Whole Exome Sequencing

Whole exome sequencing (WES) is the sequencing of only the exons of a genome. The general procedure is to lyse cells and purify genomic DNA (gDNA). gDNA is then sheared into fragments and ligated to adapters specific to the sequencing platform. The fragments are amplified by... Show more »

Whole exome sequencing (WES) is the sequencing of only the exons of a genome. The general procedure is to lyse cells and purify genomic DNA (gDNA). gDNA is then sheared into fragments and ligated to adapters specific to the sequencing platform. The fragments are amplified by polymerase chain reaction (PCR) and bound to short bits of exon-specific DNA (oligos). The oligos are tagged and then affinity purified. Commonly, the oligos are biotinylated so they can be collected on streptavidin beads. The isolated exons undergo a second round of amplification by PCR and the fragments are sequenced. Alternatively, RNA can be isolated instead of DNA. This limits non-coding background since RNA is only the coding regions flanked by easily eliminated untranslated regions. WES is cheaper and faster than whole genome sequencing and captures the majority of the information related to genetic disease. Considerations for WES include sequencing platform and the method for shearing DNA to obtain the optimal fragment length for the chosen platform. Applications of WES include the study of genetic-based diseases, personalized medicine, genetic counseling, and population studies. (Credit: Brooke Anderson-White) « Show less

We found 33 labs offering this service with prices from $390.00 to $1,050.00 per sample. 100% positive ratings across 101 reviews.

Price on request
1382635948933 367e7a7d2a980c37c1ed945c8bdfc248

Genomics Shared Resource

Buffalo New York
4 Orders Completed
100% Positive
Price on request
1352601184186 7fffb663120dd173020ed4b9bc223583

Penn Genomic Analysis Core

Philadelphia Pennsylvania
4 Orders Completed
100% Positive

Whole exome sequencing on Ion Torrent Proton sequencer. Up to 80 million reads and 12Gb throughput. Results in 1 - 3 weeks.

Price on request
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Macrogen

Rockville Maryland
3 Orders Completed
100% Positive

We require 1ug of gDNA for Agilent SureSelect Human All Exon V4 (51M) or V5 (50M) capture kit, sequencing will be performed on the latest platform, Illumina HiSeq 4000 with 100X RAW DATA.

CLIA Level Sequencing

In the U.S., the Centers for Medicare & Medicaid Services (CMS) regulates all laboratory testing (except... Show more »

We require 1ug of gDNA for Agilent SureSelect Human All Exon V4 (51M) or V5 (50M) capture kit, sequencing will be performed on the latest platform, Illumina HiSeq 4000 with 100X RAW DATA.

CLIA Level Sequencing

In the U.S., the Centers for Medicare & Medicaid Services (CMS) regulates all laboratory testing (except research) performed on humans through the Clinical Laboratory Improvement Amendments (CLIA). The objective of the CLIA program is to ensure quality laboratory testing. Macrogen Clinical Laboratory has received CLIA certification from U.S. CMS and provides CLIA quality of Whole Exome Sequencing.
Macrogen Clinical Laboratory performs routine quality management procedures including laboratory condition and validation of equipment and instrument to guarantee high quality of data. Macrogen Clinical Laboratory also performs QC (quality control) with higher standard and all laboratory procedures are processed by certified personnel only.

Safety

  • Restricted laboratory access
  • CLIA level technical processes
  • Secure Protected Data
  • Secured and on-site server analysis

Enhanced Analysis

  • Customized analysis for various hereditary diseases and cancers
  • Project consultation about clinical genomic research
  • Clinical service advice
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Starting at $653.00 USD per sample
1310477538000

St. Louis Missouri
1 Order Completed
100% Positive

Agilent Exomes. Please note price varies depending on the number of samples, the library prep, and sequencing coverage.

Starting at $950.00 USD per sample
1402442844114 6a0ac92e4243f0ee638cd7733d1c8fa9

Shanghai China
69 Orders Completed

100X Whole exome sequencing on HiSeq Platform: PE 2X125, including DNA sample QC, library construction, exome capture and sequencing

  • Sample request: >1ug DNA per sample
  • Data yield: Targeting 200X coverage with >10x coverage for greater than 98% of the targeted region; output >14G PF data
  • Exome capture kit:... Show more »

100X Whole exome sequencing on HiSeq Platform: PE 2X125, including DNA sample QC, library construction, exome capture and sequencing

  • Sample request: >1ug DNA per sample
  • Data yield: Targeting 200X coverage with >10x coverage for greater than 98% of the targeted region; output >14G PF data
  • Exome capture kit: Agilent SureSelectXT Human All Exon V6
  • Data QC criteria: average Q30 > 80%
  • Deliverables: raw data (FASTQ reads), Sample QC Report, Data QC Report
  • Turnaround time: 3-4 weeks after samples pass QC

150X Coverage is $1100
200X Coverage is $1500

We are flexible and can accommodate any request. For those non-standard project, please inquire for more details.

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Price on request
1321235387000

Otogenetics Corporation

Norcross Georgia
5 Orders Completed

Whole exome sequencing using Agilent Capture and Illumina HiSeq

Human All Exon (50Mb), Human UTR, Mouse, Canine and Zebrafish Captures

Please click the link for the detail: https://www.otogenetics.com/whole-exome-sequencing

Starting at $1,000.00 USD per sample
1352513522536 1f5556688864cf05825295f331b18c03

Huntsville Alabama
4 Orders Completed

Whole Exome Sequencing for 100PE
-100x & 9Gb - $1,257/exome
-65x & 6Gb - $904/exome
-45x & 4.5Gb - $833/exome

Starting at $390.00 USD per sample
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Suwon Gyueonggi-do
3 Orders Completed

Up to 10 samples.
Only for the 1st order.
Sample requirement: 1ug of gDNA
Capture Kit: Agilent SureSelect V4 (51M) , V5 (50M)
Sequencing will be performed on the latest platform.
Illumina HiSeq 2500 with 100X RAW DATA.
TAT: 4 Weeks after QC pass
Data delivery via Theragen's FTP Link
Shipping is Free using our FedEx... Show more »

Up to 10 samples.
Only for the 1st order.
Sample requirement: 1ug of gDNA
Capture Kit: Agilent SureSelect V4 (51M) , V5 (50M)
Sequencing will be performed on the latest platform.
Illumina HiSeq 2500 with 100X RAW DATA.
TAT: 4 Weeks after QC pass
Data delivery via Theragen's FTP Link
Shipping is Free using our FedEx account number.
Exome sequencing is targeting exon regions of the genome. Entire exome is captured using specific kits prior to the sequencing process. Even though sequencing process is essentially the same, it is possible to increase sequencing depth while spending same cost because exome is only a fraction of whole genome.
*This promotion is only for Science Exchange

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Starting at $1,050.00 USD per sample
X3jv9dfysqiy2udnhapi ag color logo

Salt Lake City Utah
1 Order Completed

Affiliated Genetics is a CLIA, CAP, AABB accredited laboratory located in Salt Lake City, Utah since 1994.

Whole Exome Sequencing for research only. Standard output raw data to include VCF / BAM / BAI files only.

Mean depth coverage 100x
1-10 samples - $1,050
11-50 samples - $925
>50 samples - $800

Mean depth... Show more »

Affiliated Genetics is a CLIA, CAP, AABB accredited laboratory located in Salt Lake City, Utah since 1994.

Whole Exome Sequencing for research only. Standard output raw data to include VCF / BAM / BAI files only.

Mean depth coverage 100x
1-10 samples - $1,050
11-50 samples - $925
>50 samples - $800

Mean depth coverage 30x
1-10 samples - $725
11-50 samples - $650
>50 samples - $550

*Projects smaller than 50 samples will be run on the Ion Proton sequencing platform using Ion Hi-Q Chemistry and the P1V3 chip (see Life Technologies website for details). Projects larger than 50 samples may be run on the Illumina HiSeq 4000.

**Raw data plus custom initial Cartagenia filtering also available for an additional fee.

**Clinical Exome Sequencing with full interpretation is also available through Affiliated Genetics. Please call for details regarding pricing.

****TURN AROUND TIME FOR LESS THAN 50 SAMPLES 3 WEEKS

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Starting at $110.00 USD per hour
1324437006000

Hercules California
No Reviews Available