CLIA, CAP, AABB accredited laboratory located in Salt Lake City, Utah since 1994.

Whole Genome Sequencing for research only. Standard output raw data to include VCF / BAM / BAI files only.

Mean depth coverage 90x
1-4 samples - $4,150 each
5-12 samples - $4,000 each
>12 samples - $3,750 each

Mean depth coverage 30x
1-4 samples - $3,000 each
5-12 samples - $2,850 each
>12 samples - $2,500 each

*All WGS samples will be run on the Illumina HiSeq 4000.

**Raw data plus custom initial Cartagenia filtering also available for an additional fee.

***WGS can be performed using a CLIA protocol when results are to be reported to patients with a full clinical interpretation. Please call for details regarding pricing.