Qloxpj2r6uvwx0odur4q vector

VectorBuilder, Inc.

Santa Clara, California, US

VectorBuilder is a revolutionary O2O platform for vector design and custom cloning. Our online design tool provides a highly intuitive workflow for you to design your desired vector with just a few mouse clicks, all for free. You can then use our online ordering system to purchase the custom cloning of your vector – along with related services such as virus packaging – as easily as shopping on Amazon. The cost and turnaround time of these services are just a fraction of DIY.

Shortly after its launch in 2015, VectorBuilder has quickly become the world's leading provider of vector cloning and virus packaging services. VectorBuilder has won many awards for its highly innovative platform. To date, VectorBuilder have served tens of thousands of researchers in thousands of universities and companies worldwide, and has been cited in numerous scientific papers.

The creators of VectorBuilder believe that a vector is... Show more »

VectorBuilder is a revolutionary O2O platform for vector design and custom cloning. Our online design tool provides a highly intuitive workflow for you to design your desired vector with just a few mouse clicks, all for free. You can then use our online ordering system to purchase the custom cloning of your vector – along with related services such as virus packaging – as easily as shopping on Amazon. The cost and turnaround time of these services are just a fraction of DIY.

Shortly after its launch in 2015, VectorBuilder has quickly become the world's leading provider of vector cloning and virus packaging services. VectorBuilder has won many awards for its highly innovative platform. To date, VectorBuilder have served tens of thousands of researchers in thousands of universities and companies worldwide, and has been cited in numerous scientific papers.

The creators of VectorBuilder believe that a vector is just a reagent, not a research project. Yet, we see too often that biologists, especially budding trainees, spend countless hours toiling in the lab trying to clone a few vectors, sometimes unsuccessfully after months of hard work. We believe this to be a colossal waste of human potential. We therefore decided to create VectorBuilder, a platform that makes vector construction easy, fast, affordable – and yes, fun – for researchers around the world.

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VectorBuilder, Inc. has not listed any services.

shRNA Plasmid Construction
Price on request

Overview

Mammalian shRNA Knockdown Vectors
U6-Based
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- PiggyBac

miR30-Based
- Regular plasmid
- Lentivirus
- Adenovirus
- Adeno-associated Virus (AAV)
- PiggyBac
- Tol2

Others
- Lentivirus shRNA sensor (for testing shRNA specificity)
- Lentivirus shRNA knockdown and sensor

Overview

Mammalian shRNA Knockdown Vectors
U6-Based
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- PiggyBac

miR30-Based
- Regular plasmid
- Lentivirus
- Adenovirus
- Adeno-associated Virus (AAV)
- PiggyBac
- Tol2

Others
- Lentivirus shRNA sensor (for testing shRNA specificity)
- Lentivirus shRNA knockdown and sensor

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Plasmid DNA Preparation
Price on request

Services Available

Endo-free maxiprep (DNA)
- >1 ug/ul, 300 ul, 1x TE buffer, endotoxin-free, sterile
- ~6 day turnaround

Miniprep (DNA)
- >200 ng/ul, 50 ul, 1x TE buffer)
- ~5 day turnaround

Services Available

Endo-free maxiprep (DNA)
- >1 ug/ul, 300 ul, 1x TE buffer, endotoxin-free, sterile
- ~6 day turnaround

Miniprep (DNA)
- >200 ng/ul, 50 ul, 1x TE buffer)
- ~5 day turnaround

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Plant Genetic Engineering
Price on request

Custom Plant Vectors Available
- Agrobacterium binary vector
- Regular plasmid

Custom Plant Vectors Available
- Agrobacterium binary vector
- Regular plasmid

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CRISPR/Cas9 Vector Construction
Price on request

CRISPR Vectors Available

gRNA and Cas9 Coexpression
Single gRNA
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- Regular plasmid

Dual gRNA
- Lentivirus
- Adenovirus
- Regular plasmid

gRNA Only
Single gRNA
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- PiggyBac
-... Show more »

CRISPR Vectors Available

gRNA and Cas9 Coexpression
Single gRNA
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- Regular plasmid

Dual gRNA
- Lentivirus
- Adenovirus
- Regular plasmid

gRNA Only
Single gRNA
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- PiggyBac
- Regular plasmid

Dual gRNA
- Lentivirus
- Adenovirus
- PiggyBac
- Regular plasmid

Cas9 Only
- Lentivirus
- Adenovirus
- Adeno-associated virus (AAV)
- PiggyBac
- Regular plasmid

CRISPR-Based Gene Activation
- Lentivirus Synergistic Activation Mediator (SAM)

CRISPR-Based Gene Inhibition
- Regular plasmid gRNA sensor (for testing gRNA specificity)

HR Donor Vectors (for CRISPR Targeting)
- KO Donor Vectors
- KI Donor Vectors

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AAV Production
Adeno-associated virus (AAV) production
Price on request

Adeno-associated virus (AAV) Packaging
- Simply provide us with a vector map and/or sequence and our staff will QC your AAV vector and perform virus packaging at one of three available scales suitable for either in-vitro or in-vivo applications:

Packaging Scales
- Medium Scale: >10e11 GC/ml, 1 ml
- Large Scale:... Show more »

Adeno-associated virus (AAV) Packaging
- Simply provide us with a vector map and/or sequence and our staff will QC your AAV vector and perform virus packaging at one of three available scales suitable for either in-vitro or in-vivo applications:

Packaging Scales
- Medium Scale: >10e11 GC/ml, 1 ml
- Large Scale: >10e12 GC/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e12 GC/ml, 1 ml

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Adenovirus Production
Price on request

Adenovirus Packaging
- Simply provide us with a vector map and/or sequence and our staff will QC your adenoviral vector and perform virus packaging at one of three available scales suitable for either in-vitro or in-vivo applications:

Packaging Scales
- Medium Scale: >10e10 PFU/ml, 1 ml
- Large Scale: >10e11... Show more »

Adenovirus Packaging
- Simply provide us with a vector map and/or sequence and our staff will QC your adenoviral vector and perform virus packaging at one of three available scales suitable for either in-vitro or in-vivo applications:

Packaging Scales
- Medium Scale: >10e10 PFU/ml, 1 ml
- Large Scale: >10e11 PFU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e12 VP/ml, 2 ml

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Lentivirus Production
Price on request

Lentivirus Packaging
- Simply provide us with a vector map and/or sequence and our staff will QC your lentiviral vector and perform virus packaging at one of three available scales suitable for either in-vitro or in-vivo applications:

Packaging Scales
- Medium Scale: >10e8 TU/ml, 1 ml
- Large Scale: >10e9 TU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e9 TU/ml, 1 ml

Lentivirus Packaging
- Simply provide us with a vector map and/or sequence and our staff will QC your lentiviral vector and perform virus packaging at one of three available scales suitable for either in-vitro or in-vivo applications:

Packaging Scales
- Medium Scale: >10e8 TU/ml, 1 ml
- Large Scale: >10e9 TU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e9 TU/ml, 1 ml

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Custom Lentiviral Vectors
Price on request

As the world's largest provider of custom vector cloning services. VectorBuilder can make virtually any vector tailored to your research needs. Our vector systems have been optimized through extensive R&D work, and all have been validated by in vitro and, where applicable, in vivo testing. Besides cloning, we also offer... Show more »

As the world's largest provider of custom vector cloning services. VectorBuilder can make virtually any vector tailored to your research needs. Our vector systems have been optimized through extensive R&D work, and all have been validated by in vitro and, where applicable, in vivo testing. Besides cloning, we also offer many related services such as virus packaging, BAC modification, and library construction.

Vector Construction Services Are Available By:
- You can design your own vectors using our highly intuitive free online vector design tool VectorBuilder.com. This tool is very easy to learn and use, and you can design a vector in just a minute or two. To use the tool, click the Design My Vector button on the homepage.
- If you are unable to use our online tool to design your desired vector or if you have other service needs, just send us a request. Our experienced scientists will design your vector for free and address your other service needs.
- The deliverables of our cloning services are E. coli stocks containing the vectors you ordered. You also can add downstream services such as plasmid DNA preparation and virus packaging.

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Virus Production
Price on request

VectorBuilder has developed a range of proprietary technologies and reagents that greatly improved virus packaging protocols in terms of titer, purity, viability and consistency. Our packaging protocols are also optimized for the viral vector systems used in our cloning services. As a result, we have a growing base of highly... Show more »

VectorBuilder has developed a range of proprietary technologies and reagents that greatly improved virus packaging protocols in terms of titer, purity, viability and consistency. Our packaging protocols are also optimized for the viral vector systems used in our cloning services. As a result, we have a growing base of highly satisfied customers who come back to us time after time for their cloning and virus packaging needs.

Virus Packaging Services Available
Lentivirus
- Medium Scale: >10e8 TU/ml, 1 ml
- Large Scale: >10e9 TU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e9 TU/ml, 1 ml

_ Adenovirus_
- Medium Scale: >10e10 PFU/ml, 1 ml
- Large Scale: >10e11 PFU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e12 VP/ml, 2 ml

AAV
- Medium Scale: >10e11 GC/ml, 1 ml
- Large Scale: >10e12 GC/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e12 GC/ml, 1 ml

MMLV
- Medium Scale: >10e7 TU/ml, 1 ml
- Large Scale: >10e8 TU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e8 TU/ml, 1 ml

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Plasmid Construction
Price on request
Request a quote for more information about this service.
Vector Construction and Optimization
Price on request

VectorBuilder.com is a revolutionary online platform for vector cloning. Researchers can use VectorBuilder’s highly intuitive online tool to design their desired vectors, and order custom cloning services for these vectors along with related services such as virus packaging. Cost and turnaround time are a fraction of DIY
**100%... Show more »

VectorBuilder.com is a revolutionary online platform for vector cloning. Researchers can use VectorBuilder’s highly intuitive online tool to design their desired vectors, and order custom cloning services for these vectors along with related services such as virus packaging. Cost and turnaround time are a fraction of DIY
100% Sequence Guaranteed
- Your vector is guaranteed to match the requested sequence 100% or we will re-synthesize it for free. Silent/synonymous mutations may be present to assist with cloning, but must be approved by client.

Endo-free maxipreps and virus packaging
- Easily add endo-free maxipreps and virus packaging (in-vitro and in-vivo grade) to any vector construction order

Available Vector Systems
- Mammalian Gene Expression Vectors
- Mammalian Non-coding RNA Expression Vectors
- Mammalian shRNA Knockdown Vectors
- Mammalian CRISPR Vectors
- HR Donor Vectors (for CRISPR Targeting)
- Enhancer/Promoter Testing Vectors
- Zebrafish Gene Expression Vectors
- Drosophila Gene Expression Vectors
- Plant Gene Expression Vectors
- Recombinant Protein Expression Vectors
- In Vitro Transcription Vectors
- And more!

Modify existing vectors
- Simply provide us with a vector map and/or sequence for your vector, and we will be happy to modify your existing vector to insert or remove any number of features upon request

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Cloning Vectors
Price on request

As the world's largest provider of custom vector cloning services. VectorBuilder can make virtually any vector tailored to your research needs. Our vector systems have been optimized through extensive R&D work, and all have been validated by in vitro and, where applicable, in vivo testing. Besides cloning, we also offer... Show more »

As the world's largest provider of custom vector cloning services. VectorBuilder can make virtually any vector tailored to your research needs. Our vector systems have been optimized through extensive R&D work, and all have been validated by in vitro and, where applicable, in vivo testing. Besides cloning, we also offer many related services such as virus packaging, BAC modification, and library construction.

« Show less
Viral Packaging
Price on request

VectorBuilder has developed a range of proprietary technologies and reagents that greatly improved virus packaging protocols in terms of titer, purity, viability and consistency. Our packaging protocols are also optimized for the viral vector systems used in our cloning services. As a result, we have a growing base of highly... Show more »

VectorBuilder has developed a range of proprietary technologies and reagents that greatly improved virus packaging protocols in terms of titer, purity, viability and consistency. Our packaging protocols are also optimized for the viral vector systems used in our cloning services. As a result, we have a growing base of highly satisfied customers who come back to us time after time for their cloning and virus packaging needs.

Virus Packaging Services Available
Lentivirus
- Medium Scale: >10e8 TU/ml, 1 ml
- Large Scale: >10e9 TU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e9 TU/ml, 1 ml

Adenovirus
- Medium Scale: >10e10 PFU/ml, 1 ml
- Large Scale: >10e11 PFU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e12 VP/ml, 2 ml

AAV
- Medium Scale: >10e11 GC/ml, 1 ml
- Large Scale: >10e12 GC/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e12 GC/ml, 1 ml

MMLV
- Medium Scale: >10e7 TU/ml, 1 ml
- Large Scale: >10e8 TU/ml, 1 ml
- Ultra-purified Scale (in-vivo): >10e8 TU/ml, 1 ml

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BAC Generation
Price on request

VectorBuilder can fulfill all your BAC modification needs with rapid turnaround and unbeatable prices. We can place reporters behind regulatory sequences on the BAC, introduce point mutations into genes of interest, transfer regions of the BAC onto a plasmid, and add drug-selection or visualization markers to the BAC backbone.

VectorBuilder can fulfill all your BAC modification needs with rapid turnaround and unbeatable prices. We can place reporters behind regulatory sequences on the BAC, introduce point mutations into genes of interest, transfer regions of the BAC onto a plasmid, and add drug-selection or visualization markers to the BAC backbone.

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Plasmids and Vectors
Price on request

VectorBuilder.com is a revolutionary online platform for vector cloning. Researchers can use VectorBuilder’s highly intuitive online tool to design their desired vectors, and order custom cloning services for these vectors along with related services such as virus packaging. Cost and turnaround time are a fraction of DIY
**100%... Show more »

VectorBuilder.com is a revolutionary online platform for vector cloning. Researchers can use VectorBuilder’s highly intuitive online tool to design their desired vectors, and order custom cloning services for these vectors along with related services such as virus packaging. Cost and turnaround time are a fraction of DIY
100% Sequence Guaranteed
- Your vector is guaranteed to match the requested sequence 100% or we will re-synthesize it for free. Silent/synonymous mutations may be present to assist with cloning, but must be approved by client.

Endo-free maxipreps and virus packaging
- Easily add endo-free maxipreps and virus packaging (in-vitro and in-vivo grade) to any vector construction order

Available Vector Systems
- Mammalian Gene Expression Vectors
- Mammalian Non-coding RNA Expression Vectors
- Mammalian shRNA Knockdown Vectors
- Mammalian CRISPR Vectors
- HR Donor Vectors (for CRISPR Targeting)
- Enhancer/Promoter Testing Vectors
- Zebrafish Gene Expression Vectors
- Drosophila Gene Expression Vectors
- Plant Gene Expression Vectors
- Recombinant Protein Expression Vectors
- In Vitro Transcription Vectors
- And more!

Modify existing vectors
- Simply provide us with a vector map and/or sequence for your vector, and we will be happy to modify your existing vector to insert or remove any number of features upon request

« Show less
shRNA Library Construction
Price on request

Summary
VectorBuilder offers high-quality pooled shRNA libraries targeting human and mouse genes. For each species, we provide ready-to-use lentivirus libraries at two scales: Whole Genome (~19,000 RefSeq genes) and Elite Gene (~2,000 most frequently cited genes on PubMed Central). Where possible, each gene is targeted by 5-6... Show more »

Summary
VectorBuilder offers high-quality pooled shRNA libraries targeting human and mouse genes. For each species, we provide ready-to-use lentivirus libraries at two scales: Whole Genome (~19,000 RefSeq genes) and Elite Gene (~2,000 most frequently cited genes on PubMed Central). Where possible, each gene is targeted by 5-6 different shRNAs. These libraries can serve as powerful and cost-efficient tools for performing genome-wide loss-of-function screens in mammalian cells.

Genome-wide targeting and high complexity
- For both human and mouse, we offer shRNA libraries at two scales: Whole Genome (~19,000 RefSeq genes) and Elite Gene (~2,000 most frequently cited genes on PubMed Central). On average, every gene is targeted by 5-6 different shRNAs with good knockdown scores calculated based on a set of rules following the guideline of the RNAi consortium (TRC). This enables more reliable knockdown and more effective screening.

High uniformity
- The pooled plasmid libraries were validated by next-generation sequencing (NGS), which successfully recovered 100% of shRNAs in the Elite Gene libraries and >97% of shRNAs in the Whole Genome libraries. In addition, shRNA representation is highly uniform in the plasmid libraries.

Well-established lentiviral vector and high titer lentivirus that is ready to use
- The pooled shRNA libraries are expressed in the third-generation lentiviral vector system driven by human U6 promoter, which is a highly efficient system for stably knocking down expression of target genes in a wide variety of cells. This lentiviral vector system is ideal for in vitro genetic screens since it can introduce shRNAs into cells permanently, efficiently and uniformly. All pooled shRNA libraries are provided as ready-to-use lentivirus with high functional titer (>10e8 TU/ml), which saves your time in virus packaging and titer measurement. The third-generation lentiviral vector system is optimized for improved biosafety given its incompetent self-replication feature.

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RNA Services
Price on request

RNA Services

RNA Services

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Agricultural Discovery & Development
Price on request

Agricultural Discovery & Development Services

Agricultural Discovery & Development Services

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Agriscience
Price on request

Agriscience Services

Agriscience Services

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Virology Services
Price on request

Viral Production and Assays Services

Viral Production and Assays Services

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Microbiology
Price on request

Microbiology, Virology, and Parasitology Services

Microbiology, Virology, and Parasitology Services

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Molecular Cloning
Price on request

Molecular Cloning Services

Molecular Cloning Services

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DNA Libraries
Price on request

DNA Libraries Services

DNA Libraries Services

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DNA Services
Price on request

DNA Services

DNA Services

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Nucleic Acid Services
Price on request

Nucleic Acid Services

Nucleic Acid Services

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Biochemistry & Molecular Biology
Price on request

Biochemistry & Molecular Biology Services

Biochemistry & Molecular Biology Services

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Biology
Price on request

Biology Services

Biology Services

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siRNA Synthesis
Price on request
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Open Reading Frame Cloning
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Codon Optimization
Price on request
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cDNA Cloning
Price on request
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cDNA Library Construction
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Retroviral Production
Price on request
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Cells and Tissues
Price on request
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Genetic Engineering
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BAC Libraries
Price on request
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shRNA Synthesis
Price on request
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Lentiviral sgRNA Library Construction
Price on request
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Reporter Gene mRNA
Price on request
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Bacterial Artificial Chromosome (BAC)
Price on request
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Vector-Based miRNA
Price on request
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Vector-Based siRNA
Price on request
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DNA Amplification
Price on request
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Customized Non-Mutant Library
Price on request
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Non-Coding RNA Synthesis
Price on request
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Synthetic DNA Libraries
Price on request
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Design of Genetic Constructs
Price on request
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ORF Clones
Price on request
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Stock Viruses
Price on request
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gRNA Synthesis
Price on request
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Viral Vector Purification
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Drug Discovery & Development
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Neuroscience
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DNA Library Preparation
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Structural Biology
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Custom siRNA Library
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rAAV Production
Recombinant Adeno-Associated Virus Products
Price on request
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2018-07-06 13:26:36 -0700

Net Promoter Score of 7 received for Plasmid Construction.

Additional Ratings: satisfaction with deliverable: 7, satisfaction with timeliness: 6.

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