Wax-it Histology Services, Inc.

Validate and optimize protocols for an extensive list of antibodies.

Deliverables:
- Cover-slipped stained slide

Turnaround time: 5-10 Business Days
-NOTE: turn around time will vary for antibodies requiring optimization of protocol

Deliverables: Cover-slipped H&E Stained slide
Turn around time: 5-10 Business Days

Add on services:
- Processing of formalin-fixed wet tissue
- Embedding of formalin-fixed wet tissue
- Sectioning of paraffin blocks to mount on unstained slides. Unstained slides can be stained.
- Scanning of stained slides at 20x or 40x
- Pathology Report of stained slides by in-house pathologist

Implanted Medical Device Cutting & Grinding

Our newly developed cutting & grinding protocol can be used to obtain 10um sections of tissue implanted with a medical device.

Advantage of this technique: Tissue implanted medical device does not have to be removed to obtain section. Therefore, the direct effects of device on surrounding tissue can be observed without the risk of damaging tissue during device removal.

Deliverables:
-Tissue implanted Medical Device thinly sectioned and mounted onto a slide (IHC staining available upon request)

Turnaround time: 5-10 Business Days

Add on services:
- Staining of slides
- Whole slide imaging of slide at 20x or 40x

List of special stains offered:
• Alcian Blue
• Astra Blue – Vital New Red
• Chloroacetate Esterase
• Congo Red
• Wright Giemsa Stain
• Goldner's Trichrome
• Luxol Fast Blue – Cresyl Violet
• Masson's Trichrome
• Movat’s Pentachrome
• Mayer’s Mucincarmine stain
• Myosin ATPase at PH 4.2, 4.6, 9.4
• Oil-Red-O
• Periodic Acid Shieff (PAS)
• Phloroglucinol stain
• Picrosirius red (PSR)
• Prussion Blue
• Ruthenium Red for Pectin
• Safranin O and Fast Green
• Silver Stain
• Toluidine Blue
• Toluidine Blue Phloroglucinol Plant Stain
• TUNEL
• Verhoeff's Van Gieson
• Wright's Stain

We are happy to accommodate your special requests for stains not found on our list. We are also willing to work together with you to develop or modify protocols to achieve specific goals with your research.

Deliverables: Special stained slide
Turnaround time: 5-10 Business Days (Rushed Service available upon request)

We will gross, paraffin process and embed fixed tissue into a paraffin blocks.

Deliverables: Paraffin Block
Turnaround time: 5-10 Business Days (Rushed services available upon request)

Fixed wet tissue soaked in ethanol or formalin and pre-grossed tissue in cassettes can be submitted for this service. Client can determine embedding orientation based on region of interest.

Sectioning of paraffin blocks and mounted onto slides. Slides can be stained or left unstained.

Deliverables:
-Unstained slides
-Slides can be stained upon request

Turnaround time: 5-10 Business Days (Rushed service available upon request)

We have a rich experiences working with various implantable biomedical devices. Our dedicated specialists are ready to chat with you, to learn more about your research and to make suggestions along the way.

Embed tissue into OCT block, ready to be used for cryosectioning.

Deliverables:
-Frozen blocks

Turnaround time: 5-10 Business Days (Rushed service available upon request)

We have internally developed protocols for methylmethacrylate (MMA) and we are happy to work with your request for glycolmetharcylate (GMA), epoxy and other type of resin materials.

All stained slides can be imaged at 20x ($20 per slide) or 40x ($40 per slide).

Deliverables:
-FTP Link of images on the server
-DVD of images

Turnaround time: 5-10 Business Days (Rushed Services Available Upon Request)

Wax-it provides a full package of immunofluorescence services. Our specialists are ready to sit down with you to learn about your goals and to provide assistance and recommendations along the way.

Our state-of-art confocal platform allows for specific detection up to four fluorescence probe simultaneously with minimum photo-bleaching.

Our pathologists will work with you to determine an appropriate scoring criteria, and if you wish Wax-it can generate a pathology report for you: quantified data can be collated into a presentation-ready report or power point presentation, while images can be professionally edited for publication-ready submission.