Washington Biotechnology, Inc.

WBI has a wide variety of animal models for inflammation and inflammatory diseases.
These include:

Carrageenan Model – Rat 1-day assay
Irritant-induced Inflammation Models – Mouse or Rat
2-week assay
Air Pouch Model – Rat or Mouse 6-day assay

Data analysis with either model includes the basic paw swelling measurement supplemented as requested with chemistries, cell counts, cytokine measurements and histopathological analysis of bone at affected joints.

These models are useful in the initial in vivo evaluation of anti-inflammatory compounds developed for the treatment of diseases such as rheumatoid arthritis and multiple sclerosis. The basic measurement in the first two models is measurement of edema in response to irritants. In the third model, the production of cytokines is measured in response to irritation with or without drug treatment.

Test materials are administered by injection, oral, or intratracheal administration. Plasma or sera are collected at designated time intervals for prompt shipment to your laboratory.

Our In Vivo tests for analgesic effects of test materials include mouse and rat models for thermal, mechanical, and chemical stimuli (1). Basic models for thermal and mechanical pain responses may also be combined with inflammation models to provide information on analgesic activity in inflamed tissue.

Formalin Test (Chemical Sensitivity)

Hargreaves Test (Thermal Sensitivity)

Von Frey Fibers (Mechanical Sensitivity)

We have the facilities to culture many different types of pathogenic bacteria for the evaluation of the efficacy of potential antibiotics in rodent models. The bacteria are administered by various routes to model a variety of disease states from food poisoning to lung infection to burn infections. Assay end points include bacterial counts in blood samples and in selected tissues.

Test materials are administered by injection, oral, or intratracheal administration. Plasma or sera are collected at designated time intervals for prompt shipment to your laboratory.

Our necropsy services include removal, weights, preservation and collection of designated organs for histology or shipment to your laboratory.

WBI models for test material anti-infection efficacy

Mouse models for survival, bacterial counts (blood and organs), body weights and necropsy.

Full statistical analysis

• Candida albicans
  
• Listeria monocytogenes
  
• Methicillin Resistant (MRSA) and Methicillin Sensitive
  (MSSA) Staph Aureus
  
• Pseudomonas aeruginosa
  
• Salmonella
  
• Vibrio cholera
  

Custom model development services for bacterial infection studies.

Streptozocin-induced Diabetes - Rat 30-day assay

Rats treated with the antibiotic Streptozocin provide our model for studying the effectiveness of potential anti-diabetic agents. Diabetic condition in each rat is assessed by blood glucose measurements to select rats for the treatment of Test Materials. The treatment study parameters include urine collection studies with assay for albumin excreted in urine to provide a Urinary Albumin Excretion Rate (UAER). Ancillary measurements include glucose, ketones and blood in urine. We provide a statistical analysis of the UAER data in our Final Report along with the glucose and other measurements used to select diabetic rats for treatment.

Test materials are administered by injection, oral, or intratracheal administration. Plasma or sera are collected at designated time intervals for prompt shipment to your laboratory.

Our necropsy services include removal, weights, preservation and collection of designated organs for histology or shipment to your laboratory.

A number of cell culture assays provide valuable insights into the efficacy of anti-inflammatory drugs in prevention of cytokine expression. We measure the effect of your drug on cytokine production in a cell culture model for inflammation.

We are experienced with both the basic and extended protocols of immune response screens.

In the basic immune response screen, Swiss Webster mice or Sprague-Dawley rats are used to evaluate the immunogenicity of the test material over a 38-day period.

single dose, multi-dose, infusion - mouse, rat, hamster, rabbit, guinea pig

Collagen-Induced Arthritis (CIA) - Mouse or Rat 45-day assay

Pristane-Induced Arthritis (PIA) - Rat 30-day assay

Adjuvant-Induced Arthritis (AIA) - Rat 30-day assay

Ovalbumin-Induced Arthritis - Rabbit 30-60 day assay

PG-PS 10S - Rat 22-day assay

In addition to the basic assay measurements of paw edema, valuable supplementary data can be obtained through:

Clinical chemistries on plasma samples
Complete cell counts and differential cell counts on blood samples
Cytokine measurements
Histopathological analysis of affected joints
Test materials are administered by injection, oral, or intratracheal administration. Plasma or sera are collected at designated time intervals for prompt shipment to your laboratory.
Our necropsy services include removal, weights, preservation and collection of designated organs for histology or shipment to your laboratory.

Non-GLP toxicity studies.
Our experienced technical staff will provide expert interpretation of results for:

• Acute toxicity: single-dose range finding studies (mice, rats)
  
• Repeat dose toxicity: multiple-dose range finding studies (mice, rats) *
  
  • Repeat dose studies can be tailored to your disease
    
• model dosing regimen
  
• Maximum tolerated dose
  
• Blood chemistry: liver enzymes analysis; complete chemistry panel
  
• CBC/differentials: analysis of blood samples for cell counts within 24 hours
  
• Organs: collections, weights, preservation (formalin or flash-frozen)
  
• Histopathology: tissue sections: H&E or T-blue staining; Pathology Report
  
• Other animal species: Rabbits, guinea pigs, hamsters

Xenograft Models in Immunodeficient Nude Mouse or Rat

Efficacy Tests for Potential Anti-Cancer Agents (compounds, proteins, vaccines)
• Our experienced personnel provide quality results and skilled observations.
• Cost-efficient studies with rapid scheduling.
• Data transmitted promptly to Sponsor during dosing/measurement phases.
• Your Project is described in detail – Custom Protocol, Final Report including statistical/graphical analysis of data.
• All of our in vivo studies and cell cultures are carried out at our facility in Baltimore, Maryland, U.S.A.
• All animal species are acquired from established U.S. laboratory animal suppliers (Jackson Laboratories, HSD, CRL or Taconic).

We provide expert services, rapid project start-up and competitive prices for:
- Maximum Tolerated Dose (MTD) for Toxicity Information & Dosing Recommendation
- Subcutaneous Xenograft Models
- Orthotopic Models
- Syngeneic Models
- Custom Model Development

Analysis of Endpoints
- Primary tumor growth: reported weekly with tabulated and graphed results
- Metastasis
- Metastatic growth
- Tumor growth recovery post-dosing
- Mortality
- Observations for toxic effects
- Recovery of tumors: Preservation by flash-freezing, formalin or paraffin.

Routes of Administration for Test Compounds or Vaccines
- Oral
- Intravenous
- Intraperitoneal
- Subcutaneous
- Intranasal
- Intratracheal

Models available:
- Brain (Glioma)
- Breast
- Colon
- Head and Neck
- Kidney
- Liver
- Leukemia
- Lung (Carcinoma)
- Lung (Anaplastic Carcinoma)
- Lymphoma (Burkitt’s)
- Melanoma
- Myeloma
- Ovarian
- Pancreas
- Prostate
- Thyroid (Carcinoma)

Cell lines available:
- U87 MG, C6 (rat)
- MDA-MB-231, MCF-7, BT474, MX-1, HN104, VT203
- HT-29, COLO-205, HCC 2998
- FaDu, A253
- 786-0
- MHCC97H, Huh-7
- HL-60, K562, Dal-3b-M2
- H-460, H-358, A549
- Calu-6
- Raji
- A-375, B16-F10 (mouse/syngeneic)
- RPMI-8226
- SK-OV-3, OVCAR-5
- AsPC-1, PANC-1, HPAF-II, BxPC-3, Miapaca-2, MCF-SNCG, U266
- PC-3
- TT

Drug Discovery & Development Services