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Yurogen Biosystems LLC

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Worcester, Massachusetts, US

About Yurogen Biosystems LLC

Yurogen Biosystems is an innovative biotech company specialized in monoclonal antibody development and production. We are located in the world-class biotech and biopharma center, the Greater Boston area.

At Yurogen, we engage in the full scope of monoclonal antibody development from antigen design and... Show more »

Yurogen Biosystems is an innovative biotech company specialized in monoclonal antibody development and production. We are located in the world-class biotech and biopharma center, the Greater Boston area.

At Yurogen, we engage in the full scope of monoclonal antibody development from antigen design and production, antibody screening, to antibody engineering, antibody production. Yurogen has established pipelines of monoclonal antibodies from both rabbits and mice that are widely used in in vitro diagnostics (IVD) in various formats including ELISA, TIA, and IHC. Our rigorously validated monoclonal antibodies are reliable and cost-efficient materials that meet your diverse diagnostic needs.

Yurogen has developed a next-generation single cell based SMabTM platform that can efficiently screen monoclonal antibodies from a variety of host animals. The SMabTM platform significantly shortens the monoclonal antibody screening process while yielding high affinity monoclonal antibodies with broad diversity. It is a robust platform open for CRO services that can fulfil your specific antibody requirements.

Currently, we primarily customize rabbit monoclonal antibodies to meet specific needs of our clients. Powered by SMabTM technology, Yurogen delivers well characterized recombinant rabbit monoclonal antibodies with consistent performance for a wide range of applications including sandwich ELISA, western blotting, immunofluorescent staining and IHC.
Our rabbit monoclonal antibody service is a royalty-free and cost-effective solution for high quality rabbit monoclonal antibodies.

In addition, our versatile SMabTM technology allows us to produce high quality antibodies from a variety of hosts, meeting our clients’ various antibody needs in an efficient and cost-effective manner—all part of our goal to be the one-stop solution for your antibody needs.

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Small business

Our Services (15)


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Nanobody Production

Single Domain Antibody Production
Price on request

Camel Nanobody Development Service

  1. A dedicated farm with no less than 20 healthy camels of immunization age.
  2. A well-established platform for phage display, nanobody identification and expression, and specificity assay.
  3. Self-developed key technology & experienced scientists for nanobody expression and modification.
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Camel Nanobody Development Service

  1. A dedicated farm with no less than 20 healthy camels of immunization age.
  2. A well-established platform for phage display, nanobody identification and expression, and specificity assay.
  3. Self-developed key technology & experienced scientists for nanobody expression and modification.
  4. Nanobody High Throughput Screening (NHTS) system and Bioinformatic Analysis System.
  5. Extensive expertise in therapeutic nanobody development.

Material Requirement:

1.10 mg of peptide (purity>95%, length >12 amino acids, carrier protein should be conjugated for increasing immunogenicity of peptide) or 5 mg of soluble protein with or without tags (concentration>0.5 mg/mL, purity>90%, molecular weight>20 kDa) for immunization.
- Additional 5 mg peptide or soluble protein (without carrier protein or tags) for subsequent experiments.
- Details of the peptide or protein should be addressed in the accessory.

Note: If the tag of immunized soluble protein cannot be eliminated, it should be replaced by other tags in order to avoid the enrichment of the tag-specific phage particles.

Deliverables:

Choose up to five clones. Deliverables for each clone include:

  1. Full length of selected nanobody genes in non-proprietary plasmid;
  2. The gene sequences of selected nanobodies.
  3. About 100 μg purified nanobodies for each candidate.
  4. Final report.
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Camelids

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Antibody Humanization

Price on request
  1. An in-house antibody database with 30,000+ sequences (germline, mature, recombinant) and 600+ crystal structures.
  2. Uses a proprietary homology-scoring algorithm on the basis of sequence & structure similarity to select the top-score human antibody frameworks.
  3. Uses a proprietary 3-D structure modeling-based approach to... Show more »
  1. An in-house antibody database with 30,000+ sequences (germline, mature, recombinant) and 600+ crystal structures.
  2. Uses a proprietary homology-scoring algorithm on the basis of sequence & structure similarity to select the top-score human antibody frameworks.
  3. Uses a proprietary 3-D structure modeling-based approach to identify the human FR, Vernier zone, and canonical structure residues to guide selecting potential residues that need to be back mutated to restore CDR conformation and optimal antigen binding.
  4. T-Cell epitope screening is included in the humanization design stage to remove possibly immunogenic sequences.
  5. Flexible packages are available to meet customer requirements. You may choose to include/exclude however many stages in your final package. (eg. SDR grafting is included in premium package to further improve the humanness).
  6. Antibody humanization from any parental species (eg. mouse, rat, rabbit, camel). For mouse and rabbit species, we guarantee humanized antibodies retain >80% binding affinity of the parental antibody.

Material Requirement:

  1. Hybridoma cells (mouse, rabbit, et al.) or Sequences of VL and VH.
  2. Antigen and assay protocol for measuring binding affinity (preferably ELISA).

Deliverables:

  1. VL and VH sequences of chimeric antibody and 3 humanized antibodies in pUC19 vector.
  2. Purified chimeric antibody and 3 humanized antibodies (1mg each in human IgG1 format).
  3. Study report including experiment protocols, purified antibody QC, binding affinity data.
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Antibody Affinity Maturation

Price on request
  1. Uses a proprietary computational algorithm to predict hot-spot CDR residues and introduce mutations using ambiguous codons.
  2. A novel small perturbation mutagenesis strategy for effective and efficient diversification of multiple CDRs.
  3. Construction of scFv or Fab sub-libraries using massive microchip-synthesized... Show more »
  1. Uses a proprietary computational algorithm to predict hot-spot CDR residues and introduce mutations using ambiguous codons.
  2. A novel small perturbation mutagenesis strategy for effective and efficient diversification of multiple CDRs.
  3. Construction of scFv or Fab sub-libraries using massive microchip-synthesized oligonucleotides with large diversity and low redundancy.
  4. Highly adaptive procedures to optimize experimental conditions for recombinant phage production, affinity-driving panning, binding ELISA, et al.
  5. Flexible packages are available to meet customer requirements. You may choose to include/exclude however many stages in your final package (eg. Sanger or next-generation sequencing; single or combinational CDR library screening).
  6. Significant affinity improvement can be achieved for most antibodies. We guarantee >4-fold affinity increase for single CDR library screening and >10-fold affinity increase for combinational CDR library screening.

Material Requirement:

  1. Target antibody sequences of VL and VH.
  2. Antigen and assay protocol for measuring binding affinity (preferably ELISA).

Deliverables:

  1. VL and VH sequences of 3 top-affinity variants in pUC19 vector.
  2. Purified target antibody and 3 top-affinity variants (1mg each in human IgG1 format)
  3. Study report including experiment protocols, purified antibody QC, binding affinity data.
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Monoclonal Antibody (mAb) Production

Price on request

Advantages of Rabbit Monoclonal Antibodies (RmAbs)

  1. High affinity: Typically, RmAbs have 10 – 100 times higher affinity than mouse monoclonal antibodies. On average, the KD values of RmAbs range from 10^-10 up to 10^-12 M
  2. Superior specificity: The development of primary and secondary antibody repertoires in rabbits... Show more »

Advantages of Rabbit Monoclonal Antibodies (RmAbs)

  1. High affinity: Typically, RmAbs have 10 – 100 times higher affinity than mouse monoclonal antibodies. On average, the KD values of RmAbs range from 10^-10 up to 10^-12 M
  2. Superior specificity: The development of primary and secondary antibody repertoires in rabbits possesses unique characteristics, such as gut-associated lymphoid tissues (GALT) B cell development and multiple selection procedures. These unique characteristics may account for the improved quality of the antibodies produced in rabbits [4,5]. The high specificity of RmAbs has clear edges in assays:
    • Improved assay sensitivity
    • Increased assay accuracy with decreased false positive rate
    • Lower assay noise on paraffin-embedded tissue samples--- ideal choice for IHC[6]
  3. Broad epitope recognition: Due to the reduced immunodominance, rabbit antigen presenting cells can present a broader range of epitopes than their mouse counterparts. Many less abundant proteins or epitopes can be well presented and recognized by rabbits. Rabbits are therefore able to generate high affinity antibodies against difficult epitopes, such as protein modifications, enzyme cleavage sites, or conformational epitopes.
    Partially due to the expression of all three subclasses of CD1 proteins, the nonpeptidic epitope presenting in rabbits is more efficient and can handle broader targets than that in mice. As a result, rabbits are widely adopted as preferable hosts to generate better antibodies to small molecules and haptens, including small molecule drugs, steroid hormones, lipids, and glycolipids.
  4. Great Stability: Rabbit IgG has extra disulfide bonds in the variable region of the heavy chain; an extra disulfide bond also exists between Vκ and Cκ. These extra disulfide bonds may result in the great stability and long shelf life of rabbit antibodies.

References

  • Schiaffella E, Sehgal D, Anderson A, Mage R. J Immunol. 1999;162:3984-95.
  • Pan R, Chen Y, Vaine M, Hu G, Wang S, Lu S, Kong XP. Emerg Microbes Infect. 2015 Jul;4(7)
  • Gendusa R, Scalia C, Buscone S, Cattoretti G. J Histochem Cytochem. 2014;62:519-531
  • Rossi S, Laurino L, Furlanetto A, Chinellato S, Orvieto E, Canal F, et al. Am J Clin Pathol. 2005;124:295-302
  • Rocha R, Nunes C, Rocha G, Oliveira F, Sanches F, Gobbi H. Pathol Res Pract. 2008;204:655-62
  • Vilches-Moure J, Ramos-Vara J. J Vet Diagn Invest. 2005;17:346-50
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Rabbit

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Protein Services

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Antibody Services

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Biology

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Antibody Purification

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Polyclonal Antibody Production

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Antibody Modification

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Therapeutic Monoclonal Antibody (mAb) Development

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Bioconjugation/Chemical Crosslinking

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Phage Display Library Construction and Screening

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Cell-Based Assays

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Biochemistry & Molecular Biology

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Hai Wu

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