16S rRNA sequencing

We found 23 labs offering this service with prices from $18.00 to $159.16 per sample. 100% positive ratings across 64 reviews.

Price on request
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Quick Biology

Pasadena CA
83 Orders Completed
100% Positive
9 Endorsements

Please inquire for a quote

Starting at $50.00 USD per library
1357181560783 14f99b1ec8d88e3021c9ba8b08273f2a

Fremont California
43 Orders Completed
100% Positive

16S sequencing enables unparalleled insight of microbial communities, enabling researchers to study a variety of microbiomes such as soil and intestinal flora. SeqMatic offers sequencing and analysis of 16S rRNA gene for bacterial communities. Dozens to hundreds samples can be sequenced and analyzed with rapid turnaround... Show more »

16S sequencing enables unparalleled insight of microbial communities, enabling researchers to study a variety of microbiomes such as soil and intestinal flora. SeqMatic offers sequencing and analysis of 16S rRNA gene for bacterial communities. Dozens to hundreds samples can be sequenced and analyzed with rapid turnaround times.

Sequencing is performed on the Illumina MiSeq instrument, which is the best choice for 16S sequencing due to its ability to generate high quality data from PCR amplicons.

Free data analysis is included in the form of a summary table showing the identified microbial species and it's abundance. Additional bioinformatics services are also available for an additional fee.

Please request an estimate with your total number of samples for full pricing details.

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Starting at $100.00 USD per sample
Oevbiu4psssizskmuh2e admera

South Plainfield NJ
29 Orders Completed
100% Positive

Service description
The goal of 16S Ribosomal RNA Sequencing is to determine the type and relative abundance of bacterial and archaeal species in heterogeneous samples, such as soil, marine, or gut microbiome. Floracheck™ is a proprietary assay that improves upon current 16S metagenomics techniques with significant sensitivity... Show more »

Service description
The goal of 16S Ribosomal RNA Sequencing is to determine the type and relative abundance of bacterial and archaeal species in heterogeneous samples, such as soil, marine, or gut microbiome. Floracheck™ is a proprietary assay that improves upon current 16S metagenomics techniques with significant sensitivity and specificity. Side-by-side comparison with the most commonly used 16S metagenomics assays reveals that Floracheck™ has the ability to detect more bacterial and archaeal genera with a lower limit of detection for both environmental and mammalian species.

Advantages:
• Higher sensitivity
• Low bias
• Cost-effective
• Fast turnaround

Region coverage:
Floracheck™ Environmental (V3, V4, and V5 hypervariable regions)
Floracheck™ Mammalian(V3 and V4 hypervariable regions).
Floracheck Essential (V4 hypervariable region only)
Deliverables:
• Raw data as FASTQ files
• Chart detailing the type and relative abundance of bacterial and archaeal genera in each sample
Sequencing Depth:
Guaranteed 50MB data,deep coverage per request

TAT:
• 2 weeks

Sample Submission:
• Cell pellets
• High quality genomic DNA (a minimum of 100ng as quantified by Qubit 2.0)
• OD260/280 ratio is between 1.8 to 2.0
• OD260/230 ratio is between 1.5 to 2.0

Data security: biometrically restricted laboratory access,closed loop data behind firewall, and biometrically restricted data room

Promotion period:
Feburary 1, 2016 to May 24, 2016; minimum sample amount: 24

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Starting at $90.00 USD per sample
1363983501859 145375dfb8ed1b7723376b467bd3f18f

Rochester New York
28 Orders Completed
100% Positive

The listed price is includes library construction, pooling, MiSeq 2x300 run, and data analysis through OTU table generation. MiSeq runs only with or without analysis is an option.

Instrumentation: Illumina MiSeq

We can multiplex greater then 350 sample per run and we use the new v3 2x300 MiSeq reagents.

Turnaround... Show more »

The listed price is includes library construction, pooling, MiSeq 2x300 run, and data analysis through OTU table generation. MiSeq runs only with or without analysis is an option.

Instrumentation: Illumina MiSeq

We can multiplex greater then 350 sample per run and we use the new v3 2x300 MiSeq reagents.

Turnaround time: ~8-10 weeks including 16s library construction (DNA is provided) or 3-4 weeks for just the MiSeq run. Other cycle options are available depending on need.

Deliverables:

  • Raw data through OTU table data.
  • Secondary analysis can be performed using our NGS Data analysis service.

We use the protocol described here for 16s Metagenomic Analysis:

Fadrosh et al.
Microbiome. 2014 Feb 24;2(1):6. doi: 10.1186/2049-2618-2-6.

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Price on request
1362440194885 ed0a0e6a64c8dc16ec5800e3c261cc50

PrimBio Research Institute LLC

Exton Pennsylvania
18 Orders Completed
100% Positive

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive, and broad-range research analyses of mixed microbial populations. The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. The combination of the two primer pools enables broad-range, sequence-based identification of bacteria from complex mixed populations.

Price on request
1352588821707 5ab53dce542c41b945b3554179edc5d9

Deep Sequencing and Microarray Core

Baltimore Maryland
12 Orders Completed
100% Positive
Starting at $18.00 USD per sample
1381777811414 3856cab1396a93a0e15896df1e0d7aeb

Oxford Ohio
10 Orders Completed
100% Positive

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the V4 (515f/806r) region. Currently, we use the EMP primer design (old design, not the new). Contact for pricing - usually between $18 to $25 per sample based on a plate (96 samples). There is a hard floor for pricing... Show more »

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the V4 (515f/806r) region. Currently, we use the EMP primer design (old design, not the new). Contact for pricing - usually between $18 to $25 per sample based on a plate (96 samples). There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

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Starting at $42.00 USD per sample
1378568383879 082da1e423c398b034065300dda9f104

Norcross Georgia
8 Orders Completed
100% Positive

Metagenomics studies are commonly performed by analyzing the prokaryotic 16S ribosomal RNA gene (16S rRNA). The 1,500 bp gene contains nine variable regions that are used in phylogenetic classifications such as genus or species in diverse microbial populations. Either the v4 or the v3v4 region are standard offerings, however... Show more »

Metagenomics studies are commonly performed by analyzing the prokaryotic 16S ribosomal RNA gene (16S rRNA). The 1,500 bp gene contains nine variable regions that are used in phylogenetic classifications such as genus or species in diverse microbial populations. Either the v4 or the v3v4 region are standard offerings, however other regions are possible upon request.
Sequencing uses Illumina MiSeq paired end sequencing 2 x 250bp or 2 x 300bp.
Up to 380 samples can be multiplexed together into one run (plus two positive and two negative control).
The price listed assumes 184 to 190 samples pooled into 1 run. Lower multiplexing will result in a higher per sample cost. Request a quote to get your specific projects price point.
This price includes the library preparation cost starting from user provided 10ng microbial genomic DNA at 5ng/ul concentrations. We also offer microbial genomic DNA extraction as a service that can be bundled with this service as well.

Project TAT: 2-4 weeks standard, 2 weeks or less @ 15% premium (optional)
Deliverables include demultiplexed fastq files and a metagenomic analysis report for each sample.

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Price on request
1352601184186 7fffb663120dd173020ed4b9bc223583

Penn Genomic Analysis Core

Philadelphia Pennsylvania
5 Orders Completed
100% Positive

16S rRNA sequencing on Ion Torrent PGM sequencer. Scalable throughput from 500k to 5m reads, and 100Mb to 1Gb. Results in 1-3 weeks.

Price on request
1384899421685 06a9615b530ef3fed2686541df73cbdd

Laragen, Inc.

Culver City California
3 Orders Completed
100% Positive

Laragen offer 16S rRNA sequencing. This service includes
- Extract genomic DNA from bacterial colony or culture,
- PCR amplify and sequence 500 or 1200 bp of 16S rDNA with bacterial universal primers,
- Manually edit the consensus sequences from both strands.