Vivacell Biotechnology GmbH is a privately owned contract-based research organisation (CRO) that provides specialised in vitro and in vivo models for the development and proving the health benefits of cosmetics, oral and personal care products, nutraceuticals, food and bevarages, pharmaceuticals, probiotcials, natural products, and botanicals.
Additional services for marketing studies are offered.
VivaCell’s preclinical services include standard protocols and protocols adapted to the needs of the customers using a variety of in vitro and in vivo systems.
VivaCell is combining highly qualified cellular and molecular biology knowledge and expertise in various fields.
At VivaCell we offer you complete research projects from basic R&D up to pre-clinical research (GLP) in one hand. Our clients do not have to gather the research data form various CROs.
in vitro platform to test the effects of products on menopause symptoms
Estrogen deficiency in postmenopausal woman usually first manifests as vasomotor symptoms. The hot flushes can be disruptive and disturb sleep patterns, outcomes resulting in fatigue, depression, insomnia, and irritability. Absence of estrogen action occurs at other target sites such as bone, heart, and brain, although clinical manifestations may not become apparent until years later. Because life expectancy is age 80 years for women in Western countries and the average age of menopause is close to age 50 years, approximately 30 years can be associated with an “estrogen-deficiency” state. Use of estrogen in the postmenopausal woman clearly has health benefits but, as with many pharmacological interventions, also has attendant risks. Unopposed estrogen therapy in standard doses is associated with an increased risk of endometrial hyperplasia and cancer.
Thus, there is a huge demand for estrogen replacement therapeutics acting on beta estradiol receptors but not activating alpha estrogen receptors and thus increasing the risk to develop cancer.
We therefore designed a platform to study the effects of products on menopause symptoms including various models and parameters shown to be involved in the symptoms accompanied with menopause.
This platform includes the following parameters:
Modul Activity 1: Effects on receptors/parameters involved in mood problems associated with menopause
1. Serotonin 5-HT1C receptor
2. Serotonin 5-HT2A receptor
3. Serotonin 5-HT2C receptor
4. Serotonin re-uptake
5. Serotonin transporter
Modul Activity 2: Effects on receptors/parameters involved in menopause symptoms hyperthermia and hot flushes
6. Serotonin 5-HT7 receptor
7. Dopamine Receptor (D2)
8. Adrenergic receptor alpha 2
9. Opioid Receptor (Delta, mu and kappa opioid receptors)
11. Agonistic or antagonistic effects of the test item on estradiol receptor alpha (hER)
12. Agonistic or antagonistic effects of the test item on estradiol receptor beta (hER)
13. Effects on breast cancer cell proliferation
14. Agonistic effects of the test items on farnesoid receptor activity (FXR)
Please aks for the study protocol for more details.
Inhibitory effects of ingredients/test items on particulate matter (PM) induced environmental stress (inflammation, oxidative stress) in skin and immune cells
Exposure to atmospheric particulate matter (PM), a component of air pollution, has been associated with significant adverse health effects leading to increased overall morbidity and mortality of worldwide significance. The effects are more pronounced in patients with pre-existing conditions such as asthma, diabetes or obstructive pulmonary disorders. The possibility that PM may affect organs systems sensitive to oxidative stress and inflammatory responses must be considered. The immune system appears to be the most sensitive target organ for environmental pollutants. Inhaled PMs can deposit in different compartments in the respiratory tract and interact with epithelial cells and resident immune cells. Exposed to PMs can induce local or systematic inflammatory responses. Furthermore, among the affected organs besides the immune system, the skin is the primary tissue exposed to ambient pollutants and, similar to the respiratory tract, presents an interface between the body and surrounding atmosphere
We are offering various models to prove the benefits of products (herbal extracts, fractions, compounds etc.) on specific end-points of hair growth and the prevention of hair loss.
• Effects on the proliferation of dermal papilla cells
• Effects on various growth factors in dermal papilla cells (TGF, KGF, IGF, PDGF etc.)
• Effects on caspase-3, 7 and 9 in dermal papilla cells
• Effects on cellular energy levels (ATP) in dermal papilla cells/keratinocytes/fibroblasts
• Effects on VEGF expression in keratinocytes
• Effects on TERT
• Effects on 5-alpha reductase I and II
• Effects on Wnt/-catenin pathway
• Effects on inflammation (TNFalpha, IL-1beta, IL-6)
• Effects on human dermal papilla cells/keratinocytes co-cultures/3D culture (all parameters)
• Other parameters on request
At VivaCell, we have adapted specific protocols “in vitro” to study the anti-tumoural activity of compounds, extracts etrc.. More than 100 parameters including cellular, molecular and biochemical techniques are offered to our client, and by using such parameters we run projects adapted to each drug and tailored to customer’s needs.
In vitro testing methods include proliferation inhibition assays using human tumour cell lines, as well as clonogenic assays with human tumor xenografts, human tumor cell lines, and hematopoetic stem cells. More than 350 solid tumor models have been established in serial passage as well as 20 permanent tumor cell lines (co-operation). In addition, a panel of 60 cell lines, commonly used in cancer research such as A549, MCF-7, Caco-2, HT29 or PC3, are also available. Cancer cell lines overexpressing the MDR1 gene are also available for drug research.
In vivo assays include subcutaneous models, orthotopic models with human xenografts, murine tumour models (co-operation) and models of topic co-carcinogenesis inducing papilloma-like skin tumours in athymic mice.
Angiogenesis(e.g. VEGF, angiopoietin I and II, nephrin, i.e.). In vitro3D assays (spheroids), HUVECs and Matrigel Basement Membrane Matrix, In vivocorneal neovascularization assays, 3D assays (spheroids injected s.c.),
Other specific cellular and molecular techniques are available upon request.
Vivacell Biotechnology GmbH provides specialised in vitro models and assays for testing and developing ingredients, formulations, and end products for the cosmetic, dermato-cosmetic, hair, personal and oral care market.
Our major task is to support clients to prove the health benefits of their products in the following topics.
and many others.
Biochemistry & Molecular Biology Services
Product Development, Testing, and Packaging Services
Lead Identification and Validation Services
Angiogenesis & Endothelial Cell Assays Services
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