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The OneArray RNA microarray technology from Phalanx Biotech Group is a fluorescence-based assay to evaluate gene expression by messenger RNA (mRNA) level in small samples. A OneArray is created by synthesizing single-stranded 60 nucleotide DNA segments (probes) and then immobilizing them on a glass slide by thermo jet deposition. Each probe represents a single gene. Commercial OneArrays include the Human OneArray V6 and Mouse OneArray V2. To create the target sequences for a one-color assay, mRNA is purified from the sample to be analyzed, reverse transcribed, and then amplified by in vitro transcription (IVT) into single-stranded antisense RNA (aRNA). Fluorescently labeled nucleotides are incorporated during IVT and the labeled aRNA is digested. These target sequences are then hybridized to the microarray during which they bind the complementary single-stranded probes and then the unbound targets are washed away. The microarray is laser scanned by an OneArray-compatible instrument to measure the fluorescent emission. The emission is correlated to the level of transcription for each gene with OneArray-compatible software. The applications of RNA microarrays include the study of gene regulation, cell fate determination, the identification of disease-related genes, analysis of the effects of drugs on gene expression, and tumor typing for personalized cancer treatments. (Credit: Brooke Anderson-White)
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