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IHC

Immunohistochemistry

Immunohistochemistry (IHC) is the detection of proteins in fixed (dead) tissue samples using labeled antibodies. Tissue samples are preserved (fixed) to maintain the integrity of the proteins, permeabilized to allow entry of the antibody into the cells, blocked with a solution that... Show more »

Immunohistochemistry (IHC) is the detection of proteins in fixed (dead) tissue samples using labeled antibodies. Tissue samples are preserved (fixed) to maintain the integrity of the proteins, permeabilized to allow entry of the antibody into the cells, blocked with a solution that minimizes incorrect binding of the antibody, and then the proteins of interest are bound by a specific antibody (primary antibody). IHC can be direct, where the protein is bound by a labeled primary antibody, or indirect, where an unlabeled primary antibody is bound by a second labeled antibody (secondary antibody). Common labels are fluorescent, enzymatic, radioactive, or collodial gold. The sample is analyzed through light or fluorescent microscopy. IHC is used to visualize the location of proteins in tissue, identify abnormal cells, and other diagnostics.
(Credit: Brooke Anderson-White) « Show less

Save 93% compared with the cost of performing immunohistochemistry in your lab.

We found 33 labs offering this service with prices from $19.00 to $65.00 per slide. 96% positive ratings across 26 reviews.

$19.00 USD per Slide
Bl7lyzq9spcwcrqhjgnn georgetownlogo

Washington, DC
100% Positive Reviews
31 Orders Completed

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and... Show more »

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and specificity.

Deliverables:
- Publication quality immuno-stained slides
- Optimized staining protocols for established and novel antibodies

Turn around time:
For Established antibodies - 1-2 weeks
For Novel antibodies - 1 month

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$27.50 USD per Sample
1359607304737 9f360c5ab7736510df54c882e9dbf188

Gainesville, Florida
100% Positive Reviews
8 Orders Completed

The Immunohistochemistry (IHC) lab provides in-dept consultations including antibody selection, training, and services for established assays as well as testing new antibodies.

Includes primary antibodies when in stock. Per section prices.

$20.00 USD per Slide
1352599923851 654776d09b4b7ca33ea188ae988183f7

Omaha, Nebraska
100% Positive Reviews
3 Orders Completed

Per slide, from existing antibody offerings $20.00
Two color, per slide $28.50

Deliverables:
- stained slide
- digital image (optional)

$65.00 USD per Slide
1352597188388 f297e6586297ff4df474d6fdf73f790c

Athens, Georgia
100% Positive Reviews
5 Orders Completed
  • Immunohistochemical stains for infectious agents
  • Immunohistochemical stains for tumor markers/receptors/signaling molecules
    Instrumentation: Dako Autostainer Plus immunohistochemical stainer
    Fees:
    Immunohistochemistry optimization for new antibody (procedure, interpretation, and report)
    per Antibody $400.00 (turn around... Show more »
  • Immunohistochemical stains for infectious agents
  • Immunohistochemical stains for tumor markers/receptors/signaling molecules
    Instrumentation: Dako Autostainer Plus immunohistochemical stainer
    Fees:
    Immunohistochemistry optimization for new antibody (procedure, interpretation, and report)
    per Antibody $400.00 (turn around time: 14 business days)
    Immunohistochemistry with antibodies in use in CPL (inquire about antibody list)
    per Glass slide $65.00 (turn-around time: 7 business days)
    Deliverables: Immunostained slides, detailed report (methods, results, images)
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Price on request
1333585564000

Smart Assays

Nes Ziyyona
No Reviews Available

Standard marker antibodies (Ki67, PCNA, cytokeratins etc.)

Validation of polyclonal and monoclonal antibodies on sections of normal and pathological tissues

Price on request
1353108450158 25fbec7806de5e29707d9ef0d93a893c

Cureline

South San Francisco, California
No Reviews Available

Cureline has great level of expertise in providing IHC services to support your research in oncology, inflammation and metabolic diseases. FFPE or frozen specimens are strictly selected for quality and fully annotated with clinical information.

Many immunostains are routinely available along with control tissues (provided at... Show more »

Cureline has great level of expertise in providing IHC services to support your research in oncology, inflammation and metabolic diseases. FFPE or frozen specimens are strictly selected for quality and fully annotated with clinical information.

Many immunostains are routinely available along with control tissues (provided at no additional charges). The stock of commonly used reagents including primary antibody (additional charges apply or may be provided by the client) and secondary reagents (included in price) is maintained by the laboratory director and list of these reagents is available upon request. Reagents that are not on the list and unusually expensive reagents must be provided by the client.

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Price on request
1343083028000

Reveal Biosciences

San Diego, California
100% Positive Reviews
14 Orders Completed

This service includes the IHC staining of paraffin (FFPE) or frozen tissue sections. Tissue preparation including wax processing, embedding and sectioning (microtomy), or frozen section preparation (cryotomy) are also available on request. In addition we offer digital slide scanning and biomarker quantification resulting in... Show more »

This service includes the IHC staining of paraffin (FFPE) or frozen tissue sections. Tissue preparation including wax processing, embedding and sectioning (microtomy), or frozen section preparation (cryotomy) are also available on request. In addition we offer digital slide scanning and biomarker quantification resulting in quantitative IHC data. Assay development and optimization using colorimetric, fluorescent and multiplex approaches are available. Optimization of your choice of antibody and detection system is also available on request.

Our state-of-the-art Leica Bond immunostainer produces consistent quality high throughput immunohistochemistry (IHC). We have developed and validated a wide range of automated IHC protocols for rodent and human tissue. Examples include Ki67, PCNA, caspase-3, E-cadherin, CD3, CD8, and many more. New antibodies can also be optimized on request.

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$1,084.00 USD per Cell Line
1352596202298 7b2631812fe02a4accae69531c8df8ba

Aurora, Colorado
No Reviews Available

Pluripotency analysis via Immunocytochemistry Staining
Immunocytochemistry staining for pluripotency markers (Oct 4, Nanog, TRA-160, SSEA3, SSEA40)

Price on request
1346387054000

Translational Pathology Core Laboratory

Los Angeles, California
2 Orders Completed

Services include immunostaining of established antibodies as well as optimization of new antibodies and double staining for brightfield; TUNEL assay for apoptosis is also performed. We have a large library of established antibodies available for use on human and animal tissues [IHC Core Antibodies]. Several chromogens (DAB, Fast... Show more »

Services include immunostaining of established antibodies as well as optimization of new antibodies and double staining for brightfield; TUNEL assay for apoptosis is also performed. We have a large library of established antibodies available for use on human and animal tissues [IHC Core Antibodies]. Several chromogens (DAB, Fast Red, AP Blue, AP Green) and counterstains (hematoxylin, methyl-green, nuclear fast red) are available. The detection system most frequently used is the HP system using DAB with hematoxylin counterstain.

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Price on request
1359394176163 b2813ee3587f6568c12ed9079f54fb41

Confocal Imaging Core (BIDMC)

Boston, Massachusetts
100% Positive Reviews
7 Orders Completed

Immunostaining (frozen and paraffin embedded tissues):
Immunofluorescence staining
Immunoperoxidase staining

Image Processing (Volocity software):
Quantification
Visualization

Staining IPOX- Frozen
BIDMC Price $305.00
External Academic $457.50
External Corporate $610.00

Immunofluorescence-Frozen
BIDMC $... Show more »

Immunostaining (frozen and paraffin embedded tissues):
Immunofluorescence staining
Immunoperoxidase staining

Image Processing (Volocity software):
Quantification
Visualization

Staining IPOX- Frozen
BIDMC Price $305.00
External Academic $457.50
External Corporate $610.00

Immunofluorescence-Frozen
BIDMC $ 210.00
EA $ 315.00
EC $ 420.00

Immunofluorescence-Frozen (TUNEL)
BICMC $ 470.00
EA $ 705.00
EC $ 940.00

Staining IPOX- Paraffin
BIDMC Price $390.00
EA $585.00
EC $780.00

Immunofluorescence- Paraffin
BIDMC $ 250.00
EA $ 375.00
EC $ 500.00

Staining IPOX -Paraffin (TUNEL)
BIDMC $ 530.00
EA $ 795.00
EC $ 1,060.00

Trilogy Charge-back (per run)
BIDMC Price $8.50
EA $10.63
EC $12.75

Equipment:
Zeiss LSM510 Upright Confocal System
Zeiss LSM 510 Inverted Live-cell Confocal System
Zeiss Axioimager M1 Epifluorescence and Brightfield microscope
Dell workstation with Volocity and LSM510 software

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