Elabscience Biotechnology Co.,Ltd(Elabscience) is a high-tech biotech corporation, specializing in reagents of immunodiagnostic technology.
With energetic and experienced research teams, professional laboratories, first-class equipment and material supplied from top bio-tech corporations, Elabscience is committed to developing and producing a large selection of innovative reagents of high quality to provide the Life Science research and diagnostic with a comprehensive, high-quality protein related products.
By application of innovative technique and combinative use of immunology, molecular biology and cell biology, our research subjects cover many areas, mainly involving vitro diagnosis, molecular markers, protein expression, food safety, drug residue, and environment protection.
Moreover, we have established seven technical service platforms , including ELISA Customized Service, Immunoassay service, Molecular Biology Service, Protein Expression and Purification Service, Antibody Preparation Service, Cell Culture Service and Whole Research Project Service， to provide one-stop experimental service with professional knowledge, facilities and attitude.
Elabscience has the top technology for antibody service and professional technicists of antibody, can meet all of the customer's needs for antibodies with one-stop service. The antibody services we provide include: expression of recombinant proteins, design and synthesis of polypeptide antigens, Animals immunization, Splenocyte fusions and subclone, ascites production and purification, antibody modification, antibody purification, immunoassay, etc..
Custom Polyclonal Antibodies:
1.Elabscience synthesis antigen $5,600.00
2. Custom Small Molecule Antigenic Antibody. $7,500.00
3. Monoclonal Antibody with the Same Antibody and Different Sites $12,000.00
The price includes shipping and handling charges. Pre-immune bleed, antibody and antigen are shipped together to the customer in 1 package at the end of project.
Packages can be customized upon customer request
Are you tired of subcloning?
Elabscience provides PCR cloning and subcloning services to save your energy and time from routinely subcloning for more creative research. Our subcloning service is highly flexible to any specific project requirements. The starting materials can be plasmid DNA or bacterial culture. Please contact our customer service for other starting materials. Our typical turnaround is 14 working days, the concrete time depending on the nature of the subcloning project.
Sample Submission Requirements:
Plasmid DNA, and bacterial culture are all acceptable. Other starting material, please contact our customer service. The following things are necessarily to be provided:
Our typical turnaround is in about 14 working days, the concrete time depending on the complexity of the subcloning project. Our package is as following:
Our service for Open Reading Frame (ORF) cloning offers a quick means to turn DNA sequences into expression-ready clones, thus greatly accelerate your biological research.
Our typical turnaround time is about 10 business days for genes up to 1 kb. Our typical delivery package includes the following items:
Site-directed mutagenesis is central to molecular biology, facilitating research in a variety of fields, including studies of gene regulatory elements, DNA-protein interaction, protein structure/function, enzyme active sites, and novel proteins. Elabscience's site-directed mutagenesis services, powered by sophisticated gene synthesis technology and high performance polymerase, include point mutations, deletions, and insertions. They can be customized to fit any scientific applications.
Sample Submission Requirements:
Our typical turnaround is in about 10 working days.
Our package is as following:
Cell proliferation detection
Using transfection, infection or staining techniques, we label fluorescence molecular to cells，then taking a high-resolution image of these cells to get more comprehensive information. The number of cells in different groups will be calculated by analytical processing software.
Elabscience has the top technology for antibody service and professional technicists of antibody, can meet all of the customer's needs for antibodies with one-stop service. The antibody services we provide include: expression of recombinant proteins, design and synthesis of polypeptide antigens, production of polyclonal antibodies, production of monoclonal antibodies, antibody modification, antibody purification, immunoassay, etc..
Different species available: mouse, rabbit, goat.
Custom Polyclonal Antibodies:
1.Use polypeptide as the antigen: Elabscience synthesize the peptide segment, and each peptide is respectively immune to two rabbits after coupling carrier and respectively uses antigenic affinity purification, about 3mg polyclonal antibodies can be obtained.
a. each antigen uses 1 peptide: $1200.00
b. each antigen uses 2 peptides: $2200.00
c. each antigen uses 3 peptides: $3000.00
Three-peptides Polyclonal Antibody - Over 95% success rate of WB detection of nature protein
2.Fusion protein purification and antibody production $3000
3. Custom phosphor-peptide antibody production $2500
We synthesis Peptide (Less than 15 amino acids, purity＞85%, 15 mg phosphorylated peptides and 15 mg nonphosphorylated peptides)
4. Custom Small Molecule Antigenic Antibody. Inquire price
The price include shipping and handling charges. Pre-immune bleed, antibody and peptide are shipped together to the customer in 1 package at the end of project.
Packages can be customized upon customer request
The research of cell mobility is wildly used in the tumor invasion and metastasis. Tumor metastasis is one of distinct features of malignant tumor biology. Cancer cells manifest strong mobility during certain stage of cancer metastasis. For example, cancer cells break away from original foci, invading into neighboring tissue and passing through vessel wall into blood circulation, then thread out of the vessel into secondary position. Therefore, research about cell’s mobility exerts great importance in comprehending the mechanism of cancer cell’s invasion and transfer.
Cell scratch is one of method to assay tumor cell’s mobility. It takes reference from in vitro cell wound healing model, making scratch injury on the monolayer cells cultivated in vitro, then observing the mobility of cancer cells by means of medication or interference.
Put Transwell chamber into Culture Plate (inboard chamber is called upper chamber and inboard Culture Plate is called lower chamber). Upper and lower chamber are separated by a polycarbonate membrane. We plant cells in the upper chamber. As polycarbonate equips permeability, components in the lower culture medium can affect cells of the upper chamber. Therefore, we can study the impact of components in the lower medium on the cell’s growth and mobility. Commonly used two types of this experiment are regular transwell chamber(transfer) and matrix paved transwell chamber(invasion).
Stable Cell Line Services for Assay Development
In vitro assays based on gene engineered cells play crucial roles in modern drug discovery processes. Especially for GPCRs and ion channels, multiple trans-membrane proteins, the cell-based assays are dominant in chemical library screening. There are many stable cell lines and assay techniques are developed to model human bodies. With expertise on cell line generation and assay development, Elabscience offers your Gene-to-Assay solution for your drug discovery project.
Elabscience has developed a Ready-to-Assay GPCR & Ion Channel Cell Line Collection which is available as product for your order. If you can’t find the stable cell lines of your interests, please choose the service to customize your stable cell lines with our available assays or other assays upon your specifications.
Elabscience utilizes 2-round selection strategy for GPCR and ion channel stable cell line generation upon different applications. To reduce the cost and timeline, Elabscience chooses HTS-compatible fluorescence- or luminescence-based assays to screen stable clones in the 1st round selection. In 2nd round selection, radioisotope- and electrophysiology-based assays are available upon your applications. For nuclear receptors, transporters, and other targets which have no ready HTS-compatible assays, Elabscience offers mRNA or protein level assays as alternative solutions.
In addition, Elabscience can develop a new assay upon your specifications. Please feel free to contact us for technique support.
Flow cytometric detection
Flow cytometry is a laser based, biophysical technology employed in cell counting, sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second.
Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. A common variation is to physically sort particles based on their properties, so as to purify populations of interest.
Years of solid experience and unique expertise in custom protein expression and purification services render Elabscience to help you break the bottlenecks in protein expression and purification. Our streamlined services can take your project directly from gene synthesis to protein expression, refolding, purification, and stable cell line generation. In addition to a wide range of choices in multiple protein expression systems, our superior protein purification services and large-scale production capacity can meet your needs in all aspects.
Protein Purification and Characterization
Elabscience offers several methods of protein purification and characterization, allowing customers to choose the ideal level of purity for the intended final application. Each of these options is available for both small and large amounts of protein. Elabscience routinely maintains stringent QC and SOPs on its protein purification process. In addition, before shipping, our protein expression experts characterize each target protein to assure that it meets the customer's stated requirements.
Protein Purification Options
Chromatography: Ion-Exchange Hydrophobic Interaction Affinity Size Exclusion
Purification Tags: 6xHis GST MBP FLAG
Protease for Tag Removal: Thrombin Enterokinase rTev PreScission Factor Xa
Electrophoresis and Coomassie staining of SDS-PAGE
Protein concentration determination
Recombinant Protein Services
Elabscience's custom recombinant protein services provide a comprehensive portfolio of protein expression systems, including bacteria, yeast, baculovirus infected insect cells, and mammalian cells. We offer a full spectrum of protein purification methods to achieve the ideal level of protein purity for your downstream applications. With customized solutions, flexible production scale, and proprietary technologies, we work in partnership with our clients to ensure efficient and high quality reagent protein production, process development and scale-up production of biologics for research purposes.
Elabscience has delivered over 2,000 batches of recombinant proteins used for functional assays, high throughput screens, antigen generation, structural analysis, and many other drug discovery applications. When our extensive expertise in recombinant proteins is integrated with our in-house gene synthesis and assay development capabilities, we can quickly deliver your projects in a flexible and cost-effective manner. Moreover, our dedicated technical account managers will guide your project through every step of the process, and constantly keep you informed of the project progress
Bacterial Expression System
Bacterial Protein Expression System is the most common and economical mean of protein expression available today.
Competitive price: starting from $1,550
Fast turnaround: as little as 4 weeks
Flexible scale-up protein production (from 10 L to 1,000 L)
Guaranteed Package: 3 mg of purified proteins for $1,980
One-stop service platform: Elabscience can take your project directly from gene synthesis to downstream activities including recombinant protein expression, stable cell line generation, antibody production, and assay development.
Yeast Expression System
Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
Proprietary Technologies: Our Technology considers promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location, which are essential for high-level recombinant protein production.
Humanized antibody production: Elabscience has developed a platform that produces humanized antibodies in yeast expression system. The similarity between the yeast and mammalian system for recombinant antibody expression enables Elabscience to reduce the high cost associated with manufacturing recombinant antibody in mammalian expression system.
Streamlined Services: Elabscience can take your project directly from gene synthesis, to downstream activities including recombinant protein expression, stable cell line generation, antibody production, and assay development.
Transient Protein Expression Service
Mammalian cells have the ability to perform most comprehensive post-translational modifications and to secrete glycoproteins that are correctly folded and contain complex antennary oligosaccharides with terminal sialic acid. Mammalian cells are widely used in the production of recombinant proteins, antibodies, virus, viral-subunit proteins, and gene-therapy vectors. Large-scale and fast mammalian transient system for protein expression is of great concerns to researchers conducting drug discovery projects.
Elabscience's transient protein expression service promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level. Our proprietary mammalian expression system platform can successfully be used for cases in which the folding of the proteins is crucial to the customers' intended applications.
Large-scale Protein Production Service
With the rapid growth of the protein drug market and recent advances in proteomics, the supply of large quantities of pure, active proteins has become essential for the production of protein drugs and to the development of drug assays and research methods. Elabscience is fully equipped to meet this need for large-scale protein production service. Our versatile and reliable protein expression system, which includes fermentors, wave mixers, and bioreactors, combined with our large-scale purification service, allows Elabscience to provide its clients in both the public and private research sectors, academia, and all levels of the pharmaceutical industry with protein orders as large as grams. Elabscience has delivered more than 2000 proteins, including 240 grams of protein on a single project.
Customized Protein Service
Researchers are often baffled by the expression of the target proteins. Certain categories of proteins are extremely difficult to express, such as membrane proteins, toxins, and ion channel proteins. The problem tackling process is labor-intensive and time-consuming as there are tens of factors to consider for protein expression and purification.
Elabscience is providing cutting-edge solutions to these challenges with the latest strategies from experienced researchers who have successfully delivered more than 2,000 proteins using our advanced bacterial expression system and our proprietary protein refolding technology. Elabscience has also developed a wide array of solutions to produce highly purified bioactive proteins for drug discovery, structural biology and other biological studies. Over 90% customer ordered recombinant proteins are successfully expressed and purified at Elabscience using our bacterial expression system.
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