Molecular cloning

Save 91% compared with the cost of cloning molecular constructs in your lab.

We found 29 labs offering this service with prices from $150.00 to $726.81 per sample.

$300.00 USD
per Sample
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Marietta, Georgia
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Integrated Technology Enterprise, IncMarietta, Georgia
  • 1 Service
  • 1 Member
  • 100% Positive Reviews
  • 22 Projects Completed
  • No endorsements

We clone everything:
Mutations, deletions
Epitope tags: N-, C-terminal, and between the protein domains
Protein-protein fusions
si-RNA constructs
Mice knock-out, knock-in, and transgenic constructs

$150.00 USD
per Sample
1313462337000

Raleigh, North Carolina
1313462337000
Celplor LLCRaleigh, North Carolina
  • 15 Services
  • 1 Member
  • 100% Positive Reviews
  • 8 Projects Completed
  • No endorsements

Regular cDNA cloning starts from $150.00 depending on the size of the insert. Price includes primer design and synthesis, sequencing verification of 5' and 3' ends of insert.

Deliverables:
- one mini-prep
- one glycerol stock
- electronic report with sequencing chromatograms
- samples... Show more »

Regular cDNA cloning starts from $150.00 depending on the size of the insert. Price includes primer design and synthesis, sequencing verification of 5' and 3' ends of insert.

Deliverables:
- one mini-prep
- one glycerol stock
- electronic report with sequencing chromatograms
- samples returned upon request

Turnaround time: 10-15 business days

Please include the following in your quote request:
- sample size
- vector
- template
- concentration

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$300.00 USD
per Clone
1314212146000

Woodbridge, Connecticut
1314212146000
Shakti BioResearch LLCWoodbridge, Connecticut
  • 18 Services
  • 1 Member
  • 100% Positive Reviews
  • 28 Projects Completed
  • No endorsements

Subcloning $300

PCR based cloning $0.25/bp (Minimum 1kb gene)

Price on request
1337644128000

Mouse Biology Program (MBP)

Davis, California
  • 100% positive reviews
  • 4 Projects Completed
  • No endorsements

Using BAC recombineering, we can generate constructs in as little as 6 weeks from initial consultation to final sequencing verification. By performing restriction analyses verification at key steps of the process, we have increased the quality and reliability, as well as the speed, of vector construction. We can create BAC transgenic... Show more »

Using BAC recombineering, we can generate constructs in as little as 6 weeks from initial consultation to final sequencing verification. By performing restriction analyses verification at key steps of the process, we have increased the quality and reliability, as well as the speed, of vector construction. We can create BAC transgenic constructs for uncharacterized promoters. This technique allows insertion of large (>20 kb) genomic sequences from BACs into smaller vectors suitable for gene targeting. This process bypasses the restraints of conventional ligations to produce longer genomic “arms” to increase the efficiency of homologous recombination in ES cells. Moreover, PCR error in these arms is circumvented since genomic DNA in the BAC clones is manipulated to generate the DNA constructs. Site-directed mutagenesis, point mutations, or any kind of Knock-In constructs pose no problems for this technology.

We have the capacity and flexibility to tailor-make elements for targeting and transgenic constructs (including BAC transgenic). Additionally, we are currently working at implementing a yeast system to perform many steps even more rapidly and reliably.

To get started, we need a gene name and mutant model type – i.e. KO, KI, transgenic, etc. We will put together a design and send for your approval. Before ordering primers, we require payment in the form of Purchase Order, to be provided by the science exchange.
We provide everything else.

Here is a summary of what we provide per construct:
-Thorough informatics
-Maps/sequences of final construct, all intermediate vectors, mutant locus, etc.
-Design for model
-Final plasmid sent on filter paper
-Raw sequencing data of final construct
-Upon request, we can also provide (at no additional cost):
-Figure-usable schematic
-Methods section-we suggest acknowledgment or authorship on publication
-Glycerol stock of final plasmid (if courier account number is provided)
-Glycerol stock of parent BAC clone (if courier account number is provided)
-Gel images of:
PCR verification steps
Restriction Verification steps

-Construct verification:
For final verification of targeting constructs, we perform 5 verification digests and sequencing over all insert junctions (6 junctions total for conditional KO).

For targeting constructs, the final construct is in a high-copy plasmid, so there is no need to culture BAC clones and extract DNA from BAC clones.

For BAC constructs, we provide sequencing verification across all insert junctions.

Library of available cassettes:

• LacZ
• Luciferase
• EGFP
• YFP
• dsRED
• ECFP
• Cre-ERT2 (Tamoxifen-Inducible Cre)
• Cre
• FLPe
• Tet-On (aka rtTA [reverse transcriptase transactivator])
• TRE2 (aka Tet-Responsive Element2, or “tet-O-”)
• PGK-NEO
• DTA
• mCherry
• Tomato
• iCre
• Puromcyin
• BetaActin-NEO
• Dre-recombinase

• Antibody-based tags such as:
o FLAG tag
o HA tag

• Bicistronic expression connectors such as:
o 2A peptide sequence
o IRES

Conditional Gene-trap cassette
Floxed STOP cassettes

We can custom design these to include an upstream IRES, loxP sites, frt sites, rox sites, bacterial selection cassettes, mammalian selection cassettes, etc.

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Price on request
1371590680511_a2c33e6508cb526c0b1970ac29d00e98

Alamo Laboratories Inc
1371590680511_a2c33e6508cb526c0b1970ac29d00e98
Alamo Laboratories IncSan Antonio, Texas
  • 24 Services
  • 1 Member

San Antonio, Texas
1371590680511_a2c33e6508cb526c0b1970ac29d00e98
Alamo Laboratories IncSan Antonio, Texas
  • 24 Services
  • 1 Member
  • 100% positive reviews
  • 23 Projects Completed
  • No endorsements

Cloning & Sub-cloning Service

Constructs for Adenovirus Expression System
It is a very strong and efficient system for the simultaneous expression of two or more specific gene(s) in mammalian cells. We provide this service for the construction of your gene of interest. Toxin-free final product is ready to transfect... Show more »

Cloning & Sub-cloning Service

Constructs for Adenovirus Expression System
It is a very strong and efficient system for the simultaneous expression of two or more specific gene(s) in mammalian cells. We provide this service for the construction of your gene of interest. Toxin-free final product is ready to transfect HEK cells for the production of recombinant adenovirus.

Constructs for Baculovirus Expression System
One of the most widely used gene(s) expression system for production of recombinant proteins in Eukaryotic cells (such as sf9, Sf21). We offer cloning services for the construction of gene of your interest in baculovirus-based expression vector(s). Final product is ready to transfect insect cells for the packaging of recombinant baculovirus.

Construction of Gene-specific SiRNA
This revolutionary technique to block the expression of a specific protein(s) in cells or animals has proven to be a reliable and quick approach. We'll take care of your SiRNA construct from designing of the specific primers to its cloning in an SiRNA expression vector. We also provide SiRNA duplex synthesis service with desired modifications such as biotin.

Sub-cloning
This service is provided for sub-cloning of gene/cDNA into vector of your choice. For accuracy, recombinant clones will be verified by restriction analysis. A project report with detail analysis and 10 ug of final product will be supplied. Customer supplies the specific gene/ cDNA and vector.

PCR Cloning
We'll design and make gene-specific primers for PCR amplification from a cDNA library. We'll buy commercially available specific cDNA library if customer can not provide it. PCR amplified cDNA fragment will be sub-cloned in TA cloning vector or in a vector of your choice. Recombinant clones will be verified by restriction fragment analysis. For accuracy, two selected clones will be sequenced. A project report with detail analysis and 10 ug of final product will be supplied.

RT-PCR
We'll reverse transcribe and PCR amplify specific gene from customer’s provided total RNA/mRNAs. Amplified fragment will be ligated in TA vector or in vector of your choice. Two clones will be sequenced for the validity of clones. A project report with detail analysis and 10 ug of final product will be supplied.

cDNA Library Construction
We offer cDNA library construction service at very competitive price.

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$162.00 USD
per Run
1352856131117_a2336ed1c2cc707a7d464a3906810725

Chapel Hill, North Carolina
  • 100% Positive Reviews
  • 8 Projects Completed
  • No endorsements

Clone library creation:
In order to confirm bacterial identity, PCR-amplified 16S rDNA fragments can be cloned into plasmids and transformed into an appropriate host (usually E. coli). Each transformed E. coli colony will contain only one 16S rDNA PCR-originated fragment from the original sample. A number of colonies are picked... Show more »

Clone library creation:
In order to confirm bacterial identity, PCR-amplified 16S rDNA fragments can be cloned into plasmids and transformed into an appropriate host (usually E. coli). Each transformed E. coli colony will contain only one 16S rDNA PCR-originated fragment from the original sample. A number of colonies are picked randomly and the insert is sequenced. The DNA sequence is then compared to a database (for example RDP II) for identification by DNA homology.

Equipment:
Genetix QPix2 XT Colony Picker System

UNC/NC State/ Covered by federal funds $480
Non-UNC or NC State or not covered by federal funds $528

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$295.00 USD
per Sample
1352615934490_7fffb663120dd173020ed4b9bc223583

University Heights, Ohio
1352615934490_7fffb663120dd173020ed4b9bc223583
Custom DNA ConstructsUniversity Heights, Ohio
  • 1 Service
  • 1 Member
  • 100% Positive Reviews
  • 6 Projects Completed
  • No endorsements

We charge $295/plasmid this includes cloning of a single insert and addition of ~20 bases of new sequence (no template required). Addition of longer sequences cost $10 per 20 bases. Cloning of Inserts longer than 4kb or cloning of more than one insert may result in additional charges, please request a quote. Sequencing charges are... Show more »

We charge $295/plasmid this includes cloning of a single insert and addition of ~20 bases of new sequence (no template required). Addition of longer sequences cost $10 per 20 bases. Cloning of Inserts longer than 4kb or cloning of more than one insert may result in additional charges, please request a quote. Sequencing charges are $15 per 800 bp (covers primers and sequencing costs)*.

Our service: Sub-clone DNA into plasmids according to customer's request.

Sub-clone your gene to another plasmid, make any sequence modification to your gene or plasmid. Our procedures allow cloning not only by using restriction enzymes but also in restriction-enzymes-independent ways. This allows sequence modification in areas where no suitable restriction sites are present.
Examples for services offered:

Amplification of insert from one plasmid and cloning in another plasmid.
Addition of sequence to an existing plasmid, for example adding FLAG+myc tags, restriction sites, linkers, shRNA coding sequences.
Introducing site directed mutations.
Deletion of sequences of any length from a plasmid.
Insertion of one gene fragment in to another gene, for example for making a chimeric protein.
Cloning an insert in to a plasmid while adding a tag at the 5' or 3' ends of the insert.
Cloning an insert while adding a polyglycine spacer to prevent structural effects of tags on your protein.
Any other sequence modifications you request.

* Please request a specific quote in the following cases:
Cloning into viral vectors.
Cloning of repeated sequences.
Cloning of AT- or GC-rich sequences.
Cloning of Inserts longer than 3.5 kb.
Site directed mutagenesis in plasmids larger than 9 kb.
Cloning to vectors with no full sequence information.
Cloning of more than one insert.

How it works:
Send us description of your source DNA and how you want it cloned.
Mail us your source plasmid/s.

How to ship samples:
Please provide 5 micrograms of the source plasmid/s as purified DNA good for sequencing at concentration ≥0.2 mg/ml. Miniprep DNA is fine. Please use overnight carrier with tracking service i.e. FedEx, UPS, DHL and ship at ambient temperature. Forward us the tracking number if possible.

We send you the completed plasmid and sequencing information in 2 - 4 weeks.

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Price on request
1352591431482_a2336ed1c2cc707a7d464a3906810725

Proteomics Core Facility
1352591431482_a2336ed1c2cc707a7d464a3906810725
Proteomics Core FacilityCenter for Functional GenomicsRensselaer, New York
  • 41 Services
  • 1 Member

Rensselaer, New York
1352591431482_a2336ed1c2cc707a7d464a3906810725
Proteomics Core FacilityCenter for Functional GenomicsRensselaer, New York
  • 41 Services
  • 1 Member
  • 100% positive reviews
  • 4 Projects Completed
  • No endorsements

construction of transgenic or knock out insertion vector

$195.00 USD
per Sample
1379046072341_d4243b2d6ca86e479e25a1746e1ac940

Mountain View, California
1379046072341_d4243b2d6ca86e479e25a1746e1ac940
ProNovus Bioscience, LLCMountain View, California
  • 23 Services
  • 2 Members
  • No reviews
  • 2 Projects Completed
  • No endorsements

We provide services which include but not limited to the following (please inquire about cost and service details):

-site directed mutagenesis
-cDNA cloning of species orthologs
-vector construction (N-, C-, internal tags) for affinity purification and FRET studies
-creating gene chimeras
-subcloning... Show more »

We provide services which include but not limited to the following (please inquire about cost and service details):

-site directed mutagenesis
-cDNA cloning of species orthologs
-vector construction (N-, C-, internal tags) for affinity purification and FRET studies
-creating gene chimeras
-subcloning from one vector to another (restriction enzyme cloning or Gateway)

Subcloning as low as $195.

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Price on request
1369150837652_84e7af538b4885746f7f4ddfccc03245

TransViragen
1369150837652_84e7af538b4885746f7f4ddfccc03245
TransViragenDurham, North Carolina
  • 27 Services
  • 1 Member

Durham, North Carolina
1369150837652_84e7af538b4885746f7f4ddfccc03245
TransViragenDurham, North Carolina
  • 27 Services
  • 1 Member
  • No reviews
  • No completed projects
  • No endorsements

TransViragen offers a selection of DNA cloning and recombineering services. Pricing varies depending on the nature of the needed modifications.

97% positive ratings across 30 reviews.