Nano-LC-MS

Nano liquid chromatography-mass spectrometry

We found 3 labs offering this service

Price on request
1406603809282 bdd712c348acb651219794d947b7dc60

System Biosciences

Mountain View California
1 Order Completed

SBI routinely generates high quality exosome peptide libraries for Mass spec analysis. The Mass Spec service works with exosomes isolated using ultracentrifugation as well as using ExoQuick (serum, plasma, ascites samples) or ExoQuick-TC (cell media, urine, spinal fluid) or immunopurified for specific exosome subpopulations using... Show more »

SBI routinely generates high quality exosome peptide libraries for Mass spec analysis. The Mass Spec service works with exosomes isolated using ultracentrifugation as well as using ExoQuick (serum, plasma, ascites samples) or ExoQuick-TC (cell media, urine, spinal fluid) or immunopurified for specific exosome subpopulations using SBI's Exo-Flow IP kits. The MS service offers the choice of creating either exosome surface protein "shaving" peptide libraries for surface marker discovery or complete exosome peptide library preparations. The peptide libraries are then analyzed by nano LC/MS/MS with a Waters NanoAcquity HPLC system interfaced to a ThermoFisher Q Exactive. Peptides were loaded on a trapping column and eluted over a 75um analytical column at 350nL/min using a 2hr reverse phase gradient; both columns were packed with Jupiter Proteo resin (Phenomenex). The injection volume was 30uL. The mass spectrometer was operated in datadependent mode, with the Orbitrap operating at 60,000 FWHM and 17,500 FWHM for MS and MS/MS respectively. The fifteen most abundant ions were selected for MS/MS and data analyzed using MASCOT databases and Scaffold software.

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1335914993000

Australian Proteome Analysis Facility

Macquarie Park New South Wales
No Reviews Available

Phosphopeptide analysis by nano-LC/MS/MS:
The analysis of phosphopeptides from complex protein mixtures remains a challenging procedure. APAF uses an Applied Biosystems 4000 Q-Trap hybrid quadrupole linear ion-trap instrument. This instrument is well suited to the analysis of post-translational modifications as diagnostic... Show more »

Phosphopeptide analysis by nano-LC/MS/MS:
The analysis of phosphopeptides from complex protein mixtures remains a challenging procedure. APAF uses an Applied Biosystems 4000 Q-Trap hybrid quadrupole linear ion-trap instrument. This instrument is well suited to the analysis of post-translational modifications as diagnostic fragment ions can be detected by precursor ion scanning. In this scan type, Q1 is scanned across a mass range and ions are fragmented in Q2. Q3 is set to transmit only the mass of the diagnostic fragment, (-79 m/z in the case of a phosphopeptide). In a follow-up experiment, precursor ions are selected from fragmentation and product ions scanned out of the ion-trap to provide MS/MS sequence information. Often the site of phosphorylation can be determined from this spectra.

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Protea Biosciences, Inc.

Morgantown West Virginia
No Reviews Available