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Virovek, Inc.

20 Orders Completed
Hayward, California, US

About Virovek, Inc.

Founded: 2006 Type: Privately Held Size: 1-10 employees

Striving for Excellence in Gene Delivery

 

Virovek specializes in large-scale and custom-made adeno-associated virus (AAV) vector production through our innovative and patented BAC-to-AAV and toxin-based cell ablation technologies. Since 2006, thousands of AAV vectors at 1E+13vg scale or above have been produced to support projects in hundreds of university laboratories, non-profit institutions and industry leaders across the Americas, Europe and Asia.

 

Our AAV production platform is able to generate 1E+17vg/ml of virus from a single production run. We offer comprehensive services including:

  • Custom AAV production (1E+13 to 1E+17vg/ml)
  • Pre-made AAVs
  • Plasmids, Bacmids, Recombinant Baculovirus
  • Gene cloning service
  • Quality Control Assays

Serotypes (inquire to confirm the latest availabilities):

  • AAV1
  • AAV2
  • AAV2-Retro
  • AAV3A, 3B
  • AAV4
  • AAV5
  • AAV6
  • AAV7
  • AAV8
  • AAV9
  • AAVShH10
  • AAVrh10
  • AAV7m8
  • AAV-DJ
  • AAVphp.b
  • AAVphp.eb
  • AAV10
  • AAV11

Our mission is to provide the highest quality AAV vectors to support basic science research and clinical stage drug development.

 

Virovek is BSL-2 certified 

 

www.virovek.com

 

Certifications & Qualifications

BSL-2

Our Services (6)


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AAV Production

Adeno-associated virus (AAV) production
Price on request

Custom AAV Production

All inclusive package customized to your specific serotype, gene and yield needs, ready in as little as 6 weeks, includes:

  • Full cloning service: strategy design, cloning, sequence verification
  • Production service: serotype packaging, processing, purification, and titration
     

Regular pricing (per construct), based on production scale:

1x10^13 vg: $3,500
2x10^13 vg: $4,950
5x10^13 vg: $13,950
1x10^14 vg: $19,950
2x10^14 vg: $29,950
5x10^14 vg: $59,950
1x10^15 vg: $119,900
 

Inquire for details on:

  • academic and non-profit discounts
  • other production scales
     

Optional add-ons:

  • Plasmids: $0.32 each
  • Leftover storage (up to 12 months): $50
  • GLP documentation: $20,000
  • GLP MaxiBacmid DNA banks: $10,000
  • Lysate: 50% of AAV production cost
  • Cell pellet: 50% of AAV production cost
  • Empty capsids: $750
  • Gene cloning: included

 

Pre-made AAVs

 

Ready for Immediate shipping. Default titer 2x10^13 vg, each 100ul starting at $295


Inquire for details on:

  • Titer adjustments (prices vary)
  • Different promoter and reporter genes
  • Latest inventory


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Bacmid Preparation

Starting at $695.00 per unit

This DNA product will allow Baculovirus to generate your gene of interest. We will clone your plasmid into our pFB shuttle plasmid, followed by recombinant bacmid DNA generation, before purify for delivery. 


Optional add-ons:

  • Pre-made bacmids: $695
  • Glycerol (preparation of 10 vials of glycerol stocks): $200
  • Large scale purification (yield 0.5-1.0mg): $2,500


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Plasmids and Vectors

Price on request

Successfully cloned plasmid into our pFastBac backbone at a volume of 30ug, concentration 4ng/ul, generated by Maxi Prep. Starting at $350


Optional add-ons:

  • Gene cloning: $395
  • pFB: included


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Baculovirus Production

Price on request

We use baculovirus to infect Sf9 insect cells as an intermediate step that allows us to produce AAV

Scale of 1E+9 pfu: $1,495
Scale of 1E+10 pfu: $2,950 
 

Optional add-ons:

Plaque purification: $3,000


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Custom Gene Cloning

Starting at $395.00 per unit

We create constructs of AAV packaging to include toxin genes, IRES or P2A sequences, miRNAs, shRNAs, Cre-dependent, and dual promoter expression cassettes.


Single-step cloning strategies:

  • $395
  • Excision of the gene of interest and insertion into the destination plasmid
  • 1-2 weeks

 

Complex cloning strategies:

  • $250 each additional step
  • Ligation of 3+ pieces of DNA, insertion of fusion proteins or linkers
  • Up to 4 steps

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Quality Assurance

Price on request

Quality Control Assays

 

Choose from the following list, running qPCT Titration and SDS-PAGE for your AAV samples

  • Endotoxin: $275
  • Host Cell Genomic DNA Contamination: $125
  • MOI Optimization: $2,500
  • Bac Plaque Purification: $1,500
  • qPCR Titration (for separate preps): $125
  • SDS-PAGE (for separate preps): $50
  • Qubit Protein Measurement: $200
  • Aggregation (with report): $350
  • Full & Empty (protein & DNA, with report): $400


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Sharon Chen

Operations Associate
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