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Environmental scanning electron microscopy (ESEM) allows for imaging samples in their natural state, without drying or metal coating, in a gaseous chamber. As in scanning electron microscopy (SEM), ESEM involves scanning an electron beam over the surface of a sample in a raster pattern to create an image. Unlike SEM, the sample is not dried or metal coated and the sample is in a pressurized, gas-filled chamber where detectors optimized for the gaseous conditions are used to collect the electrons. The collected electrons are processed into an image of the surface of the sample. The applications of ESEM range from archeology to biology and materials science. In biology, ESEM is useful for imaging living tissues and cells or fragile collections of cells like biofilms. (Credit: Brooke Anderson-White)
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