Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, high quality research tools at affordable prices. Our vision… “The Beauty of Science is to Make Things Simple” is now truer than ever. Whether it's Epigenetics, DNA, RNA, E. coli, or Yeast based research, our philosophy remains the same: to provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance.
Although historically recognized for its innovative DNA and RNA purification technologies, Zymo Research has recently received much attention for its rapidly expanding epigenetics portfolio of products. Branding ourselves “The Epigenetics Company” it is our objective to develop and provide the most comprehensive set of research tools for DNA methylation analysis and epigenetics research available today.
Thousands of peer-reviewed scientific publications from researchers around the world feature our epigenetic technologies in addition to our other products. To date, our EZ DNA Methylation™ family of products remain the most popular and cited technologies available for bisulfite treatment of DNA for methylation-specific analysis. However, we have many new technologies also developed for histone, chromatin, and small RNA analysis and for the next era of DNA methylation detection and analysis.
Reduced Representation Hydroxymethylation Profiling (RRHP) for genome-wide DNA hydroxymethylation epigenetic analysis
-Strong positive-display method avoids the need for parallel or subtractive sequencing runs
-Strand-specific, single-nucleotide detection of 5-hmC in DNA
-Small sample input (down to 100 ng)
-Simultaneous detection of SNPs
-Convenient overlay with methylation data from Zymo's Genome-Wide DNA Methylation Services
This service is for genome-wide profiling of 5-hydroxymethylcytosine (5-hmC) in DNA at single-nucleotide resolution. RRHP also allows strand-specific determination of the location of the 5-hmC modification as well as quantification of 5-hmC levels. Data from RRHP is easily integrated with DNA methylation data from Methyl-MiniSeq™, allowing for direct comparison of DNA methylation and hydroxymethylation in the same sample. Omission of chemical conversion processes allows for both low DNA input as well as straightforward SNP detection.
Available with complete and customizable bioinformatics analysis. Contact us at through Science Exchange to learn more, or to receive a personalized price quote.
Enhanced RRBS and WGBS for Genome-Wide DNA Methylation Epigenetic Analysis at Single-Base Resolution
-Next-Gen bisulfite sequencing platforms for DNA methylation analysis
-Applicable to a broad range of sample sources (human, mouse, rat, plant, etc.) and compatible with low DNA input
-Streamlined workflows with comprehensive bioinformatic analysis
-High quality publishable data delivery
-Can be customized and combined with rapid turnaround at an affordable cost
This platform is an improved version of Reduced Representation Bisulfite Sequencing (RRBS) that improves coverage and sequencing depth. It can be used to detect 3-4 million unique CpG sites, allowing >85% coverage of all CpG islands and >80% of all gene promoters for a maximal amount of methylation data from less sequencing reads, reducing the overall cost. The system is conducive to biomarker discovery by providing for the identification and analysis of differentially methylated regions (DMRs) between samples.
MidiSeq™ can be used to detect 8-9 million unique CpG sites. It extends the coverage of the Methyl-MiniSeq™ platform to include a large majority of genetic regulatory elements, gene bodies, and repeated DNA sequences. It is a good option for those researchers requiring methylome analysis outside of gene promoters and CpG islands.
The Methyl-MaxiSeq™ platform (whole-genome bisulfite sequencing, WGBS) is for the detection of DNA methylation across the entire genome. DNA methylation information is provided in CpG context as well as in the less common CHG and CHH contexts. The platform attains an average read coverage of 15-20X per base (for the human genome). This can be modified depending on your requirements. Since whole-genome sequence is provided, SNP analysis can be performed simultaneously.
Price shown is for Methyl-MiniSeq™. All platforms are available with complete and customizable bioinformatics analysis. Contact us through Science Exchange to learn more, or to receive a personalized price quote.
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