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Supercritical Fluid Chromatography (SFC) is form of normal phase chromatography that employs a supercritical fluid such as carbon dioxide as the mobile phase. Supercritical fluids have solvating properties of a liquid and transport properties of gas that enables effective and fast separation. Typically it takes around 10 minutes to achieve separation as compared to High Performance Liquid Chromatography which takes around an hour. Since it works at low temperatures, separation of thermally labile compounds can be achieved . It is commonly employed in pharmaceutical laboratories for normal chiral analysis, chiral separation, structural analysis and purification and quantification of medicinal targets. (Credit: Muhammad Atif Khan)
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