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Ultra Performance Liquid Chromatography(UPLC) is a liquid chromatography system that utilizes sub-2 µm sized particles packed in columns as the stationary phase and a solvent mobile phase in combination with high pressure (15000 psi) instrument. Samples are introduced into the system and the components are separated based on their relative affinity for stationary and mobile phase. Then the components are detected by a suitable detector such as a mass spectrometer. UPLC gives better chromatographic resolution, performs very sensitive analysis, consumes less solvent and has higher speed as compared to conventional high performance liquid chromatography. It is utilized in several other applications such as food safety analysis, clinical research, metabolomics studies, and routine quality screens. (Credit: Muhammad Atif Khan)
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