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Ion exchange chromatography is used for separating molecules based on their polarity and charge. In anion exchange chromatography, negatively charged molecules are attracted to a positively charged solid support. The opposite occurs in cation exchange chromatography. The sample is introduced in a vertical column in which resin acts as the stationary phase. At low salt concentration of the mobile phase buffer, molecules in the sample reversible binding with the charged stationary phase. As salt concentration of the mobile phase is gradually increased, weakly bound components of sample begin to elute and more strongly charged molecules elute as concentration is raised further. It is commonly utilized for the separation of proteins, nucleotides and amino acids. (Credit: Muhammad Atif Khan)
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