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This top down mass spec approach to protein sequencing involves the entire protein being ionized either by electrospray ionization (ESI) or by matrix-assisted laser desorption ionization (MALDI) and then run through the mass analyzer and compared to a database of protein configurations. Mass spec can also be used to identify protein sequences de novo. (When the protein is broken down into component peptides for sequencing by enzymatic digestion, this is known as peptide mass fingerprinting.)
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