ELISA

Enzyme-linked immunosorbent assay

Enzyme-linked immunosorbent assay (ELISA) is an affinity-based method, usually carried out in multi-well plates, to determine if an antigen, often a protein or antibody, is present in a sample. The general method for ELISA begins with the immobilization of the antigen in a... Show more »

Enzyme-linked immunosorbent assay (ELISA) is an affinity-based method, usually carried out in multi-well plates, to determine if an antigen, often a protein or antibody, is present in a sample. The general method for ELISA begins with the immobilization of the antigen in a multi-well plate. Either the whole sample can be allowed to settle and adhere to the plate or an antibody specific to the antigen can be bound to the plate which then captures and immobilizes the antigen. The antigen is next detected either directly, when the antigen is bound by an enzyme-labeled primary antibody, or indirectly, when the bound unlabeled primary antibody is then bound by a second enzyme-labeled antibody (secondary antibody). After binding, the enzyme substrate is added, usually resulting in a color change, weak radioactivity, fluorescence, or chemiluminescence that can be detected and measured. ELISA is a common technique with uses including as a diagnostic for infections and diseases like HIV, as a toxicology tool to screen for drugs, and as a method for detection of allergens in the food industry. (Credit: Brooke Anderson-White) « Show less

Save 16% compared with the cost of performing ELISAs in your lab.

We found 94 labs offering this service with prices from $149.00 to $650.00 per plate. 97% positive ratings across 67 reviews.

Starting at $195.00 USD per plate
1371590680511 a2c33e6508cb526c0b1970ac29d00e98

San Antonio Texas
80 Orders Completed
100% Positive

Capabilities:
- Kit-based (provided by the researcher), custom designed ELISA
- Indirect, Sandwich, competitive
- Homogenization/Sonication of cell/tissue lysates
- Transfection/Transduction of mammalian/non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
-... Show more »

Capabilities:
- Kit-based (provided by the researcher), custom designed ELISA
- Indirect, Sandwich, competitive
- Homogenization/Sonication of cell/tissue lysates
- Transfection/Transduction of mammalian/non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze chromogenic substrates

Equipment:
- Multi-channel and automatic pipets

Number of samples processed in a single experiment: 96-well plate format

Typical turn-around time: 14 days

ELISA with TMB substrate. Primary Antibody, standards or ELISA kit needs to be provided.

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Starting at $218.00 USD per plate
1314212146000

Woodbridge Connecticut
73 Orders Completed
100% Positive

In collaboration with our affiliate NorthEast Bioanalytical Laboratories, we provide services under GLP and FDA guidelines for:

• ELISA based assays
• Translational medicine assays

$218 per ELISA with a minimum of 4 ELISA's assayed at a time.

Capabilities:
- Kit-based, custom designed ELISA
- Sandwich,... Show more »

In collaboration with our affiliate NorthEast Bioanalytical Laboratories, we provide services under GLP and FDA guidelines for:

• ELISA based assays
• Translational medicine assays

$218 per ELISA with a minimum of 4 ELISA's assayed at a time.

Capabilities:
- Kit-based, custom designed ELISA
- Sandwich, competitive, in-Cell ELISA (ICE)
- Homogenization of cell/tissue lysates
- Transfection/Transduction of mammalian/non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates

Equipment:
- SpectraMax® M5e
- Multi-channel pipets

Number of samples processed in a single experiment: 72

Typical turn-around time: 5 days

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Starting at $300.00 USD per plate
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Raleigh North Carolina
57 Orders Completed
100% Positive

Capabilities:
- Kit-based, custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization of cell/tissue lysates
- Transfection/Transduction of mammalian cells
- Analyze serum, plasma, cells, tissues
- In-house mouse/rat serum and tissues
- Analyze chromogenic substrates

Equipment:
- Bio-Rad microplate... Show more »

Capabilities:
- Kit-based, custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization of cell/tissue lysates
- Transfection/Transduction of mammalian cells
- Analyze serum, plasma, cells, tissues
- In-house mouse/rat serum and tissues
- Analyze chromogenic substrates

Equipment:
- Bio-Rad microplate reader
- Multi-channel pipets

Number of samples processed in a single experiment: 96-well plate

Typical turn-around time: 5-10 business days

ELISA assay development, validation and sample processing

Over 100 established ELISA assays for human, mouse and rat proteins, including: interleukins, interferons, TGFs, EGF, etc.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

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Starting at $650.00 USD per plate
1379046072341 d4243b2d6ca86e479e25a1746e1ac940

Mountain View California
39 Orders Completed
100% Positive
2 Endorsements

ELISA will be performed using TMB substrate. Primary Antibody and standards provided by customer.

Starting at $350.00 USD per plate
1346427153000

Elmsford New York
23 Orders Completed
100% Positive
1 Endorsement

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze... Show more »

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates

Equipment:
- Multi-channel pipets
- Automatic pipets
- Plate washers

Number of samples processed in a single experiment: In duplicate, 96

Typical turn-around time: 5 days

Troubleshooting protocol: test negative and positive controls, select optimal dilutions for spikes, optimize time and temperature of incubation (all if necessary)

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Price on request
Rpibmvr5tpgbu0hywshq karebay

KareBay Biochem Inc.

Monmouth Junction New Jersey
17 Orders Completed
100% Positive

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique used to detect the presence of a specific antigen or antibody in a sample. The ELISA has maintained its popularity for over 40 years due to its high specificity, sensitivity, and reliability – characteristics that customers consistently cite for using the... Show more »

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique used to detect the presence of a specific antigen or antibody in a sample. The ELISA has maintained its popularity for over 40 years due to its high specificity, sensitivity, and reliability – characteristics that customers consistently cite for using the method.

ELISA Services and Test Methods

Direct ELISA

The direct version of the ELISA assay uses monoclonal antibodies to test for a specific antigen. This form of ELISA testing is used primarily in the immunohistochemical staining of tissues and cells.

Advantages

Direct ELISA, when compared to other forms of ELISA testing, is performed quickly because only one antibody is employed. This assay can be used to test specific antibody-to-antigen reactions, and helps avoid complications due to cross-reactivity between other antibodies. Disadvantages The primary antibody must be labeled individually, which can be time-consuming and arduous when performing multiple experiments. Also, the signal is not as amplified in direct ELISA, compared with the indirect approach, which can be a disadvantage in some applications involving trace analyte detection.

Indirect ELISA Test

The indirect detection method uses a labeled secondary antibody for detection and is the most popular format for ELISA. The secondary antibody has specificity for the primary antibody. In a sandwich ELISA, it is critical that the secondary antibody is specific for the detection of the primary antibody only (and not the capture antibody) or the assay will not be specific for the antigen. Generally, this is achieved by using capture and primary antibodies from different host species (e.g., mouse IgG and rabbit IgG, respectively). For sandwich assays, it is beneficial to use secondary antibodies that have been cross-adsorbed to remove any antibodies that have affinity for the capture antibody. 
The most powerful ELISA assay format is the sandwich assay. This type of capture assay is called a “sandwich” assay because the analyte to be measured is bound between two primary antibodies – the capture antibody and the detection antibody. The sandwich format is used because it is sensitive and robust.

Competitive ELISA Test

The competitive ELISA is used to quantify antigen using a competition method. Briefly, the free antigen and antibody are incubated to form antigen-antibody complex and then the complex is added to an antigen-coated surface in the assay plate. The unbound antibody-antigen complex is washed off before adding enzyme-linked secondary antibody against the primary antibody. The substrate is then added and the antigen concentration is subsequently determined by the signal strength elicited by the enzyme-substrate reaction. In this assay, the enzyme-linked secondary antibody competes with the sample antigen, which is associated with the primary antibody.

Our Service

With a professional ELISA team and state-of-the-art equipment, KareBay™ Biochem will provide you with the highest quality service. It is simple to work with us. You send us your samples, we send you back the test results.

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Price on request
1389324893793 59c52691750c179e34cc162d6ccb96e0

ADS Biosystems Inc.

San Diego California
11 Orders Completed
100% Positive
Price on request
1371671800497 f5a6754381f53b7eae481cc45da16c69

CaresBio Laboratory

Shelton Connecticut
9 Orders Completed
100% Positive
Price on request
1396291727675 7f9ffa6a3f1334adc3a796fb805f3db7

NANOBIOTEC, LLC

Hanover New Jersey
9 Orders Completed
100% Positive

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed ELISA
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we... Show more »

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed ELISA
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we measure cytokines /transcription factors /protein phosphorylation/histone acetylation, etc
• Number of samples processed in a single experiment: duplicate or triplicate, 96-well plate format

Equipment:

• Multi-channel pipets
• Automatic pipets
• Automatic Plate washers
• Tecan Plate Reader

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding 96-well plate following a standard assay design layout and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed, 50-200 ul depending on duplicate or triplicate.
• Please ship samples in dry ice.

Typical turn-around time:
• 1-2 weeks or sooner, upon receiving all materials

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Starting at $200.00 USD per plate
Hngwdueq1e7idpiqe4g0 novatein

Woburn Massachusetts
6 Orders Completed
100% Positive

As a manufacturer of reliable ELISA kits, our scientists are experts in performing and troubleshooting different types of ELISAs. We use good laboratory practices, to perform our assays either for assay validation or quality control.

Customers have the flexibility to choose our in-house manufactured ELISA kit, in which case we... Show more »

As a manufacturer of reliable ELISA kits, our scientists are experts in performing and troubleshooting different types of ELISAs. We use good laboratory practices, to perform our assays either for assay validation or quality control.

Customers have the flexibility to choose our in-house manufactured ELISA kit, in which case we offer 10%-25% discount on the kit price.

Resources:

  • Ph.D. level scientists experienced in customer support
  • Spectramax 384plus plate reader
  • ELISA plate washer
  • Multichannel pipettes (electronic and manual)
  • Multiplex assays to read luminescence

Capabilities:

  • Sandwich, competitive, in-cell ELISAs
  • Analyze serum, plasma, urine, cell and tissue lysates
  • Screen sandwich pair and optimize sandwich assays
  • Perform kit-based or custom designed ELISA
  • Perform Immuno-PCR based ultra-sensitive detection assays
  • Assay validation/ protocol optimization for commercial kits (if it is not manufactured by us)
  • Analyze multiple substrates

Number of samples processed in a single experiment: 100

Typical turn-around time: 3-5 business days depending on the sample volume (for the kit in stock or provided by the customer)

Price:

$200 per 96-well plate (if the kit is purchased from us)
$250 per 96-well plate (if the kit is purchased from outside)
1 96-well plate minimum (multiples of 96 well plates)

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