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Viral Vector Core at La Jolla

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San Diego, California, US

About Viral Vector Core at La Jolla

The Viral Vector core facility at Sanford Burnham Prebys Medical Discovery Institute develops state-of-the-art viral vector-based gene delivery technology. Its portfolio ranges from lentivirus, retrovirus, adenovirus, sindbis virus, AAV, and VSV products as well as customized “The Works” viral vector... Show more »

The Viral Vector core facility at Sanford Burnham Prebys Medical Discovery Institute develops state-of-the-art viral vector-based gene delivery technology. Its portfolio ranges from lentivirus, retrovirus, adenovirus, sindbis virus, AAV, and VSV products as well as customized “The Works” viral vector construction and swapping service package. There are a variety of ready-to-transduce viral vector collections including constitutively expressed or inducible fluorescent proteins for nuclear or membrane localization and bioluminescence reporters for 3D therapeutic target and drug screening. The viral core now integrates CRISPRs/Cas technology in a plasmid-free viral platform including lentivirus, adenovirus and AAV, providing scientists with choices of combined technologies to manipulate genes using CRISPR gene disruption, editing, inhibition, and activation. More importantly, the core actively participates and supports translational research to fight human diseases.

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Our Services (17)


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Lentivirus Production

Price on request
  1. Custom packaged lenti-viral (HIV-1 and FIV) production is based on constructs provide by the customer. Titration is offered for virus ubiquitously expressing fluorescent marker.

  2. Ready-to-transduce lentiviral preps - include: (1) constitutively expressed or inducible fluorescent protein, antibiotic selection marker, or... Show more »

  1. Custom packaged lenti-viral (HIV-1 and FIV) production is based on constructs provide by the customer. Titration is offered for virus ubiquitously expressing fluorescent marker.

  2. Ready-to-transduce lentiviral preps - include: (1) constitutively expressed or inducible fluorescent protein, antibiotic selection marker, or bioluminescence reporter (2) nuclear or membrane localization tool (3) constitutively expressed non-silencing shRNA control.
    • hPGK-eGFP
    • hPGK-mCherry
    • hPGK-H2B-eGFP (nuclear eGFP)
    • hPGK-H2B-mCherry (nuclear mCherry)
    • hPGK-tdTomato
    • hPGK-Firefly Luciferase
    • hEF1α- Firefly Luciferase
    • UBC-eGFP-Firefly Luciferase fusion
    • CMV-Renilla-Luciferase
    • Tet-inducible-eGFP
    • Tet-inducible-tdTomato
    • CMV-NLS-eGFP (nuclear eGFP)
    • CMV-NLS-mCherry (nuclear mCherry)
    • CMV-eGFP-CAAX (membrane eGFP)
    • CMV-mCherry-CAAX (membrane mCherry)
    • LKO.1-scrambled shRNA (Sigma-Aldrich)
    • GIPZ-scrambled shRNA (Open Biosystems)
    • TRIPZ-scrambled shRNA (Open Biosystems)

  3. "The Works" custom lentiviral vector construction – offers vector generation based on cDNA or shRNA of interests with a choice of constitutive/inducible expression vectors, viral production, and titration.

  4. Custom viral particles concentration and purification with user-provided viral supernatants allows customers to increase viral titer (up to 1000 fold) based on crude viral supernatants.

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Retroviral Production

Price on request
  1. Custom packaged retro-viral (MMLV) production is based on constructs provide by the customer. Titration is offered for virus ubiquitously expressing fluorescent marker.

  2. Ready-to-transduce retroviral preps - include: constitutively expressed fluorescent protein.
    • GFP
    • mCherry

  1. Custom packaged retro-viral (MMLV) production is based on constructs provide by the customer. Titration is offered for virus ubiquitously expressing fluorescent marker.

  2. Ready-to-transduce retroviral preps - include: constitutively expressed fluorescent protein.
    • GFP
    • mCherry

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Adenovirus Production

Price on request
  1. Custom production of adenoviral particles (Ad5) from user-supplied construct or virus - includes: (1) adenovirus recovery (2) high purity adenoviral preparation using CsCl gradient purification (3) titration using the plaque assay.

  2. "The Works" custom lentiviral-adenoviral vector construction and swapping - includes: (1)... Show more »

  1. Custom production of adenoviral particles (Ad5) from user-supplied construct or virus - includes: (1) adenovirus recovery (2) high purity adenoviral preparation using CsCl gradient purification (3) titration using the plaque assay.

  2. "The Works" custom lentiviral-adenoviral vector construction and swapping - includes: (1) constitutive expression of custom cDNAs (2) inducible shRNA of interests expressed under a new Tet inducible system (3) Custom promoters/enhancer reporter vectors (4) MicroRNA sponges.

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AAV Production

Adeno-associated virus (AAV) production
Price on request
  1. "The Works" custom AAV vector construction and virus packaging
    • Serotype Tropism
     AAV-1 : SM, CNS, retina, pancreas
     AAV-2 : In vitro, VSMC, SM, CNS, liver, kidney
     AAV-3 : Hepatocarcinoma, SM
     AAV-4 : CNS, retina, lung
     AAV-5 : SM, CNS, lung, retina
     AAV-6 : SM, SM (i.v.),... Show more »
  1. "The Works" custom AAV vector construction and virus packaging
    • Serotype Tropism
     AAV-1 : SM, CNS, retina, pancreas
     AAV-2 : In vitro, VSMC, SM, CNS, liver, kidney
     AAV-3 : Hepatocarcinoma, SM
     AAV-4 : CNS, retina, lung
     AAV-5 : SM, CNS, lung, retina
     AAV-6 : SM, SM (i.v.), heart, lung
     AAV-8 : Liver, SM, CNS, retina, pancreas, heart
     AAV-DJ : In vitro, liver, heart, kidney
     AAV-DJ/8 : liver, heart, kidney, pancreas, SM, brain (i.v.)

• Ready-to-transduce AAV
 AAV-2-GFP
 AAV-DJ-GFP

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CRISPR/Cas9 Vector Construction

Price on request
  1. All-in-one CRISPR/Cas gene disruption and editing viral vectors
    • Knock-out using viral vector platform (hCas9 + gRNA)
     Gene disruption using adenovirus or lentivirus (on demand)
    Example: T7EI assay, gRNA-T1 to 3 targeting Rosa26 loci
    Example: Ready-to-transduce Ad5-CRISPR all-in-1 targeting GFP
    ... Show more »
  1. All-in-one CRISPR/Cas gene disruption and editing viral vectors
    • Knock-out using viral vector platform (hCas9 + gRNA)
     Gene disruption using adenovirus or lentivirus (on demand)
    Example: T7EI assay, gRNA-T1 to 3 targeting Rosa26 loci
    Example: Ready-to-transduce Ad5-CRISPR all-in-1 targeting GFP

    • Knock-in using plasmid-free viral vector platform (hCas9 + gRNA + Donor DNA)
     Gene editing using adenovirus (on demand)
     Human AAVS1 safe harbor loci
     Mouse Rosa26 safe harbor loci
    Example: knock-in GFP-2A-Gene of interests in Rosa26 loci

  2. Genetic modified mouse creation
    • Knock-in (hCas9 + gRNA + Donor DNA)
     Rosa26 loci
     Choice of C57BL/6 C2 and 129 mESC.
    Example: knock-in GFP-2A-Gene of interests in Rosa26 loci
    gRNA#1: 4 out of 4 screened mESC clonal line(100%) show Rosa loci integration
    gRNA#2: 3 out of 4 screened mESC clonal line (75%) show Rosa loci integration
    gRNA#3: 2 out of 4 screened mESC clonal line (50%) show Rosa loci integration

  3. Custom CRISPR gene inhibition (CRISPRi) and activation (CRISPRa)
    • CRISPRi viral vector delivery
     Adenovirus (dCas9-Krab)
     Lentivirus (dCas9-Krab and gRNA)
    Example: SOX2 CRISPRi

    • CRISPRa viral vector delivery
     Lentivirus (dCas9-VP64 and gRNA)

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In vitro Reporter Gene Assays

Price on request
  1. Custom lenti- and adeno-viral reporter for 2D and 3D screening
    • One secreted luciferase is constitutively expressed by hPGK promoter for normalization.
    • Another secreted luciferase is expressed by your promoter of interest.
    • The third secreted marker is expressed by another promoter of interest, your choice.
    Example: GFAP expression*
  1. Custom lenti- and adeno-viral reporter for 2D and 3D screening
    • One secreted luciferase is constitutively expressed by hPGK promoter for normalization.
    • Another secreted luciferase is expressed by your promoter of interest.
    • The third secreted marker is expressed by another promoter of interest, your choice.
    Example: GFAP expression*
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Large Scale Plasmid Prep

Price on request
  1. Large scale DNA plasmid preparation (ready for viral production) starts from a small amount of customer's vector (e.g. miniprep) or transformed bacterial stock.
  1. Large scale DNA plasmid preparation (ready for viral production) starts from a small amount of customer's vector (e.g. miniprep) or transformed bacterial stock.
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VSV Production

Vesicular Stomatitis Virus Production
Price on request
  1. "The Works" custom VSV vector construction and production
     Pseudotyped VSV-ΔG-GFP
     Ebola virus glycoprotein (EboV-GP) Zaire strain (Kissidougou-C15, 2014)
     Vesicular stomatitis virus glycoprotein (VSV-G)
    Example: VSV/Lenti-GFP-EboV-GP expression
  1. "The Works" custom VSV vector construction and production
     Pseudotyped VSV-ΔG-GFP
     Ebola virus glycoprotein (EboV-GP) Zaire strain (Kissidougou-C15, 2014)
     Vesicular stomatitis virus glycoprotein (VSV-G)
    Example: VSV/Lenti-GFP-EboV-GP expression
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Virus Production

Price on request
  1. Custom packaged Sindbis viral vector production is based on constructs provide by the customer.
  1. Custom packaged Sindbis viral vector production is based on constructs provide by the customer.
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Plasmids and Vectors

Price on request
Request a quote for more information about this service.

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Biochemistry & Molecular Biology

Price on request
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Microbiology

Price on request
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Nucleic Acid Services

Price on request
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Virology Services

Price on request
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Cell-Based Assays

Price on request
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DNA Services

Price on request
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Biology

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Chun-Teng Huang

Associate Director
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Science_Exchange_Standard_MTA_SBMRI 23_ Jan 2015.pdf

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