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Chemical ionization refers to a method of ionizing sample molecules by a chemical reaction, rather than using direct electron bombardment. The reagent gas is first ionized, and then ionizes the sample molecules. This interaction can result in the transfer or either a proton or an electron; when a negative ion is produced, the process is termed negative ion chemical ionization. Chemical ionization is a soft ionization method, resulting in little if any fragmentation of the sample molecule, and is useful for samples with a mass less than 1,000 daltons (Da). Chemical ionization is not the same as chemi-ionization.
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