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Genotyping by PCR
Genotyping by PCR is a common, cost-effective, and rapid molecular genotyping protocol that employs specific PCR primers targeting a specific DNA sequence of gene of interest in order to differentiate between varieties of genotypes. To do this, DNA is first extracted from a piece of tissue or specimen of interest. Then, a PCR reaction is run with primer targeting an area that differs between alleles of interest. The resulting reaction is loaded on an agarose gel for visualization of specific sized PCR amplicons. Included in the PCR reaction sets should be one positive control to ensure that all PCR reagents are working well and can generate a band and a negative control to ensure that none of the reagents are contaminated with unwanted DNA. The negative control contains no template and should result in no bands. (Credit: Noushin Nabavi)
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