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Chiral separation by liquid chromatography, also called chiral column chromatography, is a technique of separating enantiomers. In this technique, a single enantiomer of the chiral compound is combined with an achiral compund such as silica gel and acts as the stationary phase whereas a liquid solvent acts as the the mobile phase. The two enantiomers of the chiral compound in the sample have different affinities to the stationary phase. Thus they travel at different speeds through the column and are eluted at different times. This separation technique finds several applications in basic research and pharmaceutical industry including its use in making optically active drugs, monitoring progress of an asymmetric synthesis, and for determining optical purity of the products. (Credit: Muhammad Atif Khan)
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