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  • Bright ideas for chemical biology.

    ACS Chem Biol. 3(3):142-55. doi: 10.1021/cb700248m. March 20, 2008. View on PubMed.
  • Authors

    Lavis LD and Raines RT
  • Abstract

    Small-molecule fluorescent probes embody an essential facet of chemical biology. Although numerous compounds are known, the ensemble of fluorescent probes is based on a modest collection of modular "core" dyes. The elaboration of these dyes with diverse chemical moieties is enabling the precise interrogation of biochemical and biological systems. The importance of fluorescence-based technologies in chemical biology elicits a necessity to understand the major classes of small-molecule fluorophores. Here, we examine the chemical and photophysical properties of oft-used fluorophores and highlight classic and contemporary examples in which utility has been built upon these scaffolds.

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