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In Liquid Chromatography Matrix Assisted Laser Desorption/Ionization Tandem Mass Spectrometry, liquid chromatography (LC) is combined with matrix-assisted laser desorption ionization tandem mass spectrometry(MALDI-MS/MS). Sample is initially loaded in the LC system where its constituents are separated according to their relative affinity to mobile and stationary phase. An automated system transfers eluted compounds from LC onto a MALDI plate containing matrix. As a laser shines onto the plate, it ionizes and evaporates the matrix along with eluted compounds from the sample. These ions move according to mass-to-charge ratios and are detected and characterized by two mass spectrometers in series to give a mass spectrum. This can be compared to existing databases to identify the compounds in sample mixture. Ion spectra produced by such a system is utilized for protein identification in samples by searching against proteome databases. (Credit: Muhammad Atif Khan)
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