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Cornell University Veterinary Molecular Diagnostics

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Ithaca, New York, US

About Cornell University Veterinary Molecular Diagnostics

The Animal Health Diagnostic Center (AHDC) at Cornell University offers a wide array of testing capabilities to detect markers of health and disease in animals. It is one of the most comprehensive veterinary diagnostic laboratories in the country, whose faculty provide subject matter expertise and... Show more »

The Animal Health Diagnostic Center (AHDC) at Cornell University offers a wide array of testing capabilities to detect markers of health and disease in animals. It is one of the most comprehensive veterinary diagnostic laboratories in the country, whose faculty provide subject matter expertise and oversight of more than 900 individual assays, performed by 200 highly trained technical staff. The AHDC services include large and small-scale testing for academic and industry research and development. Our sophisticated and comprehensive diagnostic capabilities include anatomic pathology (necropsy and histopathology services), clinical pathology (hematology, hemostasis, cytology, endocrinology), nutritional and toxicology testing. Infectious disease sections span all relevant disciplines including bacteriology, mycology, parasitology, and virology with a wide range of culture capabilities and available cell lines. The molecular diagnostics laboratory has high-throughput capacity for identifying defined and novel pathogenic and non-pathogenic organisms, mutation detection for genetic disease, and whole genome sequencing of bacterial isolates. The AHDC excels in assay customization and integrated diagnostic support, with flexibility to meet the needs of clients engaged in basic and applied research and development.

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Our Services (3)


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Veterinary Molecular Diagnostics

Price on request

Our laboratory is equipped with state-of-the-art equipment and facilities including high-throughput automated extraction and liquid handling systems and a variety of platforms for PCR and next-generation sequencing. We operate within a Quality Assurance System that complies with the American Association of Veterinary Laboratory... Show more »

Our laboratory is equipped with state-of-the-art equipment and facilities including high-throughput automated extraction and liquid handling systems and a variety of platforms for PCR and next-generation sequencing. We operate within a Quality Assurance System that complies with the American Association of Veterinary Laboratory Diagnosticians. The lab also undergoes proficiency testing conducted by the National Veterinary Services Laboratory and the US Food and Drug Administration.

Services Provided:

• Pathogen load in animal specimens by qPCR using the ABI 7500 and OpenArray platforms
• Whole genome sequencing of bacterial isolates on the Illumina MiSeq platform
• Environmental surveillance for Salmonella using enrichment rtPCR
• Tick testing for up to 14 different pathogens by qPCR
• Digital PCR on the Biorad QX200 ddPCR system

Typical sample requirements:

• Feces weighed into 400mg aliquots in snap-cap microcentrifuge tubes and frozen at -80
• Respiratory swabs in sterile, sealed vials with several drops of saline added
• Ticks frozen or preserved in ethanol
• Contact us for other animal or environmental specimen types

Deliverables:

• qPCR/rtPCR results will be provided in Excel spreadsheets with Ct values (and copy number
interpolations if requested)
• Bacterial WGS - fastq files and/or draft genomes provided by FTP or uploaded to NCBI

Recent publications highlighting our work:

• Wagner et al. Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age. PLoS One 2017 http://dx.plos.org/10.1371/journal.pone.0169072
• Goodman LB et al. High-throughput detection of respiratory pathogens in animal specimens by nanoscale PCR. Journal of Visualized Experiments. 2016 Nov 28;(117) http://bit.ly/2gRLsn0

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Applied Biosystems 7500 Fast Real-Time PCR system Bio-Rad QX200 droplet digital PCR KingFisher FLEX QuantStudio 12K Flex Applied Biosystems OpenArray Goat Dog Horse Cat Birds Cow Lyme disease Influenza Cryptosporidiosis Leptospirosis Salmonellosis Johne's disease Bovine respiratory disease complex Canine respiratory disease complex Feline respiratory disease complex qPCR WGS ddPCR real-time PCR Blood Frozen Tissue Stool Biofluids Show 28 more tags Show less

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Veterinary Research & Diagnostics

Price on request

Veterinary Research & Diagnostic Services

Veterinary Research & Diagnostic Services

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Veterinary Laboratory Services

Price on request

Veterinary Laboratory Services

Veterinary Laboratory Services

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