Transgenics and Genetic Constructs Shared Resource (TGCSR)
at Geisel School of Medicine
Lebanon, New Hampshire, United States of America
1 Embryonic stem cell culture Orders2 Total Orders0 Endorsements
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Embryonic stem cell culture
Embryonic stem cells require special conditions in order to be maintained in their pluripotent state. Early culture methods relied upon plating ES cells upon a feeder layer, usually of mouse embryonic fibroblasts. Later, it was determined that these feeder cells provided a factor, LIF, that blocked differentiation of ES cells. This has led to the development of feeder-free ES culture methods that can sometimes be used instead of a feeder cell layer.
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