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This method involves shooting a pulsed laser at a sample adhered to a surface. The laser heats the sample rapidly enough that it vaporizes (desorbs) from the surface with very little fragmentation or decomposition. Variations on this technique, including matrix-assisted laser desorption/ionization (MALDI) and surface-enhanced laser desorption/ionization (SELDI), have proven extremely useful for the analysis of high molecular weight molecules, such as proteins.
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