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VIRAPUR

San Diego, California, US

VIRAPUR specializes in custom virus production, purification and viral assay services. Our recognized virologists prepare high titer, purified virus for diagnostics, research and development purposes. We have worked with common and obscure viruses and phage classified BSL-2 and -3 at a range of scales. Our quality system assures delivery of reliable virus product. We perform custom in vitro antiviral testing against viruses from our inventory or strains you provide. Follow the links above to learn more.

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Viral Infectivity Assays
Price on request

AAV infectivity assays with helper Adenovirus.
Replication Competent AAV assays

AAV infectivity assays with helper Adenovirus.
Replication Competent AAV assays

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AAV
AAV Production
Adeno-associated virus (AAV) production
Price on request

High titer AAV production at mid-scale

High titer AAV production at mid-scale

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Virus production
Price on request

We grow, titer and purify many viruses from a variety of species. We handle BSL-2 and BSL-3 pathogens in our certified biocontainment labs. We are skilled in making both wild type and recombinant viruses and preparing viral nucleic acids. Below are some of the viruses we have manufactured. If you do not see your virus of interest... Show more »

We grow, titer and purify many viruses from a variety of species. We handle BSL-2 and BSL-3 pathogens in our certified biocontainment labs. We are skilled in making both wild type and recombinant viruses and preparing viral nucleic acids. Below are some of the viruses we have manufactured. If you do not see your virus of interest here, please inquire.

Tissue culture and Egg virus production scales to suit your development and regulatory needs are available. Projects are priced based on scientific and regulatory requirements. No job is too small; we will grow virus from the 10 ml scale to the 16 liter scale.

Human and Animal Adenoviruses and recombinants
Bacteriophage
Baculovirus recombinants
Coronaviruses
Cytomegalovirus (CMV)
Dengue
Enteroviruses
Epstein Barr Virus (EBV)
Herpesviruses 1 and 2, and glycoprotein antigens
Human Poliovirus
Influenza Viruses A and B (current and historical strains) in tissue culture and eggs
Marine Mammal Viruses
Measles
Minute Virus of Mice (MVM)
Mouse Hepatitis
Mouse Polio
Mumps
Parainfluenza
Picornaviruses
Respiratory Syncytial Virus (RSV)
Rhinoviruses
Rubella
SV-40
Vaccinia Virus and recombinants
Varicella Zoster Virus (VZV)
Virus Like Particles (VLP)
Xenotropic Murine leukemia virus-Related Virus (XMRV)

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Antiviral Testing
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VIRAPUR has developed in vitro assays for screening antiviral compounds against panels of human and veterinary viruses. We also customize assays according to your specific requirements. Primary screens include:

  • Inhibition of infection
  • Hemagglutination Inhibition (HAI)
  • Titer reduction assay
  • Plaque inhibition assay
    -... Show more »

VIRAPUR has developed in vitro assays for screening antiviral compounds against panels of human and veterinary viruses. We also customize assays according to your specific requirements. Primary screens include:

  • Inhibition of infection
  • Hemagglutination Inhibition (HAI)
  • Titer reduction assay
  • Plaque inhibition assay
  • Inhibition of specific viral enzymes such as influenza neuraminidase
  • Neutralization of infectivity by antibody or antibody like molecules
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Plaque Assay for Viral Titer
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Determines infectious virus particles in a sample. Virus is sequentially diluted and dilutions are plated on susceptible cells. Cultures are overlaid with nutrient agarose and plaques or pocks formed by cytopathic viruses can be quantitated.

Determines infectious virus particles in a sample. Virus is sequentially diluted and dilutions are plated on susceptible cells. Cultures are overlaid with nutrient agarose and plaques or pocks formed by cytopathic viruses can be quantitated.

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Radial Immunodiffusion Assay
Single Radial Immunodiffusion
Price on request

The SRID Assay is used to quantitate the amount of antigen in a sample. Antigen concentration is determined by the degree of antigen/antibody precipitate around a well into which detergent solubilized virus is placed. A standardized antigen preparation and a specific polyclonal antibody are required.

The SRID Assay is used to quantitate the amount of antigen in a sample. Antigen concentration is determined by the degree of antigen/antibody precipitate around a well into which detergent solubilized virus is placed. A standardized antigen preparation and a specific polyclonal antibody are required.

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Bacteriophage Vector Construction
Price on request

We offer custom construction of recombinant bacteriophage containing your gene of interest.

The similarity between animal viruses and bacteriophage in their structure, physicochemical properties and infection dynamics is striking. Bacteriophage are viruses infecting bacteria and are known to be specific for E. coli,... Show more »

We offer custom construction of recombinant bacteriophage containing your gene of interest.

The similarity between animal viruses and bacteriophage in their structure, physicochemical properties and infection dynamics is striking. Bacteriophage are viruses infecting bacteria and are known to be specific for E. coli, staphylococcus and all bacterial genus. We apply many techniques we have developed in understanding animal viruses to bacteriophage with great results.

  • VIRAPUR has engineered bacteriophage containing control viral genes as lysis controls for viral PCR assays.
  • We can engineer a phage expressing or containing your gene of interest.
  • We can purify phage to pharmaceutical grade to high concentrations and low endotoxin.
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Compound IC50 Assay
Price on request

The IC50 (50% Inhibitory Concentration) of an antiviral drug can be determined using tissue culture. A sample of known virus concentration is incubated with susceptible cells in the presence of increasing concentrations of an antiviral drug. This assay determines the concentration at which the drug will inhibit virus replication.... Show more »

The IC50 (50% Inhibitory Concentration) of an antiviral drug can be determined using tissue culture. A sample of known virus concentration is incubated with susceptible cells in the presence of increasing concentrations of an antiviral drug. This assay determines the concentration at which the drug will inhibit virus replication. This can also be done using antibodies in the place of drugs, in which case it is called a Microneutralization Assay.

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Endpoint Dilution (TCID50) Assay
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Quantitates infectious virus particles in a sample. Virus is sequentially diluted and dilutions are plated in replicate wells of susceptible cells. The TCID50 (50% Tissue Culture Infectious Dose) is that dilution of virus which will produce pathological change in fifty percent of cell cultures inoculated at a particular dilution.... Show more »

Quantitates infectious virus particles in a sample. Virus is sequentially diluted and dilutions are plated in replicate wells of susceptible cells. The TCID50 (50% Tissue Culture Infectious Dose) is that dilution of virus which will produce pathological change in fifty percent of cell cultures inoculated at a particular dilution. TCID50 values are normally very close to the Plaque Assay values for most cytopathic viruses.

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Virus Purification
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Viral Assays
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Adenovirus production
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Microneutralization Assay
Price on request

An excellent test to determine if an antibody may have therapeutic effects. This assay measures the ability of an antiserum to inhibit cytopathogenic effect of about 100 virus infectious units. The antibody/virus mixture is plated on susceptible cells and inhibition is tracked.

An excellent test to determine if an antibody may have therapeutic effects. This assay measures the ability of an antiserum to inhibit cytopathogenic effect of about 100 virus infectious units. The antibody/virus mixture is plated on susceptible cells and inhibition is tracked.

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Hemagglutination Assay
Price on request

Detects the presence of certain viruses that agglutinate red blood cells. This assay can be used to quantitate or type many virus strains. For example, influenza virus possesses the capacity to agglutinate red blood cells of specific animal species. It does this by cross linking sialic acid residues on the surface of red blood... Show more »

Detects the presence of certain viruses that agglutinate red blood cells. This assay can be used to quantitate or type many virus strains. For example, influenza virus possesses the capacity to agglutinate red blood cells of specific animal species. It does this by cross linking sialic acid residues on the surface of red blood cells. In some labs, the hemagglutination assay is preferred for detecting influenza virus growth. The hemagglutination titer of an influenza virus sample can be determined when dilutions of virus are mixed with human or turkey red blood cells. The presence of virus will hold the red cells in a diffuse matrix and prevent them from settling out to the bottom of the well.

Hemagglutination Inhibition (HI/HAI) Assay
This test is based on the inhibition of viral agglutination by a specific antibody and can be used for virus identification or for assay of antibodies. This assay is still used as a standard for measuring the efficacy of influenza vaccines and potency of neutralizing antibodies.

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Biology
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Microbiology, Virology, and Parasitology
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Viral Production and Assays
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Viral Packaging
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Biochemistry & Molecular Biology
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Protein Services
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Antibody Services
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Nucleic Acid Services
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DNA
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Plasmids and Vectors
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Pharmacology & Toxicology
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Toxicology
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Cytotoxicity Assays
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2018-04-24 23:54:54 -0700

Net Promoter Score of 10 received for Antiviral Testing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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