1331587437000

X-ray Crystallography Core Facility

Jupiter, Florida, US

The X-ray Crystallography Core Facility of Scripps FL offers state-of-the-art equipment and resources to scientists inside and outside of the Scripps FL campus by providing crystallographic analysis of their chosen biological macromolecules or organic/ inorganic small molecules.

The core facility offers and operates as a full service core by performing protein crystallization, X-ray diffraction data collection (both in-house and at Argonne National Laboratory) and processing, phasing, crystallographic refinement, model building, and visualization.

The structural data obtained by the core will provide scientists with a wealth of information including but not limited to biological functions, 3D-folding, ligand binding (small molecule or protein), or mutational effect of target macromolecules of their interests.

X-ray Crystallography Core Facility has not listed any services.

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Bacterial Protein Expression
Price on request

Crystallization construct design, cloning, expression, and purification in bacterial expression systems.

  • Multiple construct design, cloning, and test expression (point/insertion/deletion mutations if necessary)

  • Cys disulfide bond protein (Ig domain proteins and cell surface proteins) expression

  • Co-expression of protein complexes

Crystallization construct design, cloning, expression, and purification in bacterial expression systems.

  • Multiple construct design, cloning, and test expression (point/insertion/deletion mutations if necessary)

  • Cys disulfide bond protein (Ig domain proteins and cell surface proteins) expression

  • Co-expression of protein complexes

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Protein Crystallization
Price on request

Gryphon crystallization setup for up to 3 drops/condition in a 96 well plate. Plates and reagent costs are included.

For macromolecular crystallization experiments, the core facility uses commercially available 960 crystallization conditions plus various optimization reagents whose combination can make millions of different... Show more »

Gryphon crystallization setup for up to 3 drops/condition in a 96 well plate. Plates and reagent costs are included.

For macromolecular crystallization experiments, the core facility uses commercially available 960 crystallization conditions plus various optimization reagents whose combination can make millions of different crystallization conditions. The equipment available for crystallization are:

  • a Minstrel III, an automatic plate handling and imaging system,
  • two RoboIncubators, which can store crystallization plates in a temperature and humidity controlled environment,
  • a Leica stereomicroscope and a high powered stereoscope for manual crystal photography, crystal screening, and crystal manipulation.

The Innovadyne 96+8 crystallization robot and Emerald’s Matrix Maker can be used to perform automated protein crystallization and optimization experiments.

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X-Ray Diffraction
Price on request

Single crystal diffraction data collection and crystal screening.

  • Not-to-exceed quote available.

Single crystal diffraction data collection and crystal screening.

  • Not-to-exceed quote available.
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X-ray crystallography
Price on request

Small molecule / Macromolecules / Macromolecular complexes

  • Data reduction, phasing, model building and structure refinements.

  • Not-to-exceed quote available.

Small molecule / Macromolecules / Macromolecular complexes

  • Data reduction, phasing, model building and structure refinements.

  • Not-to-exceed quote available.

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Protein Services
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Biology
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Protein Expression
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Chemistry and Materials
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Material Characterization Services
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Analytical Chemistry Services
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Biochemistry & Molecular Biology
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Structural Biology
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