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High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) is a technique in which the high performance liquid chromatography is used to separate the components in a sample mixture under high pressure, followed by mass analysis by two mass spectrometers in series, which ionize and characterize sample constituents by their relative abundance and mass-to-charge ratio. It requires small sample volumes and give fast analysis with high specificity. It allows for a very accurate quantification of specific components in the sample mixture and is therefore frequently employed in forensic investigations, environmental monitoring and clinical research. (Credit: Muhammad Atif Khan)
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