Virovek, Inc.

Custom AAV Production

All inclusive package customized to your specific serotype, gene and yield needs, ready in as little as 6 weeks, includes:

• Full cloning service: strategy design, cloning, sequence verification
• Production service: serotype packaging, processing, purification, and titration
   

Regular pricing (per construct), based on production scale:

1x10^13 vg: $3,500
2x10^13 vg: $4,950
5x10^13 vg: $13,950
1x10^14 vg: $19,950
2x10^14 vg: $29,950
5x10^14 vg: $59,950
1x10^15 vg: $119,900
 

Inquire for details on:

• academic and non-profit discounts
• other production scales
   

Optional add-ons:

• Plasmids: $0.32 each
• Leftover storage (up to 12 months): $50
• GLP documentation: $20,000
• GLP MaxiBacmid DNA banks: $10,000
• Lysate: 50% of AAV production cost
• Cell pellet: 50% of AAV production cost
• Empty capsids: $750
• Gene cloning: included

Pre-made AAVs

Ready for Immediate shipping. Default titer 2x10^13 vg, each 100ul starting at $295

Inquire for details on:

• Titer adjustments (prices vary)
• Different promoter and reporter genes
• Latest inventory

This DNA product will allow Baculovirus to generate your gene of interest. We will clone your plasmid into our pFB shuttle plasmid, followed by recombinant bacmid DNA generation, before purify for delivery. 

Optional add-ons:

• Pre-made bacmids: $695
• Glycerol (preparation of 10 vials of glycerol stocks): $200
• Large scale purification (yield 0.5-1.0mg): $2,500

Successfully cloned plasmid into our pFastBac backbone at a volume of 30ug, concentration 4ng/ul, generated by Maxi Prep. Starting at $350

Optional add-ons:

• Gene cloning: $395
• pFB: included

We use baculovirus to infect Sf9 insect cells as an intermediate step that allows us to produce AAV

Scale of 1E+9 pfu: $1,495
Scale of 1E+10 pfu: $2,950 
 

Optional add-ons:

Plaque purification: $3,000

We create constructs of AAV packaging to include toxin genes, IRES or P2A sequences, miRNAs, shRNAs, Cre-dependent, and dual promoter expression cassettes.

Single-step cloning strategies:

• $395
• Excision of the gene of interest and insertion into the destination plasmid
• 1-2 weeks

Complex cloning strategies:

• $250 each additional step
• Ligation of 3+ pieces of DNA, insertion of fusion proteins or linkers
• Up to 4 steps

Quality Control Assays

Choose from the following list, running qPCT Titration and SDS-PAGE for your AAV samples

• Endotoxin: $275
• Host Cell Genomic DNA Contamination: $125
• MOI Optimization: $2,500
• Bac Plaque Purification: $1,500
• qPCR Titration (for separate preps): $125
• SDS-PAGE (for separate preps): $50
• Qubit Protein Measurement: $200
• Aggregation (with report): $350
• Full & Empty (protein & DNA, with report): $400