Mass Spectrometry

Mass spectrometry is used to determine the mass, and thus identity, of a sample molecule. Ionized molecules passing it through a strong magnetic field will have their trajectories deflected by an amount proportional to their mass to charge ratio (m/z). This m/z spectrum can then be... Show more »

Mass spectrometry is used to determine the mass, and thus identity, of a sample molecule. Ionized molecules passing it through a strong magnetic field will have their trajectories deflected by an amount proportional to their mass to charge ratio (m/z). This m/z spectrum can then be used to identify the molecule by comparison to a vast database of known molecular m/z spectra.
To accomplish this, a mass spectrometry experiment is broken down into three basic steps. First, the sample is ionized. (If the sample is already a gas, it can be directly ionized; if it is a liquid or solid it must first be vaporized before ionization.) The ions are next organized into a stream that is guided through the mass analyzer, where they are exposed to magnetic fields that deflect their trajectory based on their mass. This deflection is recorded by the mass detector.
There are many varied ways to ionize your sample. The process of ionizing a molecule can also break that molecule into fragments, in many cases in predictable patterns based on bond strengths and various functional groups that can yield structural information about the sample molecule. Hard ionization methods tend to produce lots of fragmentation, while soft ionization methods result in much less fragmentation. Thus, the m/z spectra will reflect the masses of the ionized sample analyte as well as ionized fragments of that analyte, resulting in a unique “fingerprint” for that molecule.
There are also multiple methods for perturbing the ions’ flight and detecting the ions. Combining different methods of ionization, deflection and detection make mass spectrometry a versatile technique with relevance across a broad range of scientific areas. (Credit: Fraser Tan) « Show less

We found 43 labs offering this service 100% positive ratings across 3 reviews.

$40.00 USD per Sample
1379874917605 1bb87d41d15fe27b500a4bfcde01bb0e

Catonsville, Maryland
100% Positive Reviews
24 Orders Completed

Our main tool for LC-MS services is a Waters/Micromass Quattro triple quadrupole tandem mass spectrometer. The separation front end we use is an Agilent HPLC systems for applications requiring analytical (0.1-1 ml/min) flow. For nano-flow applications we use a LC Packings/Dionex LC system with 2D capabilities.

Liquid... Show more »

Our main tool for LC-MS services is a Waters/Micromass Quattro triple quadrupole tandem mass spectrometer. The separation front end we use is an Agilent HPLC systems for applications requiring analytical (0.1-1 ml/min) flow. For nano-flow applications we use a LC Packings/Dionex LC system with 2D capabilities.

Liquid Chromatography Mass Spectrometry (LC/MS) is an analytical technique for identification, quantitation and mass analysis of a wide variety of non-volatile or semi-volatile compounds in a mixture. This technique also allows for the structural elucidation of unknown molecules through fragmentation.

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$60.00 USD per Hour
1352600217167 47f5ac1d1c1f1961047f03946640b17d

Lincoln, Nebraska
83% Positive Reviews
11 Orders Completed

Determination of most elements in the Periodic Table is possible, except for most halogens, first row elements and some non-metal elements such as S and As where the analysis is only possible if the concentration is at the mg/L (ppm) and ug/L (ppb) respectively. Instrument is equipped with a collision cell for the removal of... Show more »

Determination of most elements in the Periodic Table is possible, except for most halogens, first row elements and some non-metal elements such as S and As where the analysis is only possible if the concentration is at the mg/L (ppm) and ug/L (ppb) respectively. Instrument is equipped with a collision cell for the removal of polyatomic interferences. A single sample injection requires about 200 uL, although less volume is possible if the sensitivity is not compromised.

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$210.35 USD per Sample
1340747422000

Tampa, Florida
100% Positive Reviews
9 Orders Completed

Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry (LC-MRM) is used to quantify components in complex mixtures. Our laboratory uses this technique for quantification of protein biomarkers. Assay development prior to sample analysis includes synthesis and characterization of standard peptides. Note that multiple proteins can be quantified in each sample.

$244.00 USD per Hour
1352588488858 5e19539007fbb5c1d8afdf0f79a93097

College Station, Texas
100% Positive Reviews
22 Orders Completed

For mass determination or protein identification of one spot, there is a 0.25 hour minimum; ($244 per hour) does not include sample preparation. Pricing varies with project requirements. Please contact the facility.

$280.00 USD per Sample
Tnzgu3hitzy7xjzt7nvv cfg

Rensselaer, New York
100% Positive Reviews
10 Orders Completed

Targeted detection and quantitation, also called multiple reaction monitoring-initiated detection and sequencing (MIDAS). Multiple reaction monitoring (MRM) is used as an extremely sensitive MS survey scan for the targeted peptide from a known protein. This is automatically followed by peptide sequencing confirmation. The method... Show more »

Targeted detection and quantitation, also called multiple reaction monitoring-initiated detection and sequencing (MIDAS). Multiple reaction monitoring (MRM) is used as an extremely sensitive MS survey scan for the targeted peptide from a known protein. This is automatically followed by peptide sequencing confirmation. The method is capable of detecting and sequencing peptides at low femtomole levels with high selectivity. The MIDAS workflow is extremely useful for biomarker validation and cellular analysis particularly when a monoclonal antibody is not available or western blot is inclusive. (Molecular &Cellular Proteomics 4:1134–1144, 2005).

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Price on request
1314398464000

Kinexus Bioinformatics Corporation

Vancouver, British Columbia
100% Positive Reviews
23 Orders Completed

Turnaround time: ~2-8 weeks depending on scope of work

$95.00 USD per Hour
1352510432255 7fffb663120dd173020ed4b9bc223583

St. Louis, Missouri
100% Positive Reviews
9 Orders Completed
Price on request
1352595265361 7fffb663120dd173020ed4b9bc223583

High Throughput Screening Service

Irvine, California
100% Positive Reviews
3 Orders Completed
$85.00 USD per Hour
1355241939756 ea3368b8129fe67a01946f7b3833364f

St. Louis, Missouri
100% Positive Reviews
4 Orders Completed

Inductively coupled plasma mass spectroscopy (ICP-MS)
Academic Service Rate: $85/hr
Industry Service Rate: $200/hour

$120.00 USD per Sample
1331320859000

Ontario, California
100% Positive Reviews
5 Orders Completed

Instrument
HP 5890 GC - HP 5970 MSC