Browse Why Science Exchange How It Works List Your Lab Help Center
Sign Up Login

IHC

Immunohistochemistry

Immunohistochemistry (IHC) is the detection of proteins in fixed (dead) tissue samples using labeled antibodies. Tissue samples are preserved (fixed) to maintain the integrity of the proteins, permeabilized to allow entry of the antibody into the cells, blocked with a solution that... Show more »

Immunohistochemistry (IHC) is the detection of proteins in fixed (dead) tissue samples using labeled antibodies. Tissue samples are preserved (fixed) to maintain the integrity of the proteins, permeabilized to allow entry of the antibody into the cells, blocked with a solution that minimizes incorrect binding of the antibody, and then the proteins of interest are bound by a specific antibody (primary antibody). IHC can be direct, where the protein is bound by a labeled primary antibody, or indirect, where an unlabeled primary antibody is bound by a second labeled antibody (secondary antibody). Common labels are fluorescent, enzymatic, radioactive, or collodial gold. The sample is analyzed through light or fluorescent microscopy. IHC is used to visualize the location of proteins in tissue, identify abnormal cells, and other diagnostics.
(Credit: Brooke Anderson-White) « Show less

Save 93% compared with the cost of performing immunohistochemistry in your lab.

We found 37 labs offering this service with prices from $19.00 to $65.00 per slide. 97% positive ratings across 30 reviews.

Price on request
9jjpx6lpqamir2zoca0w creative diagnostics

Creative Diagnostics

Shirley NY
No Reviews Available

Immunohistochemistry (IHC) refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by using the principle of antibodies binding specifically to antigens in the tissue. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyze a color-producing... Show more »

Immunohistochemistry (IHC) refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by using the principle of antibodies binding specifically to antigens in the tissue. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyze a color-producing reaction.

Creative Diagnostics has been providing high quality IHC service to our customers for years. We have established well-tested procedure to meet your research demands, which include different combinations of various tissue fixation processes, antigen retrieval, antibody titration options and eliminate background staining. Also, we have unparalleled bioinformatics support in performing IHC studies.

Creative Diagnostics offers a variety of IHC services:

Diagnostic IHC - For our veterinary diagnostic services, we offer a full complement of IHC markers to phenotype lymphomas and differentiate various soft tissue tumors.

Research IHC - We provide to screen the most appropriate antibody from our existing optimized antibodies. Besides, we are able to maximize the staining signals even for antigens with extremely low expression level using our proprietary technology.

Combination IHC and ISH - This service allow you to quickly visualize your target protein and transcript in its native setting.

« Show less
$19.00 USD per Slide
Bl7lyzq9spcwcrqhjgnn georgetownlogo

Washington District of Columbia
100% Positive Reviews
34 Orders Completed

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and... Show more »

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and specificity.

Deliverables:
- Publication quality immuno-stained slides
- Optimized staining protocols for established and novel antibodies

Turn around time:
For Established antibodies - 1-2 weeks
For Novel antibodies - 1 month

« Show less
Price on request
1343083028000

Reveal Biosciences

San Diego California
100% Positive Reviews
16 Orders Completed

This service includes the IHC staining of paraffin (FFPE) or frozen tissue sections. Tissue preparation including wax processing, embedding and sectioning (microtomy), or frozen section preparation (cryotomy) are also available on request. In addition we offer digital slide scanning and biomarker quantification resulting in... Show more »

This service includes the IHC staining of paraffin (FFPE) or frozen tissue sections. Tissue preparation including wax processing, embedding and sectioning (microtomy), or frozen section preparation (cryotomy) are also available on request. In addition we offer digital slide scanning and biomarker quantification resulting in quantitative IHC data. Assay development and optimization using colorimetric, fluorescent and multiplex approaches are available. Optimization of your choice of antibody and detection system is also available on request.

Our state-of-the-art Leica Bond immunostainer produces consistent quality high throughput immunohistochemistry (IHC). We have developed and validated a wide range of automated IHC protocols for rodent and human tissue. Examples include Ki67, PCNA, caspase-3, E-cadherin, CD3, CD8, and many more. New antibodies can also be optimized on request.

« Show less
$575.00 USD per Unit
Arttamxisnsw810b1xi6 georgetownlogo

Washington District of Columbia
100% Positive Reviews
16 Orders Completed
1 Endorsement

Whole-mount immunohistochemistry
Whole-mount in immunohistochemistry of zebrafish embryos. Colorimetric or fluorescent detection is available. Please contact us to discuss experimental details.
$575. (per antibody)

$22.50 USD per Slide
1352517118347 431a0fe323589e11291b277f08c0f006

New York New York
100% Positive Reviews
9 Orders Completed

Base prices are for External Academic Institutions. Pricing is subject to change

Chromogenic:
- Antibody Verification - Verifying antibody staining conditions for in-house antibodies that have not been used for a period of 1 month or more (sectioning service fee not included) $10.50/slide

Base prices are for External Academic Institutions. Pricing is subject to change

Chromogenic:
- Antibody Verification - Verifying antibody staining conditions for in-house antibodies that have not been used for a period of 1 month or more (sectioning service fee not included) $10.50/slide

  • Manual method - Non-automated, standard 2 step indirect (limited to 20 slides at a time per staining run) $45.00/slide

  • Antibody Optimization - Optimizing new or untested antibodies (use of in house TMA sectioning service fee not included) $30.00/slide

  • Double Label - 2 Color sequential double stains (requires prior optimization of both markers) $37.50/slide

  • Routine Automated - For markers with established conditions $22.50/slide

Fluorescent:
- Antibody Optimization - Single color with counter $18.75/slide

  • Antibody Routine- Single color with counter $13.88/slide
« Show less
$27.50 USD per Sample
1359607304737 9f360c5ab7736510df54c882e9dbf188

Gainesville Florida
100% Positive Reviews
8 Orders Completed

The Immunohistochemistry (IHC) lab provides in-dept consultations including antibody selection, training, and services for established assays as well as testing new antibodies.

Includes primary antibodies when in stock. Per section prices.

Price on request
1359394176163 b2813ee3587f6568c12ed9079f54fb41

Confocal Imaging Core (BIDMC)

Boston Massachusetts
100% Positive Reviews
7 Orders Completed

Immunostaining (frozen and paraffin embedded tissues):
Immunofluorescence staining
Immunoperoxidase staining

Image Processing (Volocity software):
Quantification
Visualization

Staining IPOX- Frozen
BIDMC Price $305.00
External Academic $457.50
External Corporate $610.00

Immunofluorescence-Frozen
BIDMC $... Show more »

Immunostaining (frozen and paraffin embedded tissues):
Immunofluorescence staining
Immunoperoxidase staining

Image Processing (Volocity software):
Quantification
Visualization

Staining IPOX- Frozen
BIDMC Price $305.00
External Academic $457.50
External Corporate $610.00

Immunofluorescence-Frozen
BIDMC $ 210.00
EA $ 315.00
EC $ 420.00

Immunofluorescence-Frozen (TUNEL)
BICMC $ 470.00
EA $ 705.00
EC $ 940.00

Staining IPOX- Paraffin
BIDMC Price $390.00
EA $585.00
EC $780.00

Immunofluorescence- Paraffin
BIDMC $ 250.00
EA $ 375.00
EC $ 500.00

Staining IPOX -Paraffin (TUNEL)
BIDMC $ 530.00
EA $ 795.00
EC $ 1,060.00

Trilogy Charge-back (per run)
BIDMC Price $8.50
EA $10.63
EC $12.75

Equipment:
Zeiss LSM510 Upright Confocal System
Zeiss LSM 510 Inverted Live-cell Confocal System
Zeiss Axioimager M1 Epifluorescence and Brightfield microscope
Dell workstation with Volocity and LSM510 software

« Show less
$65.00 USD per Slide
1352597188388 f297e6586297ff4df474d6fdf73f790c

Athens Georgia
100% Positive Reviews
5 Orders Completed
  • Immunohistochemical stains for infectious agents
  • Immunohistochemical stains for tumor markers/receptors/signaling molecules
    Instrumentation: Dako Autostainer Plus immunohistochemical stainer
    Fees:
    Immunohistochemistry optimization for new antibody (procedure, interpretation, and report)
    per Antibody $400.00 (turn around... Show more »
  • Immunohistochemical stains for infectious agents
  • Immunohistochemical stains for tumor markers/receptors/signaling molecules
    Instrumentation: Dako Autostainer Plus immunohistochemical stainer
    Fees:
    Immunohistochemistry optimization for new antibody (procedure, interpretation, and report)
    per Antibody $400.00 (turn around time: 14 business days)
    Immunohistochemistry with antibodies in use in CPL (inquire about antibody list)
    per Glass slide $65.00 (turn-around time: 7 business days)
    Deliverables: Immunostained slides, detailed report (methods, results, images)
« Show less
Price on request
1346387054000

Translational Pathology Core Laboratory

Los Angeles California
100% Positive Reviews
5 Orders Completed

Services include immunostaining of established antibodies as well as optimization of new antibodies and double staining for brightfield; TUNEL assay for apoptosis is also performed. We have a large library of established antibodies available for use on human and animal tissues [IHC Core Antibodies]. Several chromogens (DAB, Fast... Show more »

Services include immunostaining of established antibodies as well as optimization of new antibodies and double staining for brightfield; TUNEL assay for apoptosis is also performed. We have a large library of established antibodies available for use on human and animal tissues [IHC Core Antibodies]. Several chromogens (DAB, Fast Red, AP Blue, AP Green) and counterstains (hematoxylin, methyl-green, nuclear fast red) are available. The detection system most frequently used is the HP system using DAB with hematoxylin counterstain.

« Show less
$20.00 USD per Slide
1352599923851 654776d09b4b7ca33ea188ae988183f7

Omaha Nebraska
100% Positive Reviews
3 Orders Completed

Per slide, from existing antibody offerings $20.00
Two color, per slide $28.50

Deliverables:
- stained slide
- digital image (optional)