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Hematoxylin and eosin (H&E) is one of the principal stains in histology and a gold standard technique for diagnosis of various disease for at least a century. The staining protocol is performed on a variety of fixed/embedded cells and tissues. Hematoxylin exhibits pH indicator-like properties, being blue and less soluble in aqueous alkaline conditions, and red and more soluble in alcoholic acidic conditions. Eosin is pink and stains proteins nonspecifically. In a typical stained tissue, nuclei are stained blue, whereas the cytoplasm and extracellular matrix have varying degrees of pink staining. H&E stain discloses abundant structural information as well as specific functional implications. A limitation of H&E staining is that it is incompatible with immunofluorescence. (Credit: Noushin Nabavi)
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