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zPREDICTA

San Jose, California, US

zPREDICTA offers physiologically relevant cell culture models that recapitulate the major stages of human cancers. Utilizing the 3D tissue culture systems and spheroid assays, we help our clients study tumor formation, invasion, and metastasis – from studies of basic biology to target discovery and complex preclinical screens of investigational compounds and drug combinations. Our 3D cultures are customized to the tissue and cancer of interest and provide researchers access to enriched cell populations representing the primary tumor, as well as invasive, metastatic, and cancer stem cells.

Our models incorporate tissue microenvironment of the primary tumor and the distant metastatic site recapitulating the elements of environment-mediated drug-resistance in the context of:

• tissue-specific extracellular matrix (ECM)
• stromal-derived soluble factors
• tumor-tumor interactions
• tumor-stroma... Show more »

zPREDICTA offers physiologically relevant cell culture models that recapitulate the major stages of human cancers. Utilizing the 3D tissue culture systems and spheroid assays, we help our clients study tumor formation, invasion, and metastasis – from studies of basic biology to target discovery and complex preclinical screens of investigational compounds and drug combinations. Our 3D cultures are customized to the tissue and cancer of interest and provide researchers access to enriched cell populations representing the primary tumor, as well as invasive, metastatic, and cancer stem cells.

Our models incorporate tissue microenvironment of the primary tumor and the distant metastatic site recapitulating the elements of environment-mediated drug-resistance in the context of:

• tissue-specific extracellular matrix (ECM)
• stromal-derived soluble factors
• tumor-tumor interactions
• tumor-stroma interactions

Our culture systems have been validated both in vitro and in vivo and were tested on multiple cancer cell lines and primary tissues representing multiple types of solid tumors and hematologic malignancies. Our models mimic in vivo behavior of tumors and exhibit high correlation with clinical response.

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3D Cell Culture Models
Price on request

In 3D cell culture systems cells grow under physiological conditions in the context of tissue-specific microenvironment. Cells are grown in extracellular matrix (ECM) (i.e. Matrigel, collagens, fibronectin, hyaluronic acid, etc.) or a combination of matrices formulated to reconstruct the tissue of interest.

Any assays... Show more »

In 3D cell culture systems cells grow under physiological conditions in the context of tissue-specific microenvironment. Cells are grown in extracellular matrix (ECM) (i.e. Matrigel, collagens, fibronectin, hyaluronic acid, etc.) or a combination of matrices formulated to reconstruct the tissue of interest.

Any assays performed in standard cell culture can be performed in 3D culture, including, but not limited to:

• Morphology analysis
• Cell proliferation (MTS assay, Ki67 staining, etc.)
• Cell migration (migration through ECM, chemoattraction, etc.)
• Cell viability (MTS assay, annexin V staining, Live/Dead analysis, etc.)
• Preclinical analysis (response to treatment, dose response, timecourse, LC50/LC10)
• Drug interactions (synergistic, additive, antagonistic)
• Target and biomarker discovery (cell fractionation to isolate enriched populations of cells representing various subsets of heterogeneous tumors)
• Immunofluorescence staining for proteins of interest
• Custom assays

ANALYSIS:
• Microscopy (brightfield, immunofluorescence)
• Flow cytometry
• Plate reader
• Multitude of analysis capabilities is available, please inquire about specific instrumentation

TURNAROUND TIME: 7-30 days (depending on culture type and desired analysis)

ASSAY FORMAT: multiwell plates (96, 48, 24, 12, 6-well). The format is dependent on the cell number requirements as determined by the investigator.

DELIVERABLES:
• Data (raw, analyzed)
• Images (raw, analyzed)
• Cell lysates for RNA, DNA, or protein isolation
• Fixed cells (pellets or slides)
• Frozen blocks of OTC embedded cells
• Please inquire about additional deliverables

MATERIALS:
Cell lines: from our library of cells, provided by the investigator, purchased for the project
Primary cells: provided by the investigator
Drugs: provided by the investigator

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Cancer
Drug Potency and Efficacy Testing
Price on request

Our 3D cell culture platform is designed to evaluate investigational anticancer therapeutics and drug combinations within the native microenvironment of the human tissues. Our pipeline allows testing new agents under the physiological conditions of environment-mediated drug resistance (EM-DR). We have the capacity to evaluate... Show more »

Our 3D cell culture platform is designed to evaluate investigational anticancer therapeutics and drug combinations within the native microenvironment of the human tissues. Our pipeline allows testing new agents under the physiological conditions of environment-mediated drug resistance (EM-DR). We have the capacity to evaluate novel therapeutics on cells of the primary tumor, as well as invasive, metastatic, and cancer stem cell populations.

Utilizing the 3D cell culture platform we can evaluate a number of parameters in the context of environment-mediated drug resistance (EM-DR):
• Identify patient population(s) responsive to the drug of interest
• Screen lead compounds/drug candidates to: 1) establish under various conditions of EM-DR and 2) ascertain the source(s) of resistance, i.e. identify the components of tissue microenvironment that may impede drug function
• Identify drug combinations that can overcome EM-DR
• Evaluate off-target effects and gauge potential toxicity (test cytotoxic effects of investigational compounds on non-malignant cell populations (epithelial, stromal, and hematopoietic))
• Evaluate the capacity of the investigational compounds to target invasive, metastatic and cancer stem cell populations
• Functional fractionation of heterogeneous tumors for target and biomarker discovery
• Determination of growth capacity of various cell populations

ASSAYS:
• Morphology analysis
• Cell proliferation (MTS assay, Ki67 staining, etc.)
• Cell migration (migration through ECM, chemoattraction, etc.)
• Cell viability (MTS assay, annexin V staining, Live/Dead analysis, etc.)
• Preclinical analysis (response to treatment, dose response, timecourse, LC50/LC10)
• Drug interactions (synergistic, additive, antagonistic)
• Target and biomarker discovery (cell fractionation to isolate enriched populations of cells representing various subsets of heterogeneous tumors)
• Custom assays

DELIVERABLES:
• Data (raw, analyzed)
• Images (raw, analyzed)
• Cell lysates for RNA, DNA, or protein isolation
• Fixed cells (pellets or slides)
• Frozen blocks of OTC embedded cells
• Please inquire about additional deliverables

MATERIAL:
Cell lines: from our library of cells, provided by the investigator, purchased for the project
Primary cells: provided by the investigator
Drugs: provided by the investigator

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Stem Cell Assays
Price on request

ASSAYS:
• CFU (colony forming unit) assay for hematopoietic stem and progenitor cells
• CFU-F (colony forming unit fibroblast assay)
• Spheroid assays for cancer stem cells (mammospheres, prostate spheres, neurospheres, etc.)
• Response to therapeutics (dose response, timecourse, LC50/LC10)
• Drug interactions (synergistic,... Show more »

ASSAYS:
• CFU (colony forming unit) assay for hematopoietic stem and progenitor cells
• CFU-F (colony forming unit fibroblast assay)
• Spheroid assays for cancer stem cells (mammospheres, prostate spheres, neurospheres, etc.)
• Response to therapeutics (dose response, timecourse, LC50/LC10)
• Drug interactions (synergistic, additive, antagonistic)
• Custom assays

ANALYSIS:
• Microscopy (brightfield, immunofluorescence)
• Flow cytometry
• Multitude of analysis capabilities is available, please inquire about specific instrumentation

DELIVERABLES:
• Data (raw, analyzed)
• Images (raw, analyzed)
• Cell lysates for RNA, DNA, or protein isolation
• Fixed cells (pellets or slides)
• Frozen blocks of OTC embedded cells
• Please inquire about additional deliverables

MATERIAL:
Cell lines: from our library of cells, provided by the investigator, purchased for the project
Primary cells: provided by the investigator
Drugs: provided by the investigator

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Biology
Price on request
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Cell-Based Assays
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Functional & Cell Type Specific Assays
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Cells and Tissues
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Mammalian Cell Culture
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Pharmacology & Toxicology
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Cell and Tissue Culture
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Cell and Tissue Culture Services

Cell and Tissue Culture Services

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2018-01-19 04:28:49 +0600

Net Promoter Score of 10 received for 3D Cell Culture Models.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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