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Induced Pluripotent Stem Cell (iPSC) Generation
Induced pluripotent stem cells can be generated from differentiated somatic cells by the forced expression of a few key transcription factors such as Oct4, Nanog, c-Myb and Klf4. Initially, exogenous gene expression was achieved through retroviral transduction, but this led to increased oncogenic potential of the derived iPSCs. Recently, a protein-only based method, where recombinant purified proteins of the key factors are injected into somatic cells, has been used to generate iPSCs with greatly reduced oncogenic potential.
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