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  • Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension.

    Curr Protoc Microbiol. Chapter 10:Unit 10A.6. doi: 10.1002/9780471729259.mc10a06s20. February 2011. View on PubMed.
  • Authors

    Treuer R and Haydel SE
  • Abstract

    Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.

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