PhenoSwitch Bioscience - Expert services in mass spectrometry
Sherbrooke, Quebec, Canada
5 Quantitative Proteomics Orders38 Total Orders0 Endorsements
Chemical and Proteomic Mass Spectrometry Core Facility
at Virginia Commonwealth University
Richmond, Virginia, United States of America
9 Total Orders0 Endorsements
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Quantitative proteomics is the identification and quantification of proteins in a sample. Quantitative proteomics is usually carried out by two-dimensional gel electrophoresis (2-DE) or mass spectrophotometry (MS) or both. Quantification can be relative when the abundance of proteins are compared between samples or absolute when a known concentration of a protein is added to a sample. Unlabeled proteins can be quantitated, but often proteins are metabolically labeled, isotopically tagged, or isobarically tagged. Generally for all MS methods, proteins are removed from cells and then separated, often by affinity purification or gel electrophoresis, to collect the desired protein sample sets. These samples are then analyzed by MS. Uses for quantitative proteomics include studies of post-translational protein modifications, the identification of drug targets in drug discovery, and the identification of biomarkers by comparing diseased to healthy tissue. (Credit: Brooke Anderson-White)
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