OBJECTIVE To describe the prevalence of the CF pathogens Pseudomonas aeruginosa, Staphylococcus aureus and Haemophilus influenzae in OP cultures from healthy children. METHODS Oropharyngeal (OP) swabs were collected from 100 healthy children ≤18years of age undergoing a clinically indicated procedure. RESULTS P. aeruginosa was isolated from the OP swab of one participant, S. aureus from 48 participants (including 4 methicillin-resistant) and H. influenzae from 47 participants. Cultures from 75 participants grew one or more of these organisms (55 grew one, 19 grew 2 and one grew 3 organisms). CONCLUSION P. aeruginosa is rarely recovered from the oropharynx of healthy children ≤18years of age, while recovery of S. aureus and H. influenzae is common. It is important to understand what the "normal" prevalence of CF pathogens is in the oropharynx in order to aid interpretation of OP cultures in CF patients.