Immunohistochemistry

Immunohistochemistry (IHC) is the detection of proteins in fixed (dead) tissue samples using labeled antibodies. Tissue samples are preserved (fixed) to maintain the integrity of the proteins, permeabilized to allow entry of the antibody into the cells, blocked with a solution that... Show more »

Immunohistochemistry (IHC) is the detection of proteins in fixed (dead) tissue samples using labeled antibodies. Tissue samples are preserved (fixed) to maintain the integrity of the proteins, permeabilized to allow entry of the antibody into the cells, blocked with a solution that minimizes incorrect binding of the antibody, and then the proteins of interest are bound by a specific antibody (primary antibody). IHC can be direct, where the protein is bound by a labeled primary antibody, or indirect, where an unlabeled primary antibody is bound by a second labeled antibody (secondary antibody). Common labels are fluorescent, enzymatic, radioactive, or collodial gold. The sample is analyzed through light or fluorescent microscopy. IHC is used to visualize the location of proteins in tissue, identify abnormal cells, and other diagnostics.
(Credit: Brooke Anderson-White) « Show less

Save 93% compared with the cost of performing immunohistochemistry in your lab.

We found 29 labs offering this service with prices from $19.00 to $55.00 per slide. 96% positive ratings across 24 reviews.

Price on request
1359394176163 b2813ee3587f6568c12ed9079f54fb41

Confocal Imaging Core (BIDMC)

Boston, Massachusetts
100% Positive Reviews
6 Orders Completed

Immunostaining (frozen and paraffin embedded tissues):
Immunofluorescence staining
Immunoperoxidase staining

Image Processing (Volocity software):
Quantification
Visualization

Staining IPOX- Frozen
BIDMC Price $305.00
External Academic $457.50
External Corporate $610.00

Immunofluorescence-Frozen
BIDMC $... Show more »

Immunostaining (frozen and paraffin embedded tissues):
Immunofluorescence staining
Immunoperoxidase staining

Image Processing (Volocity software):
Quantification
Visualization

Staining IPOX- Frozen
BIDMC Price $305.00
External Academic $457.50
External Corporate $610.00

Immunofluorescence-Frozen
BIDMC $ 210.00
EA $ 315.00
EC $ 420.00

Immunofluorescence-Frozen (TUNEL)
BICMC $ 470.00
EA $ 705.00
EC $ 940.00

Staining IPOX- Paraffin
BIDMC Price $390.00
EA $585.00
EC $780.00

Immunofluorescence- Paraffin
BIDMC $ 250.00
EA $ 375.00
EC $ 500.00

Staining IPOX -Paraffin (TUNEL)
BIDMC $ 530.00
EA $ 795.00
EC $ 1,060.00

Trilogy Charge-back (per run)
BIDMC Price $8.50
EA $10.63
EC $12.75

Equipment:
Zeiss LSM510 Upright Confocal System
Zeiss LSM 510 Inverted Live-cell Confocal System
Zeiss Axioimager M1 Epifluorescence and Brightfield microscope
Dell workstation with Volocity and LSM510 software

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$19.00 USD per Slide
1352512079571 5e23ab92baafa44803861d9b82b6346d

Washington, District of Columbia
100% Positive Reviews
22 Orders Completed

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and... Show more »

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and specificity.

Deliverables:
- Publication quality immuno-stained slides
- Optimized staining protocols for established and novel antibodies

Turn around time:
For Established antibodies - 1-2 weeks
For Novel antibodies - 1 month

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Price on request
1343083028000

Reveal Biosciences

San Diego, California
100% Positive Reviews
14 Orders Completed

This service includes the IHC staining of paraffin (FFPE) or frozen tissue sections. Tissue preparation including wax processing, embedding and sectioning (microtomy), or frozen section preparation (cryotomy) are also available on request. In addition we offer digital slide scanning and biomarker quantification resulting in... Show more »

This service includes the IHC staining of paraffin (FFPE) or frozen tissue sections. Tissue preparation including wax processing, embedding and sectioning (microtomy), or frozen section preparation (cryotomy) are also available on request. In addition we offer digital slide scanning and biomarker quantification resulting in quantitative IHC data. Assay development and optimization using colorimetric, fluorescent and multiplex approaches are available. Optimization of your choice of antibody and detection system is also available on request.

Our state-of-the-art Leica Bond immunostainer produces consistent quality high throughput immunohistochemistry (IHC). We have developed and validated a wide range of automated IHC protocols for rodent and human tissue. Examples include Ki67, PCNA, caspase-3, E-cadherin, CD3, CD8, and many more. New antibodies can also be optimized on request.

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$27.50 USD per Sample
1359607304737 9f360c5ab7736510df54c882e9dbf188

Gainesville, Florida
100% Positive Reviews
7 Orders Completed

The Immunohistochemistry (IHC) lab provides in-dept consultations including antibody selection, training, and services for established assays as well as testing new antibodies.

Includes primary antibodies when in stock. Per section prices.

$22.50 USD per Slide
1352517118347 431a0fe323589e11291b277f08c0f006

New York, New York
100% Positive Reviews
7 Orders Completed

Base prices are for External Academic Institutions. Pricing is subject to change

Chromogenic:
- Antibody Verification - Verifying antibody staining conditions for in-house antibodies that have not been used for a period of 1 month or more (sectioning service fee not included) $10.50/slide

Base prices are for External Academic Institutions. Pricing is subject to change

Chromogenic:
- Antibody Verification - Verifying antibody staining conditions for in-house antibodies that have not been used for a period of 1 month or more (sectioning service fee not included) $10.50/slide

  • Manual method - Non-automated, standard 2 step indirect (limited to 20 slides at a time per staining run) $45.00/slide

  • Antibody Optimization - Optimizing new or untested antibodies (use of in house TMA sectioning service fee not included) $30.00/slide

  • Double Label - 2 Color sequential double stains (requires prior optimization of both markers) $37.50/slide

  • Routine Automated - For markers with established conditions $22.50/slide

Fluorescent:
- Antibody Optimization - Single color with counter $18.75/slide

  • Antibody Routine- Single color with counter $13.88/slide
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$575.00 USD per Unit
1406831936761 615b7caac18b466276dce5efc7468027

Washington, District of Columbia
100% Positive Reviews
6 Orders Completed
1 Endorsement

Whole-mount immunohistochemistry
Whole-mount in immunohistochemistry of zebrafish embryos. Colorimetric or fluorescent detection is available. Please contact us to discuss experimental details.
$575. (per antibody)

$40.00 USD per Slide
1352597188388 f297e6586297ff4df474d6fdf73f790c

Athens, Georgia
100% Positive Reviews
3 Orders Completed
  • Immunohistochemical stains for infectious agents
  • Immunohistochemical stains for tumor markers

Fees:

Immunohistochemistry optimization for new antibody (procedure, interpretation, and report)
per Antibody $250.00

Immunohistochemistry with antibodies in use in CPL (inquire about antibody list)
per Glass slide $40.00

Price on request
1371671800497 f5a6754381f53b7eae481cc45da16c69

CaresBio Laboratory

Shelton, Connecticut
67% Positive Reviews
6 Orders Completed

CBL provides contract histology, pathological and immunostaining that includes IHC, and analyses services
Immunostaining (Tissue and Cells).

Deliverables:
- images
- image analysis data (if requested analysis)
- presented in Microsoft power point until unless requested by client in any other method

$20.00 USD per Slide
1352599923851 654776d09b4b7ca33ea188ae988183f7

Omaha, Nebraska
100% Positive Reviews
1 Order Completed

Per slide, from existing antibody offerings $20.00
Two color, per slide $28.50

Deliverables:
- stained slide
- digital image (optional)

Price on request
1360106456895 60855c4e1b5c77d8c28c8f6ec0f458e9

Pathology Diagnostics

Waterbeach, Cambridge
6 Orders Completed

We have extensive experience of IHC assay validation on a wide variety of automated IHC staining platforms, IHC staining and diagnostic evaluation from semi-automated to fully computerised automated image analysis on digital scanned images of whole tissue sections. Our team of board-certified pathologists is available to provide... Show more »

We have extensive experience of IHC assay validation on a wide variety of automated IHC staining platforms, IHC staining and diagnostic evaluation from semi-automated to fully computerised automated image analysis on digital scanned images of whole tissue sections. Our team of board-certified pathologists is available to provide on-site or remote review of biomarker test data as required by regulatory authorities to deliver accurate quality-controlled client data on time and within budget to enable you to make reliable data-driven decisions.

Over the last few years we have performed over 75,000 analyses of biomarkers on IHC stained sections of patient samples from a wide variety of normal and diseased tissues in the following therapy areas:
- oncology
- cardiovascular
- bone and joint disease
- metabolic disorders
- gastrointestinal disease
We have evaluated over 130 protein biomarkers in human and rodent tissues in the following areas:
- cell surface receptors
- proliferation/apoptosis
- DNA damage sensing & repair
- signal transduction
- immune system
- vascularisation
- metabolic
- clinical diagnostic markers

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