Electron Microscopy Core Facility
at Bowling Green State University
Bowling Green, Ohio, United States of America
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Immunoelectron Microscopy
Some limited protein expression localization can be obtained by using immunogold labeling. The specific protein is localized by an antibody (similar to immunostaining methods), and the antibodies are then labeled with gold microbeads. The gold beads will show up as small electron dense spots on the resulting image. More than one protein can be localized by using beads of a different size.
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