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Yaazh Xenomics

1 Order Completed
Madurai, Tamil Nadu, IN

About Yaazh Xenomics

Yaazh Xenomics is one of the leading Genomics service and analytical service Provider in India. Our scienti c approach and Latest technology portfolio has given Excellent Results. With over a decade of market experience, supported by a team of qualified Technical Experts. We provide innovative and... Show more »

Yaazh Xenomics is one of the leading Genomics service and analytical service Provider in India. Our scienti c approach and Latest technology portfolio has given Excellent Results. With over a decade of market experience, supported by a team of qualified Technical Experts. We provide innovative and highly efficient solutions. Our team's primary objective is to identify and offer the best solution at economical cost & minimal turn round time.

Some of our Clients' Publications

- Isolation and characterization of Exopolysaccharide producing bacteria from Pak Bay (Mandapam) Parthiban Karuppiah, Vignesh Venkatasamy and Thirumurugan Ramasamy. International Journal of Oceanography & Marine Ecological System. 3(1):1-8,2014.

- Samuel, Melvin S., and Ramalingam Chidambaram. "Hexavalent chromium biosorption studies using Penicillium griseofulvum MSR1 a novel isolate from tannery effluent site: Box–Behnken optimization, equilibrium, kinetics and thermodynamic studies." Journal of the Taiwan Institute of Chemical Engineers (2014).

- Singh, Mrinalini J., and S. Padmavathy. "Isolation, screening and characterization of uranium microremediable actinomycetes from fallen leaves of Azadirachta indica in Western Ghats." Journal of Radioanalytical and Nuclear Chemistry 302.3 (2014): 1303-1307.

- Patel, P. V., and P. B. Desai. "Study of Clostridium difficile in South Gujarat region of India." Research Journal of Recent Sciences ISSN 2277: 2502.

- Mathivanan, K., and R. Rajaram. "Isolation and characterisation of cadmium-resistant bacteria from an industrially polluted coastal ecosystem on the southeast coast of India." Chemistry and Ecology 30.7 (2014): 622-635.

- Samuel Melvin, S. "Biosorption of Cr (VI) by Ceratocystis paradoxa MSR2 Using Isotherm Modelling, Kinetic Study and Optimization of Batch Parameters Using Response Surface Methodology." PloS one 10.3 (2015).

- Melvin Samuel, S. "Isotherm Modelling, Kinetic Study and Optimization of Batch Parameters Using Response Surface Methodology for Effective Removal of Cr (VI) Using Fungal Biomass." PloS one 10.3 (2015).

- Kailasan, Nair Sanjeev, and Vakil Babu Vamanrao. "Isolation and Characterization of Ralstonia Pickettii-A Novel Phosphate Solubilizing Bacterium from Pomegranate Rhizosphere from Western India." (2015).

- Singh, Mrinalini J., and Padmavathy Sedhuraman. "Biosurfactant, polythene, plastic, and diesel biodegradation activity of endophytic Nocardiopsis sp. mrinalini9 isolated from Hibiscus rosasinensis leaves." Bioresources and Bioprocessing 2.1 (2015): 1-7.

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Our Services (49)


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Sanger Sequencing

Starting at $3.50 per reaction

DNA Sequencing technique is a process to determine the order of nucleotide sequence of the particular DNA fragment. Most of the DNA sequencing method performed by using the chain termination method, which was developed by the scientist Frederick Sanger.
This Sanger sequencing technique used to sequence Plasmid or PCR product with... Show more »

DNA Sequencing technique is a process to determine the order of nucleotide sequence of the particular DNA fragment. Most of the DNA sequencing method performed by using the chain termination method, which was developed by the scientist Frederick Sanger.
This Sanger sequencing technique used to sequence Plasmid or PCR product with specific primer.
Optimized protocol for short and long read length
Quality - ~1000 to 1200bp of readable bases with Phred score higher than 20 in more than 700bp guaranteed.
Special features

Result Delivery:
Sequence With Chromatogram (*.ab1 & .pdf file format)
Sequence in text format (
.txt )

Sample Requirement:
(Check your samples concentration before sending samples to us)
To get good quality sequence data - concentration per ul is very important.
• Plasmid : Concentration 100 -150 ng/ul, Volume 20ul
• PCR product (purified) : Concentration 50ng/ul, Volume 20ul
• PCR product (non-purified) : Concentration 100ng/ul, Volume 20~30
Primer Requirements:


• Concentration : 10pmole/ul, Volume : 20ul For 5 Reactions: 20ul, as the reaction number increases increment 2-3ul each.

• Suitable Primer Tm: ~55-60°C

Always send extra volume (sample/primer) it may be used for rerun when quality of sequence read needs improvement.


• YaazhXenomics will provide Universal primers @ free of cost
Sample Requirements: (Check your samples concentration before sending samples to us)
To get good quality sequence data - concentration per ul is very important.
• Plasmid : Concentration 100 -150 ng/ul, Volume 20ul
• PCR product (purified) : Concentration 50ng/ul, Volume 20ul
• PCR product (non-purified) : Concentration 100ng/ul, Volume 20~30
Primer Requirements:


• Concentration : 10pmole/ul, Volume : 20ul For 5 Reactions: 20ul, as the reaction number increases increment 2-3ul each.

• Suitable Primer Tm: ~55-60°C

Always send extra volume (sample/primer) it may be used for rerun when quality of sequence read needs improvement.


• YaazhXenomics will provide Universal primers @ free of cost

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PCR Product Purification

Starting at $1.00 per sample
Request a quote for more information about this service.

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Oligonucleotide Synthesis

Starting at $0.10 per base

10nmol Oligo sysnthesis. Other concentrations also available.

10nmol Oligo sysnthesis. Other concentrations also available.

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16S rRNA Sequencing

Starting at $28.00 per sample

Identification based on the 16s rRNA gene sequencing has become the important tool for Researchers. We Yaazh Xenomics will do 16s rRNA sequencing for Bacteria and Actinomycetes identification; In all cells, rRNA Gene is most conserved region. For Bacteria and Actinomycetes 16S rRNA gene is amplified by using the universal primers... Show more »

Identification based on the 16s rRNA gene sequencing has become the important tool for Researchers. We Yaazh Xenomics will do 16s rRNA sequencing for Bacteria and Actinomycetes identification; In all cells, rRNA Gene is most conserved region. For Bacteria and Actinomycetes 16S rRNA gene is amplified by using the universal primers 27F/1427R and sequencing with 518F/800R. To get ~1400 bps sequences, the forward and reverse sequence properly assembled, and contigs prepared.

Our 16s rRNA sequencing services provides complete solution (DNA extraction, PCR amplification, Gel verification, Purification, Sequencing, Sequence assembling, Bioinformatics analysis (BLAST in Various Databases) and Construction of Phylogenetic tree. We also accept DNA samples, PCR products (Purified/Unpurified) for Sequencing. According to the samples charges may vary. For better result submit direct microbial culture plate or slant.

We provide below contents documents as a 16s rRNA Result:

  1. Sequence raw data (Forward and Reverse) in different file format.
  • .ab1 - Sequence with chromatogram
  • .pdf - Sequence with Chromatogram
  • .txt - Only sequence
  • .fasta - Only Sequence
  • Assembled Sequence (Contig) - ~ 1400bp or more
  • Blast result generated from All GenBank + NCBI+EMBL+DDBJ+PDB sequences.
  • Summary report comprises:
    • Methodology for DNA Extraction, PCR, Sequencing and Bioinformatic analysis.
    • Sequence analysis report contains sequence read Normal Length; Q16 & Q20 read length, GC Content details.
    • Proper contigs sequence with coverage image
    • Final Result
    • Phylogeny Tree.

We Provide all the bioinformatics analysis including Phylogenetic tree & Customer specific gene analysis with free of cost.

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18S rRNA Sequencing

Starting at $28.00 per sample

Currently, 18s rRNA Sequencing study is getting more popular to define Fungi and Algae species by Using Sequence information. We Yaazh Xenomics, usually sequences the 18s rRNA ITS region to identify the fungi and algae. We usually use the ITS1/ITS4 primer for amplifying and sequencing of ITS region. If not amplifying in the above... Show more »

Currently, 18s rRNA Sequencing study is getting more popular to define Fungi and Algae species by Using Sequence information. We Yaazh Xenomics, usually sequences the 18s rRNA ITS region to identify the fungi and algae. We usually use the ITS1/ITS4 primer for amplifying and sequencing of ITS region. If not amplifying in the above said primers we use ITS2, ITS3 and ITS5 primer combinations. With this ITS sequence data we can find out the similarity of given microorganism with various gene bank NCBI, EMBL, DDBJ, PDB sequence database and calculate the statistical similarity of matches between rRNA databases and target fungus or algae.

We do 18s rRNA sequencing for Fungi or Yeast or Algal identification; selective 18S rRNA gene could be sequenced using the common ITS universal primers. For ~650 bps sequences proper combined contigs would be provided.

Our 18s rRNA sequencing services provides complete solution (DNA extraction, PCR amplification, Gel verification, Purification, Sequencing, Sequence assembling, Bioinformatics analysis (BLAST in Various Databases) and Construction of Phylogenetic tree. We also accept DNA samples, PCR products (Purified/Unpurified) for Sequencing. According to the samples charges may vary. For better result submit direct microbial culture plate or slant.

26s rRNA (D1/D2) Region Sequencing is used along with ITS region sequencing for the better identification. This service also available with us.

We provide below contents documents as a 18s rRNA Result:

  1. ITS Sequence raw data in different file format.
  • .ab1 - Sequence with chromatogram
  • .pdf - Sequence with Chromatogram
  • .txt - Only sequence
  • .fasta - Only Sequence
  • Assembled Sequence (Contig)
  • Blast result generated from All GenBank + NCBI+EMBL+DDBJ+PDB sequences.
  • Summary report comprises:
    • Methodology for DNA Extraction, PCR, Sequencing and Bioinformatic analysis.
    • Sequence analysis report contains sequence read Normal Length; Q16 & Q20 read length, GC Content details.
    • Proper contigs sequence with coverage image
    • Final Result
    • Phylogeny Tree.

We Provide all the bioinformatics analysis including Phylogenetic tree & Customer specific gene analysis with free of cost

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Customized Gene Panels

Starting at $35.00 per sample

Cutomized Gene study according to the Clients need.
DNA Extraction, Primer synthesis, PCR, Sequencing & Bioinformatics.

Cutomized Gene study according to the Clients need.
DNA Extraction, Primer synthesis, PCR, Sequencing & Bioinformatics.

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PCR

Starting at $3.00 per sample
Request a quote for more information about this service.

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Genomic DNA Extraction

Starting at $3.00 per sample

DNA Extraction NGS Grade. Assured Quality. Total Concentration 1ug to 4ug.

All Tybe of samples same charges for Extraction 

DNA Extraction NGS Grade. Assured Quality. Total Concentration 1ug to 4ug.

All Tybe of samples same charges for Extraction 

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Whole Genome Sequencing

Starting at $175.00 per sample

Illumina Sequencing

Data - 1 to 2 GB data 

Extraction + Library + Sequencing. Analysis not inculded

Illumina Sequencing

Data - 1 to 2 GB data 

Extraction + Library + Sequencing. Analysis not inculded

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Transcriptome Sequencing and Profiling

Starting at $175.00 per sample

Illumina Sequencing - Pair End

Extraction + Library + Sequencing (Analysis not included)

Data - 2GB data

Illumina Sequencing - Pair End

Extraction + Library + Sequencing (Analysis not included)

Data - 2GB data

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Metagenomic Sequencing

Starting at $80.00 per sample

Metagenomics is the study of variation of species in the genomes of whole microbial community. This study done with the genetic material recovered directly from environmental samples and in complex microbial samples. It covers the broad fields like environmental and eco genomics or community Genomics. In microbial diversity study,... Show more »

Metagenomics is the study of variation of species in the genomes of whole microbial community. This study done with the genetic material recovered directly from environmental samples and in complex microbial samples. It covers the broad fields like environmental and eco genomics or community Genomics. In microbial diversity study, it has opened up a new era. It has direct access to the genomes of various uncultivable microorganisms in their natural habitat. 16s rRNA and 18s rRNA sequence Long read of span, multiple variable regions, enabling high-resolution species identification.

We are providing integrated microbial Next Generation Sequencing services with very high quality data. In Fast turnaround time. The advanced microbial specialized automated systems providing efficient and fast sequencing workflow from the various sample sources. Different sequencing platforms are available for this study.

16s rRNA / 18s rRNA Sequencing:

Metagenomic 16s rRNA / 18s rRNA sequencing enables more efficient identification of bacterial diversity and archaeal microbial diversity present in a specific environments.

Whole Genome Shotgun Sequencing:

Metagenomic Whole Genome shotgun sequencing helps to evaluate and to detect the abundance of microbial diversity under various conditions. For this shotgun sequencing, first DNA is purified, and it is randomly sheared into smaller fragments before sequencing.

Benefits:

  • No need to culture the samples (Most microbes are non-culturable)
  • Studying the microbes in their native environments.
  • It helps in detecting novel microbes and genes from the environmental microbial community.
  • High-throughput reads and more accurate data.
  • More Economic and affordable Cost.

Best Suitable NGS Technology for this study:

  • MiSeq system
  • HiSeq2000/2500
  • Ion PGM
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SEM/EDS Scanning Electron Microscopy

Starting at $35.00 per sample

SEM Scanning Electron Microscopy

SEM Scanning Electron Microscopy

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GC-MS

Gas Chromatography Coupled Mass Spectrometry
Starting at $35.00 per run
Request a quote for more information about this service.

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UV-VIS Spectroscopy

Starting at $3.00 per sample
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UPLC

Ultra performance liquid chromatography
Starting at $15.00 per run
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HPLC

High Performance Liquid Chromatography
Starting at $15.00 per run
Request a quote for more information about this service.

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DNA Barcoding

Starting at $30.00 per sample

RBCL Gene Study
matK Gene Study

Plant Identification Service
DNA barcoding is the use of short DNA sequences (~650 bp) of the standard segment of the genome for large scale species identification. The Consortium for the Barcode of Life (CBOL) plant-working group recommended the 2-locus combination of rbcL and matK as... Show more »

RBCL Gene Study
matK Gene Study

Plant Identification Service
DNA barcoding is the use of short DNA sequences (~650 bp) of the standard segment of the genome for large scale species identification. The Consortium for the Barcode of Life (CBOL) plant-working group recommended the 2-locus combination of rbcL and matK as the standard plant barcode. These two regions of chloroplast DNA were chosen due to efficient recovery of quality sequences and high levels of species discrimination. We use the universal primers for amplification of matK and rbcL loci in different plant species. Success rate in PCR was higher for rbcL (88%) compared with matK (69%). Currently used universal primers of rbcL and matK are able to amplify many of the plant species they may fail in certain cases due to primer mismatch at the annealing site.

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Phylogenetic Analysis

Starting at $30.00 per sample

COI Gene Study

Our COI sequencing services provides complete solution (DNA extraction, PCR amplification, Gel verification, Purification, Sequencing, Sequence assembling, Bioinformatics analysis (BLAST in Various Databases) and Construction of Phylogenetic tree. We also accept DNA samples, PCR products (Purified/Unpurified)... Show more »

COI Gene Study

Our COI sequencing services provides complete solution (DNA extraction, PCR amplification, Gel verification, Purification, Sequencing, Sequence assembling, Bioinformatics analysis (BLAST in Various Databases) and Construction of Phylogenetic tree. We also accept DNA samples, PCR products (Purified/Unpurified) for Sequencing. According to the samples charges may vary. For better result submit direct sample (insect, Tissue etc.,) stored in 90% ethonol.

Amplification of the COI gene:

The extracted DNA is used as the template for PCR to amplify the COI gene sequence. Universal primers (LCO1490 / HCO2198) or specific primers complementary to a conserved region are used so that COI region can be amplified. The PCR amplification produces multiple copies of the target DNA sequence. The resulting product (~650bp) is used as the template for the sequencing.

DNA sequencing:

Since we know the length and terminal base of each fragment, the sequences of the bases can be determined.

DNA sequencing can be done by the common Sanger sequencing method.

We provide below contents documents as a COI Result:

  1. Sequence raw data (Forward and Reverse) in different file format.
    .ab1 - Sequence with chromatogram
    .pdf - Sequence with Chromatogram
    .txt - Only sequence
    .fasta - Only Sequence

  2. Assembled Sequence (Contig) - ~ 650bp

  3. Blast result generated from All GenBank + NCBI+EMBL+DDBJ+PDB sequences.

  4. Summary report comprises:
    Methodology for DNA Extraction, PCR, Sequencing and Bioinformatic analysis.
    Sequence analysis report contains sequence read Normal Length; Q16 & Q20 read length, GC Content details.
    Proper contigs sequence with coverage image
    Final Result
    Phylogeny Tree.
    Importance of Cytochrome c Oxidase subunit I gene (COI) study:

Cytochrome c Oxidase subunit I gene (COI), the gold standard in animal, insect DNA barcoding. Size of the Gene is 648-bp. This tool is used in the identification of species.

Taxonomy is an integral part of any biological study. Scientific studies involving a taxonomy must have an accurate identification. Morphology alone sometimes fails as an effective identifier of species. In cases of morphologically similar species, or where the specimens are derived from larval or juvenile life stages, the usefulness of traditional methods of comparative morphology could be limited. It is heavily dependent on specialists.

COI study is most widely used markers for population genetic and phylogeographical studies across the animal kingdom. COI is one of the three mitochondrial DNA encoded subunits of the respiratory complexIV, which is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. Its utility is further increased when showed that a ~650bp fragment of the COI gene could be an efficient identification tool for many species. The diversity in the amino acid sequences coded by the gene was sufficient to place species reliably into higher taxonomic classifications (from phyla to orders). In addition, they observed that diversity in nucleotide sequences of the same gene region allowed for the discrimination of closely related lepidopteran and avian species. Therefore, it was proposed that a DNA barcoding system for most, if not all, animal life could be based upon sequence diversity in COI.

Identification is done by matching the barcode profile with those found in the Gene database. It is the most frequently used tools for computing sequence similarity is the Basic Local Alignment Search Tool, or BLAST, found in the National Center for Biotechnology Information (NCBI) website and other Gene bank. After submitting the query, the sequence will be fed into the algorithm on the BLAST server and locally aligned to the similar sequences in the database. This alignment will be translated into scores that signify similarity of sequences. A specimen is identified if that sequence matches one in the barcode library. Otherwise, the sequence could either be a novel barcode sequence for a given species, usually as a geographical variant or new haplotype, or it belonged to an unknown species.

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Spectroscopy

Price on request
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Scanning Electron Microscopy (SEM)

Scanning Electron Microscopy services
Price on request
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DNA Synthesis and Probe Development

Price on request
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DNA Extraction and Purification

Price on request
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Genotyping and Gene Expression Assays

Price on request
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RT-PCR

Reverse transcription polymerase chain reaction
Price on request
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Pyrosequencing

Price on request
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DNA Services

Price on request
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Liquid Chromatography (LC)

Price on request
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RNA Sequencing

Price on request
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Nucleic Acid Services

Price on request
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DNA Sequencing

Price on request
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Biology

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DNA Fragment Analysis

Price on request
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Biochemistry & Molecular Biology

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Targeted Gene Sequencing

Price on request
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Microscopy

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Chromatography

Price on request
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PCR Primer Design

Price on request
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Imaging & Spectroscopy

Price on request
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Other Mass Spectrometry Methods

Price on request
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Next Generation Sequencing (NGS)

Price on request
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RNA Services

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Computational Modeling

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Chemistry and Materials

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Separation/Purification Services

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Metagenomics

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Analytical Chemistry Services

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Mass Spectrometry

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Spectrophotometry

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Electron Microscopy

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Yaazh Xenomics

Project Manager

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