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Ribosome display is a type of protein evolution performed entirely in vitro. Libraries of DNA variants are transcribed and translated into complexes of mRNA, ribosome, and protein. Introduction of ligands identifies the desired complexes, which are reverse transcribed back into cDNA libraries. This process can be repeated iteratively to optimize the properties of the final protein. This method may be preferred to phage, yeast, bacterial, or mRNA display since it is in vitro and independent of transformation efficiency. This process can be useful for generating peptides, proteins, and antibodies with high binding affinities. (Credit: Connie Tsai)
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