Nano liquid chromatography-mass spectrometry (Nano-LC-MS)

We found 2 labs offering this service

Price on request
1339565308000

Biomolecular Frontiers Research Centre

North Ryde, New South Wales
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Applied Biosystems 5500 QTRAP - nanoLC and CapLC ESI MS/MS System

Price on request
1335914993000

Australian Proteome Analysis Facility
1335914993000
Australian Proteome Analysis FacilityMacquarie UniversityMacquarie Park, New South Wales
  • 32 Services
  • 2 Members

Macquarie Park, New South Wales
1335914993000
Australian Proteome Analysis FacilityMacquarie UniversityMacquarie Park, New South Wales
  • 32 Services
  • 2 Members
  • No reviews
  • No completed projects
  • No endorsements

Phosphopeptide analysis by nano-LC/MS/MS:
The analysis of phosphopeptides from complex protein mixtures remains a challenging procedure. APAF uses an Applied Biosystems 4000 Q-Trap hybrid quadrupole linear ion-trap instrument. This instrument is well suited to the analysis of post-translational modifications as diagnostic... Show more »

Phosphopeptide analysis by nano-LC/MS/MS:
The analysis of phosphopeptides from complex protein mixtures remains a challenging procedure. APAF uses an Applied Biosystems 4000 Q-Trap hybrid quadrupole linear ion-trap instrument. This instrument is well suited to the analysis of post-translational modifications as diagnostic fragment ions can be detected by precursor ion scanning. In this scan type, Q1 is scanned across a mass range and ions are fragmented in Q2. Q3 is set to transmit only the mass of the diagnostic fragment, (-79 m/z in the case of a phosphopeptide). In a follow-up experiment, precursor ions are selected from fragmentation and product ions scanned out of the ion-trap to provide MS/MS sequence information. Often the site of phosphorylation can be determined from this spectra.

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