Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.
Chem Commun (Camb). 46(45):8657-8. doi: 10.1039/c0cc02657c. December 7, 2010.
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Pankaj Soni, Reetz MT, Fernández L, Gumulya Y, and Carballeira JD
Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.