Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.
Chem Commun (Camb). 46(45):8657-8. doi: 10.1039/c0cc02657c. December 7, 2010. View on PubMed.
Authors
Pankaj Soni, Reetz MT, Fernández L, Gumulya Y, and Carballeira JD
Abstract

Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.