Zyagen, a biotechnology firm located in San Diego, California USA, is a leading provider of life science products and services with focus on gene expression since 2000.
Zyagen develops, manufactures, and sells quality life science products, biochemical, and reagents for cellular localization, expression, and quantitative analysis of genes and their protein products. Zyagen also offer custom research services in histology and molecular biology for pharmaceutical, biotechnology, federal, and academic research institutes worldwide.
We are continually improving and expanding our products and services line to meet the growing demand of scientists in the biomedical/life science research community for high-throughput gene expression profiling. Our goal is to simplify, improve, and accelerate research speed of the scientists and expand their research capabilities in making discoveries that improve human condition by providing the highest standard quality research products and services with competitive price.
Zyagen offers a full range of immunohistochemistry (IHC) services, including routine immunohistochemistry (peroxidase and alkaline phosphatase), immunofluorescence (IF), immunocytochemistry (ICC), apoptosis detection (TUNEL), and proliferation assays (BrdU).
IHC can be done on paraffin and frozen tissue sections of any tissue type of any species. We can use ready to stain slides provided by customers or by Zyagen. We can also process experimental tissues provided by customers for paraffin or frozen OCT embedding, sectioning, and immunostaining.
For accuracy of each IHC, negative and positive control sides will be run in parallel with the experimental tissue samples.
Our immunohistochemistry team are with 20 years of experience doing with IHC on paraffin sections, frozen sections, and cell cultured samples. Simply provide Zyagen with your samples and we do the rest.
Immunohistochemistry Services include:
- Immunohistochemistry Validation Services
- Routine Immunohistochemistry and Immunofluorescence Services
- Double Immunohistochemistry Services
- TUNEL Assay (Apoptosis Detection Assay)
- BrdU Assay (Proliferation Detection Assay)
- Sample images of Immunostaining
Zyagen has been offering a comprehensive full range of histological services for routine and research since 2000. Tissues and cells are provided by customers. Zyagen can also provide large variety of tissue types of many species for histology work requested by customers. Our customers are from pharmaceutical, biotechnology, academic, federal and preclinical research organizations. Our laboratory is a fully equipped histology facility which is staffed by well-trained professional histologists with many years of experience. Our dedication of providing high quality histology services with affordable competitive pricing and fast turn-around times make Zyagen laboratory one of the most reliable sources for histology services. No request is ever considered too small or too large.
Histology Services include:
- Tissue Fixation in any fixative of your choice at optimal fixation time
- Tissue processing, embedding, and sectioning (paraffin and/or frozen)
- Bone Decalcification and sectioning (paraffin and/or frozen). Click her for more detail
- Tissue embedding in paraffin blocks for microtome sectioning
- Tissue embedding in OCT blocks (fresh or fixed tissue) for cryostat sectioning
- Processing and embedding cell/organoid pellets in paraffin for microtome sectioning or in O.C.T. for cryostat sectioning.
- Mounting sections on positively charged slides or any other treated slides of your choice.
- Floating sections captured in wells of tissue culture plates or tubes.
- Processing intestine and colon tissues for Swiss Roll embedding and sectioning
- Processing biopsy, implant, and tumor tissues for paraffin and/or frozen sectioning.
- Computer labeling of slides
- Special handling of specimens for genomic testing and nucleic acid preparation to eliminate contamination and degradation.
- Routine Hematoxylin/Eosin (H&E) and many other special histology stains
Zyagen offers Western blot custom services including extraction of total protein from tissues or cells provided by Zyagen or customers, construction of Western blots, immunostaining blots with any antibody, quantitative analysis of immunostaining, and sendiing high quality digital images. Protein samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each protein sample whether provided by customers or Zyagen is subjected to rigorous quality control test before use. We can make blots with up to 22 tissues each plus one pre-stained protein marker.
Construction of Western blots:
Harvesting fresh tissues/cultured cells provided by Zyagen or customers.
Homogenization of tissues/cells in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors.
Quantitation of total protein using sensitive and accurate protein assay.
Testing the purity and integrity of protein by gel electrophoresis.
Fractionation of protein (75 microgram) through large SDS-denatured polyacrylamide gel electrophoresis.
Transfer protein onto PVDF membranes.
Shipping blots at room temperature in sealed bags
Western Blot Analysis:
Extraction of total protein from tissues/cells.
Constructions of Western blots using total protein provided by Zyagen or customers
Immunostaining blots with antibodies provided by customers using ultrasensative chemiluminescent ECL detection
Quantitative analysis of immunostaining
Sending high quality digital images of X-ray films
Zyagen offers cell-based assay including apoptosis, cell cycle analysis, cell differentiation, cell proliferation, and cytotoxicity to identify and quantify cell death and viability in different cell types and tissues provided by clients.
Zyagen offers ELISA services to quantify and analyze protein of your interest in any biological samples by direct and sandwich ELISA. Service include protein extraction, antobody characterization, immunoassay, and quantitative analysis.
(Modified Masson's): For visualization of collagenous connective tissue fibers in tissue sections. Collagen muscle fibers stai blue and muscle fibers red.
For visualization of nucleic acids and highlightcomponents in tissues such as mast cells granules, mucins, and cartilage.Nucleic acids stain blue, mast cells stain red purple (metachromatic staining) with blue background.
For visualization of polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissue sections. Positive PAS stain magenta. Nuclei stain black/blue.
(Modified Brown & Brenn): For visualization of gram positive bacteria.
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