Wuhan Elabscience Biotechnology Co., Ltd

Are you tired of subcloning?

Elabscience provides PCR cloning and subcloning services to save your energy and time from routinely subcloning for more creative research. Our subcloning service is highly flexible to any specific project requirements. The starting materials can be plasmid DNA or bacterial culture. Please contact our customer service for other starting materials. Our typical turnaround is 14 working days, the concrete time depending on the nature of the subcloning project.

Sample Submission Requirements:

Plasmid DNA, and bacterial culture are all acceptable. Other starting material, please contact our customer service. The following things are necessarily to be provided:

• 1. Complete sequence of the template including target gene and its vector
  
• 2. Maps and antibiotic resistance of the template and destination vectors.
  
• 3. Recommended to send 2 ug of template DNA as starting material. If the starting material is bacterial culture, please send the culture in sufficient amount.
  

Delivery Specifications:

Our typical turnaround is in about 14 working days, the concrete time depending on the complexity of the subcloning project. Our package is as following:

• 1.4 ug lyophilized destination vector containing the gene
  
• 2.Sequence chromatograms covering your gene (electronic)
  
• 3.Construct map for the plasmid (electronic)
  
• 4.Quality assurance certificate

Our service for Open Reading Frame (ORF) cloning offers a quick means to turn DNA sequences into expression-ready clones, thus greatly accelerate your biological research.

Key Features:

• 1. Time-saving service for ORF cloning in your customized vector at the best price
  
• 2. One-stop platform including downstream services for protein expression and functional analysis
  

Service Specifications:

• 1. Sequence Confirmation: Your gene of interest will be sequence validated base-by-base against the complete ORF sequence in the database.
  
• 2. De Novo Synthesis:
  
• 3. Cloning Vector: Your gene of interest can be cloned into any commercial or non-commercial vector of your choice.
  
• 4. Quality Control: Every customized vector with your ORF gene of interest is strictly inspected before delivery to ensure 100% accuracy.
  

Delivery Specifications:

Our typical turnaround time is about 10 business days for genes up to 1 kb. Our typical delivery package includes the following items:

• 1. 10 ug lyophilized customized vector containing the gene of your interest
  
• 2. Sequencing data of each clone (electronic version)
  
• 3. Construct map for the plasmid
  
• 4. Quality assurance certificate

Site-directed mutagenesis is central to molecular biology, facilitating research in a variety of fields, including studies of gene regulatory elements, DNA-protein interaction, protein structure/function, enzyme active sites, and novel proteins. Elabscience's site-directed mutagenesis services, powered by sophisticated gene synthesis technology and high performance polymerase, include point mutations, deletions, and insertions. They can be customized to fit any scientific applications.

Advantages:

• 1. Unparalleled Accuracy: Elabscience's high performance polymerase ensures accuracy of mutants, avoiding creation of unwanted mutations.
  
• 2. Unlimited Sites: Using Elabscience sophisticated mutagenesis technology, mutations can be introduced to you at any site.
  
• 3. Mutation on Large Construct: Our technology is optimized to introduce mutations, insertions, and deletions on constructs as large as up to 12 kb (including target gene and its vector).
  
• 4. Comprehensive Service Package: Elabscience offer comprehensive upstream and downstream services, including template sequencing, expression vector construction, and protein expression and purification
  
• 5. Most Competitive Price
  

Sample Submission Requirements:

• 1. The complete sequence of the template including the target gene and its vector
  
• 2. Mutation specifications
  
• 3. Maps and antibiotic resistance of the template and destination vectors
  
• 4. Recommended to send 4 ug of template DNA as the starting material. If the starting material is bacterial culture, please send the culture at sufficient amount.
  

Delivery Specifications:

Our typical turnaround is in about 10 working days.

Our package is as following:

• 1. 4 ug lyophilized destination vector containing the gene
  
• 2. Sequence chromatograms covering your gene (electronic)
  
• 3. Construct map for the plasmid (electronic)
  
• 4. Quality assurance certificate

Cell proliferation detection

Using transfection, infection or staining techniques, we label fluorescence molecular to cells，then taking a high-resolution image of these cells to get more comprehensive information. The number of cells in different groups will be calculated by analytical processing software.

Elabscience has the top technology for antibody service and professional technicists of antibody, can meet all of the customer's needs for antibodies with one-stop service. The antibody services we provide include: expression of recombinant proteins, design and synthesis of polypeptide antigens, production of polyclonal antibodies, production of monoclonal antibodies, antibody modification, antibody purification, immunoassay, etc..
Different species available: mouse, rabbit, goat.

Custom Polyclonal Antibodies:
1. Peptide synthesis (Less than 15 amino acids, purity＞85%, 10 mg:5mg used for immunization, 5 mg used for purification) or Obtaining or synthesizing genes and expressing proteins. 4.5~5 mg proteins (2~2.5 mg used for immunization, 2.5 mg used for purification)
2. Conjugate peptide to KLH/BSA/OVA or Protein purification and identification
3.Animals immunization (2 rabbits), Pre-immune bleed from 2 rabbits，immunization for 4 times
4.Final serum collection
5.Affinity purification(3-5mg)
6.ELISA assay
7. Termination of host animal AFTER collecting serum from Rabbit

Turn-around time:3-4m

Prices:
1.Use polypeptide as the antigen: Elabscience synthesize the peptide segment, and each peptide is respectively immune to two rabbits after coupling carrier and respectively uses antigenic affinity purification, about 3mg polyclonal antibodies can be obtained.
a. each antigen uses 1 peptide: $1200.00
b. each antigen uses 2 peptides: $2200.00
c. each antigen uses 3 peptides: $3000.00
Three-peptides Polyclonal Antibody - Over 95% success rate of WB detection of nature protein
2.Fusion protein purification and antibody production $3000
3. Custom phosphor-peptide antibody production $2500
We synthesis Peptide (Less than 15 amino acids, purity＞85%, 15 mg phosphorylated peptides and 15 mg nonphosphorylated peptides)
4. Custom Small Molecule Antigenic Antibody. Inquire price

The price include shipping and handling charges. Pre-immune bleed, antibody and peptide are shipped together to the customer in 1 package at the end of project.
Packages can be customized upon customer request

Cell transfer

The research of cell mobility is wildly used in the tumor invasion and metastasis. Tumor metastasis is one of distinct features of malignant tumor biology. Cancer cells manifest strong mobility during certain stage of cancer metastasis. For example, cancer cells break away from original foci, invading into neighboring tissue and passing through vessel wall into blood circulation, then thread out of the vessel into secondary position. Therefore, research about cell’s mobility exerts great importance in comprehending the mechanism of cancer cell’s invasion and transfer.

Cell scratch

Cell scratch is one of method to assay tumor cell’s mobility. It takes reference from in vitro cell wound healing model, making scratch injury on the monolayer cells cultivated in vitro, then observing the mobility of cancer cells by means of medication or interference.

Induced transition

Put Transwell chamber into Culture Plate (inboard chamber is called upper chamber and inboard Culture Plate is called lower chamber). Upper and lower chamber are separated by a polycarbonate membrane. We plant cells in the upper chamber. As polycarbonate equips permeability, components in the lower culture medium can affect cells of the upper chamber. Therefore, we can study the impact of components in the lower medium on the cell’s growth and mobility. Commonly used two types of this experiment are regular transwell chamber(transfer) and matrix paved transwell chamber(invasion).

Stable Cell Line Services for Assay Development

In vitro assays based on gene engineered cells play crucial roles in modern drug discovery processes. Especially for GPCRs and ion channels, multiple trans-membrane proteins, the cell-based assays are dominant in chemical library screening. There are many stable cell lines and assay techniques are developed to model human bodies. With expertise on cell line generation and assay development, Elabscience offers your Gene-to-Assay solution for your drug discovery project.

Elabscience has developed a Ready-to-Assay GPCR & Ion Channel Cell Line Collection which is available as product for your order. If you can’t find the stable cell lines of your interests, please choose the service to customize your stable cell lines with our available assays or other assays upon your specifications.

Service Package

Elabscience utilizes 2-round selection strategy for GPCR and ion channel stable cell line generation upon different applications. To reduce the cost and timeline, Elabscience chooses HTS-compatible fluorescence- or luminescence-based assays to screen stable clones in the 1st round selection. In 2nd round selection, radioisotope- and electrophysiology-based assays are available upon your applications. For nuclear receptors, transporters, and other targets which have no ready HTS-compatible assays, Elabscience offers mRNA or protein level assays as alternative solutions.

In addition, Elabscience can develop a new assay upon your specifications. Please feel free to contact us for technique support.

Flow cytometric detection

Flow cytometry is a laser based, biophysical technology employed in cell counting, sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second.

Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. A common variation is to physically sort particles based on their properties, so as to purify populations of interest.

Years of solid experience and unique expertise in custom protein expression and purification services render Elabscience to help you break the bottlenecks in protein expression and purification. Our streamlined services can take your project directly from gene synthesis to protein expression, refolding, purification, and stable cell line generation. In addition to a wide range of choices in multiple protein expression systems, our superior protein purification services and large-scale production capacity can meet your needs in all aspects.

Protein Purification and Characterization

Elabscience offers several methods of protein purification and characterization, allowing customers to choose the ideal level of purity for the intended final application. Each of these options is available for both small and large amounts of protein. Elabscience routinely maintains stringent QC and SOPs on its protein purification process. In addition, before shipping, our protein expression experts characterize each target protein to assure that it meets the customer's stated requirements.

Protein Purification Options

• Chromatography: Ion-Exchange Hydrophobic Interaction Affinity Size Exclusion

• Purification Tags: 6xHis GST MBP FLAG

• Protease for Tag Removal: Thrombin Enterokinase rTev PreScission Factor Xa

• Protein Characterization:

Electrophoresis and Coomassie staining of SDS-PAGE
Protein concentration determination
Western blot
Absorption spectrum
HPLC
Mass Spec
MALDI-TOF
Size-Exclusion Chromatography
N-terminal sequencing

Recombinant Protein Services

Elabscience's custom recombinant protein services provide a comprehensive portfolio of protein expression systems, including bacteria, yeast, baculovirus infected insect cells, and mammalian cells. We offer a full spectrum of protein purification methods to achieve the ideal level of protein purity for your downstream applications. With customized solutions, flexible production scale, and proprietary technologies, we work in partnership with our clients to ensure efficient and high quality reagent protein production, process development and scale-up production of biologics for research purposes.

Elabscience has delivered over 2,000 batches of recombinant proteins used for functional assays, high throughput screens, antigen generation, structural analysis, and many other drug discovery applications. When our extensive expertise in recombinant proteins is integrated with our in-house gene synthesis and assay development capabilities, we can quickly deliver your projects in a flexible and cost-effective manner. Moreover, our dedicated technical account managers will guide your project through every step of the process, and constantly keep you informed of the project progress

Bacterial Expression System

Bacterial Protein Expression System is the most common and economical mean of protein expression available today.

Advantages:

• Competitive price: starting from $1,550

• Fast turnaround: as little as 4 weeks

• Flexible scale-up protein production (from 10 L to 1,000 L)

• Guaranteed Package: 3 mg of purified proteins for $1,980

• One-stop service platform: Elabscience can take your project directly from gene synthesis to downstream activities including recombinant protein expression, stable cell line generation, antibody production, and assay development.

Yeast Expression System

Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins.

Advantages

• Proprietary Technologies: Our Technology considers promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location, which are essential for high-level recombinant protein production.

• Humanized antibody production: Elabscience has developed a platform that produces humanized antibodies in yeast expression system. The similarity between the yeast and mammalian system for recombinant antibody expression enables Elabscience to reduce the high cost associated with manufacturing recombinant antibody in mammalian expression system.

• Streamlined Services: Elabscience can take your project directly from gene synthesis, to downstream activities including recombinant protein expression, stable cell line generation, antibody production, and assay development.

Transient Protein Expression Service

Mammalian cells have the ability to perform most comprehensive post-translational modifications and to secrete glycoproteins that are correctly folded and contain complex antennary oligosaccharides with terminal sialic acid. Mammalian cells are widely used in the production of recombinant proteins, antibodies, virus, viral-subunit proteins, and gene-therapy vectors. Large-scale and fast mammalian transient system for protein expression is of great concerns to researchers conducting drug discovery projects.

Elabscience's transient protein expression service promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level. Our proprietary mammalian expression system platform can successfully be used for cases in which the folding of the proteins is crucial to the customers' intended applications.

Large-scale Protein Production Service

With the rapid growth of the protein drug market and recent advances in proteomics, the supply of large quantities of pure, active proteins has become essential for the production of protein drugs and to the development of drug assays and research methods. Elabscience is fully equipped to meet this need for large-scale protein production service. Our versatile and reliable protein expression system, which includes fermentors, wave mixers, and bioreactors, combined with our large-scale purification service, allows Elabscience to provide its clients in both the public and private research sectors, academia, and all levels of the pharmaceutical industry with protein orders as large as grams. Elabscience has delivered more than 2000 proteins, including 240 grams of protein on a single project.

Customized Protein Service

Researchers are often baffled by the expression of the target proteins. Certain categories of proteins are extremely difficult to express, such as membrane proteins, toxins, and ion channel proteins. The problem tackling process is labor-intensive and time-consuming as there are tens of factors to consider for protein expression and purification.

Elabscience is providing cutting-edge solutions to these challenges with the latest strategies from experienced researchers who have successfully delivered more than 2,000 proteins using our advanced bacterial expression system and our proprietary protein refolding technology. Elabscience has also developed a wide array of solutions to produce highly purified bioactive proteins for drug discovery, structural biology and other biological studies. Over 90% customer ordered recombinant proteins are successfully expressed and purified at Elabscience using our bacterial expression system.