1376675112296 de71dbbc203fb50680135447b8f1c385

Zyagen

San Diego, California, US

Zyagen, a biotechnology firm located in San Diego, California USA, is a leading provider of life science products and services with focus on gene expression since 2000.

Zyagen develops, manufactures, and sells quality life science products, biochemical, and reagents for cellular localization, expression, and quantitative analysis of genes and their protein products. Zyagen also offer custom research services in histology and molecular biology for pharmaceutical, biotechnology, federal, and academic research institutes worldwide.

We are continually improving and expanding our products and services line to meet the growing demand of scientists in the biomedical/life science research community for high-throughput gene expression profiling. Our goal is to simplify, improve, and accelerate research speed of the scientists and expand their research capabilities in making discoveries that improve human condition by... Show more »

Zyagen, a biotechnology firm located in San Diego, California USA, is a leading provider of life science products and services with focus on gene expression since 2000.

Zyagen develops, manufactures, and sells quality life science products, biochemical, and reagents for cellular localization, expression, and quantitative analysis of genes and their protein products. Zyagen also offer custom research services in histology and molecular biology for pharmaceutical, biotechnology, federal, and academic research institutes worldwide.

We are continually improving and expanding our products and services line to meet the growing demand of scientists in the biomedical/life science research community for high-throughput gene expression profiling. Our goal is to simplify, improve, and accelerate research speed of the scientists and expand their research capabilities in making discoveries that improve human condition by providing the highest standard quality research products and services with competitive price.

« Show less

Zyagen has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Northern Blot
Price on request

Zyagen offers Northern blot analysis services to extract total RNA, microRNA, purification of mRNA, isolation of genomic DNA and construct ready-to-hybridize Northern blots. RNA/mRNA, DNA samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each sample whether provided by the customers... Show more »

Zyagen offers Northern blot analysis services to extract total RNA, microRNA, purification of mRNA, isolation of genomic DNA and construct ready-to-hybridize Northern blots. RNA/mRNA, DNA samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each sample whether provided by the customers or Zyagen is subjected to rigorous quality control test before use. We can make blots with up to 20 tissues each plus one RNA or DNA marker.

Construction of Northern Blot Services:
- Isolation total RNA or mRNA from tissues provided by Zyagen or customers.
- Fractionation of RNA (20 microgram of total RNA or 2 microgram of poly A+ RNA per lane) through formaldehyde-denatured agarose gel.
- Transfer RNA onto nylon supercharged membranes.
- Cross-linked RNA by UV light.
- Staining blots with reversible staining (Ponceau) to check the sharpness of RNA bands and efficiency of transfer.
- Sealed blots in bags and shipped at room temperature.

« Show less
Western Blot
Price on request

Zyagen offers Western blot custom services including extraction of total protein from tissues or cells provided by Zyagen or customers, construction of Western blots, immunostaining blots with any antibody, quantitative analysis of immunostaining, and sendiing high quality digital images. Protein samples are extracted from tissues... Show more »

Zyagen offers Western blot custom services including extraction of total protein from tissues or cells provided by Zyagen or customers, construction of Western blots, immunostaining blots with any antibody, quantitative analysis of immunostaining, and sendiing high quality digital images. Protein samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each protein sample whether provided by customers or Zyagen is subjected to rigorous quality control test before use. We can make blots with up to 22 tissues each plus one pre-stained protein marker.


Construction of Western blots:

Harvesting fresh tissues/cultured cells provided by Zyagen or customers.
Homogenization of tissues/cells in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors.
Quantitation of total protein using sensitive and accurate protein assay.
Testing the purity and integrity of protein by gel electrophoresis.
Fractionation of protein (75 microgram) through large SDS-denatured polyacrylamide gel electrophoresis.
Transfer protein onto PVDF membranes.
Shipping blots at room temperature in sealed bags

Western Blot Analysis:

Extraction of total protein from tissues/cells.
Constructions of Western blots using total protein provided by Zyagen or customers
Immunostaining blots with antibodies provided by customers using ultrasensative chemiluminescent ECL detection
Quantitative analysis of immunostaining
Sending high quality digital images of X-ray films

« Show less
Apoptosis Assays
Price on request

Zyagen offers cell-based assay including apoptosis, cell cycle analysis, cell differentiation, cell proliferation, and cytotoxicity to identify and quantify cell death and viability in different cell types and tissues provided by clients.

Zyagen offers cell-based assay including apoptosis, cell cycle analysis, cell differentiation, cell proliferation, and cytotoxicity to identify and quantify cell death and viability in different cell types and tissues provided by clients.

« Show less
ELISA
Enzyme-linked immunosorbent assay
Price on request

Zyagen offers ELISA services to quantify and analyze protein of your interest in any biological samples by direct and sandwich ELISA. Service include protein extraction, antobody characterization, immunoassay, and quantitative analysis.

Zyagen offers ELISA services to quantify and analyze protein of your interest in any biological samples by direct and sandwich ELISA. Service include protein extraction, antobody characterization, immunoassay, and quantitative analysis.

« Show less
Southern Blot
Price on request

Zyagen offers Southern blot analysis services to extract total RNA, microRNA, purification of mRNA, isolation of genomic DNA and construct ready-to-hybridize southern blots. RNA/mRNA, DNA samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each sample whether provided by the customers... Show more »

Zyagen offers Southern blot analysis services to extract total RNA, microRNA, purification of mRNA, isolation of genomic DNA and construct ready-to-hybridize southern blots. RNA/mRNA, DNA samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each sample whether provided by the customers or Zyagen is subjected to rigorous quality control test before use. We can make blots with up to 20 tissues each plus one RNA or DNA marker.

Construction of Southern Blots Services:

  • Isolation DNA from tissues provided by Zyagen or customers.
  • Fractionation of DNA (20 microgram of DNAper lane) through denatured agarose gels.
  • Transfer DNA onto supercharged membranes.
  • Cross-linked DNA by UV light.
  • Staining blots with reversible staining (Ponceau) to check the sharpness of DNA bands and efficiency of transfer.
  • Sealed blots in bags and shipped at room temperature.
  • Northern/Southern blotting custom services ordering information:
« Show less
Tissue Sectioning
Price on request
  • Brain Tissue Sectioning
  • Embryo Tissue sectioning
  • Cell Processing and sectioning
  • Bone Decalcification and sectioning
  • DNA and RNA Extraction from FFPE
  • Brain Tissue Sectioning
  • Embryo Tissue sectioning
  • Cell Processing and sectioning
  • Bone Decalcification and sectioning
  • DNA and RNA Extraction from FFPE
« Show less
Masson's Trichrome Staining
Price on request

(Modified Masson's): For visualization of collagenous connective tissue fibers in tissue sections. Collagen muscle fibers stai blue and muscle fibers red.

(Modified Masson's): For visualization of collagenous connective tissue fibers in tissue sections. Collagen muscle fibers stai blue and muscle fibers red.

« Show less
Toluidine Blue Staining
Price on request

For visualization of nucleic acids and highlightcomponents in tissues such as mast cells granules, mucins, and cartilage.Nucleic acids stain blue, mast cells stain red purple (metachromatic staining) with blue background.

For visualization of nucleic acids and highlightcomponents in tissues such as mast cells granules, mucins, and cartilage.Nucleic acids stain blue, mast cells stain red purple (metachromatic staining) with blue background.

« Show less
Periodic Acid-Schiff (PAS) Staining
Price on request

For visualization of polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissue sections. Positive PAS stain magenta. Nuclei stain black/blue.

For visualization of polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissue sections. Positive PAS stain magenta. Nuclei stain black/blue.

« Show less
Gram Staining
Price on request

(Modified Brown & Brenn): For visualization of gram positive bacteria.

(Modified Brown & Brenn): For visualization of gram positive bacteria.

« Show less
Biospecimens
Price on request
Request a quote for more information about this service.
RNA
Price on request
Request a quote for more information about this service.
Protein Quantification
Price on request
Request a quote for more information about this service.
Common Histochemical Stains
Price on request
Request a quote for more information about this service.
Clinical Research
Price on request
Request a quote for more information about this service.
Cell-Based Assays
Price on request
Request a quote for more information about this service.
Clinical Laboratory Services
Price on request
Request a quote for more information about this service.
Histological Staining
Price on request
Request a quote for more information about this service.
Tissue Processing
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
DNA Fragment Analysis
Price on request
Request a quote for more information about this service.
Cell Death Assays
Price on request
Request a quote for more information about this service.
Protein Services
Price on request
Request a quote for more information about this service.
Cells and Tissues
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
Protein Expression Visualization
Price on request
Request a quote for more information about this service.
DNA
Price on request
Request a quote for more information about this service.
Clinical and Anatomic Pathology
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.

Zyagen has not received any ratings.

Be the first to endorse Zyagen

Endorse this lab