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Mass Spectrometry

Mass spectrometry is used to determine the mass, and thus identity, of a sample molecule. Ionized molecules passing it through a strong magnetic field will have their trajectories deflected by an amount proportional to their mass to charge ratio (m/z). This m/z spectrum can then be... Show more »

Mass spectrometry is used to determine the mass, and thus identity, of a sample molecule. Ionized molecules passing it through a strong magnetic field will have their trajectories deflected by an amount proportional to their mass to charge ratio (m/z). This m/z spectrum can then be used to identify the molecule by comparison to a vast database of known molecular m/z spectra.
To accomplish this, a mass spectrometry experiment is broken down into three basic steps. First, the sample is ionized. (If the sample is already a gas, it can be directly ionized; if it is a liquid or solid it must first be vaporized before ionization.) The ions are next organized into a stream that is guided through the mass analyzer, where they are exposed to magnetic fields that deflect their trajectory based on their mass. This deflection is recorded by the mass detector.
There are many varied ways to ionize your sample. The process of ionizing a molecule can also break that molecule into fragments, in many cases in predictable patterns based on bond strengths and various functional groups that can yield structural information about the sample molecule. Hard ionization methods tend to produce lots of fragmentation, while soft ionization methods result in much less fragmentation. Thus, the m/z spectra will reflect the masses of the ionized sample analyte as well as ionized fragments of that analyte, resulting in a unique “fingerprint” for that molecule.
There are also multiple methods for perturbing the ions’ flight and detecting the ions. Combining different methods of ionization, deflection and detection make mass spectrometry a versatile technique with relevance across a broad range of scientific areas. (Credit: Fraser Tan) « Show less

We found 4 labs offering this service 100% positive ratings across 2 reviews.

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1314398464000

Kinexus Bioinformatics Corporation

Vancouver, British Columbia
100% Positive Reviews
23 Orders Completed

Turnaround time: ~2-8 weeks depending on scope of work

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Mass Spectrometry Facility

Bozeman, Montana
No Reviews Available
Price on request
1315354995000

David H Murdock Research Institute

Kannapolis, North Carolina
No Reviews Available

Analyte and biomarker discovery using LC and GC mass spectrometry-based techniques

Metabolomics and Proteomics mass spectrometry-based research

Biomarker verification and validation studies using MS, NMR or immunoassay technologies

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1383845848739 3e67cba7e4420916c3fad7cfcc75e16c

Proteomics Core

Belfield, Dublin
No Reviews Available

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.


Instrumentation:
In terms of equipment, MSR avails of several mass... Show more »

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.


Instrumentation:
In terms of equipment, MSR avails of several mass spectrometers in order to be able to cover almost all possible experimental workflows in mass spectrometry-based proteomics. These include:

  • Thermo Fisher Q-Exactive
  • Thermo Fisher Orbitrap
  • Thermo Fisher LTQ ion trap
  • Agilent 6520 Quadrupole-ToF
  • Agilent 6460 Triple Quadrupole
  • ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector
  • Bruker HCTUltra II ETD ion trap.

The AB Sciex 4800 MALDI-Tof/Tof mass spectrometer with a CovalX HM2 high mass-range detector is applied to gel-based and off-line liquid chromatography (LC)-based, high throughput MALDI-MS/MS proteomic workflows or, in linear mode, to accurate determination of the mass of intact proteins or protein complexes up to 1.2 Megadaltons.

The Q-Exactive, LTQ-Orbitrap, the ion traps, the quadrupole time-of-flight and a triple quadrupole mass spectrometers cover all possible needs in liquid-chromatography-tandem mass spectrometry.

Furthermore, the MSR offers access to LC-based workflows supporting the multi-dimensional protein identification technology (MudPIT) and a nano-LC system coupled to a MALDI target loading robot to support off-line LC MALDI-MS.

Software suite
Raw data files generated by each mass spectrometer are subjected to database search with the appropriate algorithm (Mascot, TurboSequest, Spectrum Mill) and database.

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