ViraQuest Inc. is a biotechnology company specializing in custom adenovirus production services. Our custom services include the production of novel vectors or the amplification of existing stocks. Quality control is included on every lot number. We offer an array of adenovirus services to meet your needs including new constructs, dual-expressing vectors, amplifications and purification. We are committed to providing you with the best research materials and support in the adenovirus field. Let our vectors keep your research headed in the right direction.
AMPLIFICATION- Whether starting out with a new shuttle construct or using a virus stock, viruses are amplified prior to purificaition on CsCl gradients. Amplificaitons typically take less than 2 weeks from reciept of your materials to delivery.
We include 2 mL of CsCl purified adenovirus particles at 1e12 pts/mL. PFU and RCA assays are included.
Aliquoting and custom buffer formulation is available.
RAPAd® technology enables ViraQuest Inc. staff to produce your custom adenovirus particles in as little as 3 weeks.
SUBCLONING – Our team can do the subcloning with the cDNA supplied by your lab or we can procure or synthesize the cDNA for you. Our subcloning services include sequencing the 5’ and 3’ ends or the entire insert if PCR was used as part of the cloning strategy. If you prefer to carry out the subcloning, we can send our shuttle plasmids and provide technical assistance with the cloning strategy. The shuttles have an extensive MCS to facilitate directional subcloning of your insert.
Please see the resources page for plasmid maps.
Reporters include GFP, Luc, b-gal, among others. Virus is supplied at CsCl purified in 1 mL aliquots at 1e12 pts/mL. PFU and RCA assays are available for every lot number.
Virus is CsCl purified and supplied in 1 mL aliquots at 1e12 pts/mL. These are suitable for direct injections in animal experiments or tissue/cell culture.
We typically keep stock viruses on-hand for next day shipment. These include control virus particles (Empty) and Cre, AlkPhos, B-gal, Luciferase and others.
Bulk pricing and aliquoting is available.
We maintain a stock of all virus produced at our facility. We have 100kw generator as an automated power back up supply to keep our freezers cold and incubators warm during any power outage.
Our repository allowed us to replenish materials lost during hurricain Katrina and to individual labs when their freezers went down.
PURIFICATION – All of our virus particles are purified over 2 rounds of CsCl gradients, dialyzed to remove the cesium and stored in one mL aliquots at 1X10^12 particles/mL. Our standard storage buffer contains TRIS buffer with sucrose as the cryoprotectant. These particles are suitable for direct injection for in vivo experiments or cell culture. If you have specific buffer requirements, we can formulate your virus prep in the storage buffer of your choice.
Viraquest has not received any reviews.
Viraquest has not received any endorsements.